A5060354343- Listeria monocytogenes in Food Safety
- Vector-Borne Animal Diseases
- Salmonella and Campylobacter epidemiology
- Viral Infections and Vectors
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Genomics and Phylogenetic Studies
- Vector-borne infectious diseases
- Identification and Quantification in Food
- Brucella: diagnosis, epidemiology, treatment
- SARS-CoV-2 detection and testing
- Antibiotic Resistance in Bacteria
- Microbial infections and disease research
- Insect and Pesticide Research
- Molecular Biology Techniques and Applications
- Spectroscopy and Chemometric Analyses
- Parasite Biology and Host Interactions
- Mosquito-borne diseases and control
- Mycobacterium research and diagnosis
- Microbial Inactivation Methods
- Respiratory viral infections research
- Essential Oils and Antimicrobial Activity
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2016-2025
University of Teramo
2023
Azienda USL di Pescara
2023
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche
2021
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
2021
Istituto Zooprofilattico Sperimentale delle Venezie
2021
University of Bari Aldo Moro
2021
Philadelphia University
2008
University of Padua
2007
University of Messina
1997
Following the announcement on December 2020 about emergence of a new variant (VOC 202012/ 01, B.1.1.7 lineage) in United Kingdom, targeted surveillance was put place Abruzzo region (Italy), which allowed detection 313 persons affected by lineage B.1.1.7, up to 20th February 2021. We investigated results RT-PCR nasopharyngeal swabs tested from 2021 verify any difference viral load and persistence between people infected others. Statistically significant lower values CT associated with N...
Biting midges (Culicoides spp.) are important vectors of several insect borne arboviruses but underrepresented in terms availability high-resolution genomic resources. We assembled and annotated complete mitochondrial genomes for two Culicoides species, namely C. brevitarsis imicola which proven Bluetongue Virus (BTV). used both long read short sequencing technologies to assemble the circular genomes. The genome sizes 17,100 bp 17,031 bp, respectively, all comprising 37 genes, including 13...
In recent years, novel Bluetongue virus (BTV) serotypes have been isolated and/or sequenced by researchers within the field. During surveillance activities, we identified a putative BTV serotype in healthy goats from Sardinia, Italy. RNAs purified blood and serum samples were positive for generic real time RT-PCR c-ELISA, respectively, whereas genotyping serotyping unsuccessful. By NGS, whole genome sequence was obtained two (BTV-X ITL2015 strains 34200 33531). Overall, Seg 2 of BTV-X shows...
The use of whole-genome sequencing (WGS) using next-generation (NGS) technology has become a widely accepted method for microbiology laboratories in the application molecular typing outbreak tracing and genomic epidemiology. Several studies demonstrated usefulness WGS data analysis through single-nucleotide polymorphism (SNP) calling from reference sequence Brucella melitensis, whereas gene-by-gene comparison core-genome multilocus (cgMLST) not been explored so far. current study developed...
The current pandemic is caused by a novel coronavirus (CoV) called SARS-CoV-2 (species Severe acute respiratory syndrome-related coronavirus, subgenus Sarbecovirus, genus Betacoronavirus, family Coronaviridae). In Italy, up to the 2nd of April 2020, overall 139,422 confirmed cases and 17,669 deaths have been notified, while 26,491 people recovered. Besides overloading hospitals, another issue face was capacity perform thousands tests per day. this perspective, support National Health Care...
Italy was one of the first countries to experience a major epidemic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), with >1000 cases confirmed by 1 March 2020. However, virus genome sequence data is sparse and there has been only limited investigation transmission across country. Here, we provide most extensive study date genomic epidemiology SARS-CoV-2 in covering wave infection. We generated 191 new full-length genomes, largely sampled from central (Abruzzo), before, during,...
Abstract We detected severe acute respiratory syndrome coronavirus 2 in an otherwise healthy poodle living with 4 family members who had disease. observed antibodies serum samples taken from the dog, indicating seroconversion. Full-length genome sequencing showed that canine and human viruses were identical, suggesting human-to-animal transmission.
Abstract It is unclear whether West Nile virus (WNV) circulates between Africa and Europe, despite numerous studies supporting an African origin high transmission in Europe. We integrated genomic data with geographic observations phylogenetic phylogeographic inferences to uncover the spatial temporal viral dynamics of WNV these two continents. focused our analysis towards lineages 1 (L1) 2 (L2), most spatially widespread pathogenic lineages. Our study shows a Northern-Western L1,...
