A5047949241- HIV Research and Treatment
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Cytomegalovirus and herpesvirus research
- HIV/AIDS drug development and treatment
- Viral-associated cancers and disorders
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Herpesvirus Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Angiogenesis and VEGF in Cancer
- Respiratory viral infections research
- Immunodeficiency and Autoimmune Disorders
- HIV-related health complications and treatments
- SARS-CoV-2 detection and testing
- HIV/AIDS Research and Interventions
- Lymphatic System and Diseases
- Immune Response and Inflammation
- Virus-based gene therapy research
- COVID-19 epidemiological studies
- Lymphoma Diagnosis and Treatment
- Antibiotic Resistance in Bacteria
- Animal Virus Infections Studies
University of Brescia
2016-2025
Universitatea de Medicină, Farmacie, Științe și Tehnologie „George Emil Palade” din Târgu Mureș
2024-2025
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
2015-2024
University of Maryland, Baltimore
2002-2023
Dompé (Italy)
2023
Brescia University
1996-2022
Università degli Studi della Tuscia
2021
Hudson Institute
2020
John Wiley & Sons (United States)
2017-2020
Centro di Riferimento Oncologico
2018
Coronavirus disease 2019 (COVID-19) infection has the potential for targeting central nervous system, and several neurological symptoms have been described in patients with severe respiratory distress. Here, we case of a 60-year-old patient acute syndrome coronavirus 2 (SARS-CoV-2) but only mild abnormalities who developed an akinetic mutism attributable to encephalitis. Magnetic resonance imaging was negative, whereas electroencephalography showed generalized theta slowing. Cerebrospinal...
(1) Background: Acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiological agent for disease (COVID-19) that has led to a pandemic began in March 2020. The role of SARS-CoV-2 components on innate and adaptive immunity still unknown. We investigated possible implication pathogen-associated molecular patterns (PAMPs)-pattern recognition receptors (PRRs) interaction. (2) Methods: infected Calu-3/MRC-5 multicellular spheroids (MTCSs) with clinical strain evaluated activation RNA...
The expression of activation antigens, namely CD25, CD69, CD71, and HLA-DR on T cells from 15 healthy individuals stimulated with different mitogens specific antigens was evaluated by immunofluorescence assay flow cytometric analysis compared cell proliferation as a function [3H]thymidine incorporation. CD69 the earliest expressed antigen cells, while latest. Regardless stimulus used, lymphocytes expressing CD25 CD71 were always more numerous than HLA-DR. Variations in proportion CD4+ CD8+...
Monoclonal antibodies (MAbs) of predefined specificity have been generated by utilizing a synthetic peptide reflecting amino acid positions 10-17 the Hu-rasT24 gene product as immunogen. These MAbs, designated RAP-1 through RAP-5 (RA, ras; P, peptide), shown to react with ras p21. Since Hu-ras reactive determinants (positions 10-17) predicted be within tertiary structure p21 molecule, it was not unexpected that denaturation cell extracts or tissue sections Formalin glutaraldehyde enhanced...
Abstract Introduction Carcinoma-associated fibroblasts (CAFs) play a pivotal role in cancer progression by contributing to invasion, metastasis and angiogenesis. Solid tumors possess unique microenvironment characterized local hypoxia, which induces gene expression changes biological features leading poor outcomes. Hypoxia Inducible Factor 1 (HIF-1) is the main transcription factor that mediates cell response hypoxia through different mechanisms include regulation of genes strongly...
Several preclinical and clinical investigations have argued for nervous system involvement in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Some sparse case reports described various forms of encephalitis disease 2019 (COVID-19) disease, but very few data focused on presentations, course, response to treatment, outcomes.The SARS-CoV-2 related encephalopaties (ENCOVID) multicenter study included patients with full infectious screening, cerebrospinal fluid (CSF),...