Injured tendons are challenging in their regeneration; thus, tissue engineering represents a promising solution. This research tests the hypothesis that response of amniotic epithelial stem cells (AECs) can be modulated by fiber diameter size tendon biomimetic fleeces. Particularly, effect electrospun poly(lactide-co-glycolide) (PLGA) fleeces with highly aligned microfibers possessing two different sizes (1.27 and 2.5 µm: ha1- ha2-PLGA, respectively) was tested on ability AECs to...
In this rapid communication, a novel atypical bluetongue virus (BTV) strain detected in goats the Piedmont region (north-western Italy) is described. This strain, BTV-Z ITA2017, most related Seg-2/VP-2 (83.8% nt/82.7% aa) to TOV of BTV-25. Reactive antisera positive by cELISA for BTV antibodies failed neutralize chimeric expressing outermost protein TOV. Infected animals displayed low levels RNAemia and absence clinical signs consistent with infection, scenario described infected strains.
Italy’s second wave of SARS-CoV-2 has hit hard, with more than three million cases and over 100,000 deaths, representing an almost ten-fold increase in the numbers reported by August 2020. Herein, we present analysis 6515 sequences sampled Italy between 29 January 2020 1 March 2021 show how different lineages emerged multiple times independently despite lockdown restrictions. Virus lineage B.1.177 became dominant variant November 2020, when peaked at 40,000 a day, but since this is being...
African swine fever (ASF) is responsible for important socio-economic effects in the global pig industry, especially countries with large-scale piggery sectors. In January 2022, virus (ASFV) genotype II was identified a wild boar population mainland Italy (Piedmont region). This study describes molecular characterization, by Sanger and next-generation sequencing (NGS), of first index case 632/AL/2022 another isolate (2802/AL/2022) reported same month, close proximity to first, following...
Introduction The Delta variant posed an increased risk to global public health and rapidly replaced the pre-existent variants worldwide. In this study, genetic diversity spatio-temporal dynamics of 662 SARS-CoV2 genomes obtained during wave across Tunisia were investigated. Methods Viral whole genome partial S-segment sequencing was performed using Illumina Sanger platforms, respectively lineage assignemnt assessed Pangolin version 1.2.4 scorpio 3.4.X. Phylogenetic phylogeographic analyses...
The emergence of SARS-CoV-2 variants has led to several cases among children. However, limited information is available from North African countries. This study describes the strains circulating in Tunisian pediatric population during successive waves. A total 447 complete sequences were obtained individuals aged 13 days 18 years, between March 2020 and September 2022: 369 generated this 78 ones, GISAID, previously patients. These compared with 354 274 ones adults a global dataset,...
African swine fever (ASF), characterized by high mortality rates in infected animals, remains a significant global veterinary and economic concern, due to the widespread distribution of ASF virus (ASFV) genotype II across five continents. In this study, ASFV strains collected Italy during 2022-2023 from two geographical clusters, North-West (Alessandria) Calabria, were fully sequenced. addition, an vivo experiment pigs was performed. Complete genomic sequencing 30 revealed large-fragment...
Abstract Background Primary Immunodeficiency disorders (PID) can increase the risk of severe COVID-19 and prolonged infection. This study investigates duration SARS-CoV-2 excretion genetic evolution virus in pediatric PID patients as compared to immunocompetent (IC) patients. Materials methods A total 40 nasopharyngeal 24 stool samples were obtained from five ten IC children. RNA detection was performed using RT-qPCR, whole-genome sequencing conducted with NexSeq 1000 platform. Data analysis...
ABSTRACT Members of the RNA virus order Nidovirales infect hosts ranging from marine invertebrates to terrestrial mammals. As such, understanding determinants host range in this group viruses, as well their patterns emergence and disease potential, is clear importance. The Mesoniviridae are a recently documented family within . To date, mesoniviruses have only been associated with infection arthropod species, particularly mosquitoes, hence regarded insect-specific viruses (ISVs). Herein, we...
The continuous emergence of SARS-CoV-2 variants and subvariants poses significant public health challenges. latest designated subvariant JN.1, with all its descendants, shows more than 30 mutations in the spike gene. JN.1 has raised concerns due to genomic diversity potential enhance transmissibility immune evasion. This study aims analyse molecular characteristics JN.1-related lineages (JN.1*) identified Italy from October 2023 April 2024 evaluate neutralization activity against a subsample...