Antibody detection plays a pivotal role in the diagnosis of pathogens and monitoring success vaccine immunization. However, current serology techniques require multiple, time-consuming washing incubation steps, which limit their applicability point-of-care (POC) diagnostics high-throughput assays. We developed here nucleic acid nanoswitch platform able to instantaneously measure immunoglobulins type G E (IgG IgE) levels directly blood serum other bodily fluids. The system couples advantages...
Lineage B.1.617+, also known as G/452R.V3 and now denoted by WHO with the Greek letters δ κ, is a recently described SARS-CoV-2 variant under investigation first identified in October 2020 India. As of May 2021, three sublineages labeled B.1.617.1 (κ), B.1.617.2 (δ), B.1.617.3 have been already identified, their potential impact on current pandemic being studied. This has 13 amino acid changes, its spike protein, which are currently particular concern: E484Q, L452R, P681R. Here, we report...
Vascular diseases supported by aberrant angiogenesis have increased incidence in HIV-1-infected patients. Several data suggest that endothelium dysfunction relies on action of HIV-1 proteins rather than a direct effect the virus itself. The matrix protein p17 is known to deregulate biological activity different immune cells. Recently, was found mimic IL-8 chemokine binding receptor CXCR1. Here we show binds with high affinity CXCR2, CXCR1-related receptor, and promotes formation...
Summary Current leukaemia therapy focuses on increasing chemotherapy efficacy. Mesenchymal stromal cells ( MSC s) have been proposed for carrying and delivery drugs to improve killing of cancer cells. We shown that s loaded with Paclitaxel PTX ) acquire a potent anti‐tumour activity. investigated the effect human hMSC mouse SR 4987 hMSCsPTX MOLT ‐4 L 1210, two cell LCs lines origin, respectively. showed strong anti‐LC , co‐injected or intra‐tumour injected into established subcutaneous...
Significance Non-Hodgkin lymphomas (NHLs) are associated with HIV-1 infection, but the HIV1 genome is not detectable in malignant B cells. Here we show that variants of matrix protein p17 (vp17s) detected NHL specimens HIV+ patients. These vp17s more frequently patients than without NHL. display a potent B-cell growth-promoting activity, which exerted by activating Akt signaling pathway. Results obtained CD spectroscopy and thermal denaturation suggest mutation-induced destabilization may...
The aim of this study is the characterization and genomic tracing by phylogenetic analyses 59 new SARS-CoV-2 Italian isolates obtained from patients attending clinical centres in North Central Italy until end April 2020. All but one newly-characterized genomes belonged to lineage B.1, most frequently identified European countries, including Italy. Only a single sequence was found belong B. A mean 6 nucleotide substitutions per viral genome observed, without significant differences between...
Since the first outbreak of SARS-CoV-2, clinical characteristics Coronavirus Disease 2019 (COVID-19) have been progressively changed. Data reporting a viral intra-host and inter-host evolution favouring appearance mild SARS-CoV-2 strains are since being accumulating. To better understand pathogenicity its adaptation to host, it is therefore crucial investigate genetic phenotypic circulating lately in epidemic.Nasopharyngeal swabs analyzed for load early (March 2020) late (May phases epidemic...
Abstract Covid-19 infection has the potential for targeting central nervous system and several neurological symptoms have been described in patients with severe respiratory distress. Here we case of a 60-year old subject SARS-CoV-2 but only mild abnormalities who developed an akinetic mutism due to encephalitis. MRI was negative whereas EEG showed generalized theta slowing. CSF analyses during acute stage were SARS-CoV-2, positive pleocytosis hyperproteinorrachia, increased IL-8 TNF-α...
Integrins represent a gateway of entry for many viruses and the Arg-Gly-Asp (RGD) motif is smallest sequence necessary proteins to bind integrins. All Severe Acute Respiratory Syndrome Virus type 2 (SARS-CoV-2) lineages own an RGD (aa 403-405) in their receptor binding domain (RBD). We recently showed that SARS-CoV-2 gains access into primary human lung microvascular endothelial cells (HL-mECs) lacking Angiotensin-converting enzyme (ACE2) expression through this conserved motif. Following...