A5043165591- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Streptococcal Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Respiratory viral infections research
- Antibiotic Resistance in Bacteria
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Helicobacter pylori-related gastroenterology studies
- Pneumocystis jirovecii pneumonia detection and treatment
- SARS-CoV-2 and COVID-19 Research
- Antimicrobial Resistance in Staphylococcus
- Eosinophilic Esophagitis
- Microscopic Colitis
- Gastroesophageal reflux and treatments
- Infectious Diseases and Tuberculosis
- Orthopedic Infections and Treatments
- Esophageal and GI Pathology
- Immunodeficiency and Autoimmune Disorders
- Antibiotics Pharmacokinetics and Efficacy
- Microbial Inactivation Methods
- Neonatal Respiratory Health Research
- Cystic Fibrosis Research Advances
- Nosocomial Infections in ICU
Faculté de médecine de Tunis
2007-2025
Children's Hospital
2012-2025
Tunis El Manar University
2015-2025
Tunis University
2007-2024
Hopital Universitaire Hedi Chaker
2020-2024
Hôpital d'Enfants
2002-2022
Hôpital La Rabta
2021
Boston Children's Hospital
2011-2012
Manouba University
2010
Candida albicans chronically colonizes the respiratory tract of patients with Cystic Fibrosis (CF). It competes CF-associated pathogens (e.g. Pseudomonas aeruginosa) and contributes to disease severity. We hypothesize that C. undergoes specific adaptation mechanisms explain its persistence in CF lung environment. To identify underlying genetic phenotypic determinants, we serially recovered 146 clinical isolates over a period 30 months from sputum 25 antifungal-naive patients. Multilocus...
Introduction The Delta variant posed an increased risk to global public health and rapidly replaced the pre-existent variants worldwide. In this study, genetic diversity spatio-temporal dynamics of 662 SARS-CoV2 genomes obtained during wave across Tunisia were investigated. Methods Viral whole genome partial S-segment sequencing was performed using Illumina Sanger platforms, respectively lineage assignemnt assessed Pangolin version 1.2.4 scorpio 3.4.X. Phylogenetic phylogeographic analyses...
The emergence of SARS-CoV-2 variants has led to several cases among children. However, limited information is available from North African countries. This study describes the strains circulating in Tunisian pediatric population during successive waves. A total 447 complete sequences were obtained individuals aged 13 days 18 years, between March 2020 and September 2022: 369 generated this 78 ones, GISAID, previously patients. These compared with 354 274 ones adults a global dataset,...
Background/Objectives: Streptococcus pneumoniae is a leading respiratory pathogen responsible for significant morbidity and mortality, particularly among vulnerable populations. Understanding its antimicrobial resistance patterns serotype distribution crucial guiding treatment prevention strategies. This study aims to examine these trends in S. isolates from Tunisia over two-decade period (2000–2019). Methods: A retrospective time series analysis was conducted on data (n = 4284) gathered...
Pertussis remains a public health concern in most countries. Our study aimed to prospectively explore the epidemiology of pertussis Tunis area Tunisia between 2007 and 2016, characterize virulence-associated genes collected Bordetella isolates.Infants children hospitalized at Children's Hospital Tunis, Tunisia, 2016 for suspicion were enrolled study. Culture real-time PCR (qPCR) assays targeting IS481, IS1001, recA, H-IS1001 ptxP used confirm diagnosis. Phenotypic genotypic characterization...
The prevalence of plasmid-mediated quinolone resistance genes [qnr, aac(6')-Ib-cr and qepA] was sought among Enterobacteriaceae strains obtained from the Children's Hospital Tunis (Tunisia). Non-duplicate isolates (n = 278) with to extended-spectrum cephalosporins collected in 2003, 2007, 2008 2009 were screened for qnr genes. Forty (14.4 %) positive presence qepA qnrB detected 21 Klebsiella pneumoniae, 11 Escherichia coli 6 Enterobacter cloacae isolates. Sequence analysis amplicons revealed...
Due to low susceptibility of coronavirus disease 2019 (COVID-19) in children, limited studies are available regarding COVID-19 the pediatric population Tunisia. The current study evaluated incidence, clinical characteristics, and outcomes severe acute respiratory syndrome type 2 (SARS-CoV-2) infection among children hospitalized at Béchir Hamza Children's Hospital. A retrospective cohort analysis was conducted using hospital database between March 2020 February 2022 with aged ≤15 years...
The worldwide burden of disease bacterial meningitis remains high, despite the decreasing incidence following introduction routine vaccination campaigns.The aim our study was to evaluate epidemiological and bacteriological profile paediatric (BM) in Tunisian children, during period 2003-2019, implementation Haemophilus influenzae type b (Hib) vaccine (April 2011) before 10-valent pneumoccocal conjugate (PCV10) childhood immunization program.All bacteriologically confirmed cases BM admitted...
Neisseria meningitidis, a leading cause of bacterial meningitis and other serious infections, is responsible for approximately one-third cases in the Children's Hospital Tunis. The serogroup distribution, antibiotic susceptibility antigenic molecular characteristics N. meningitidis isolates were determined patients aged 3 days-13 years between February 1998 June 2013. In all 107 invasive strains isolated. Reduced to penicillin G was seen 55.7% isolates, with low level resistance cases; 28.4%...
Pertussis remains a public health concern in most countries. This cross-sectional study aims to investigate the distribution of pertussis toxin antibodies (anti-PT IgG) Tunisian children and adolescents aged 3-18 years, define optimal age for booster vaccination. Anti-PT IgG concentrations enrolled participants were measured using commercial enzyme-linked immunosorbent assay. Concentrations classified as: indicative current/recent infection if ⩾100 IU/ml, recent exposure Bordetella within...
ObjectivesThe aim of this study was to characterize Neisseria meningitidis (Men) isolates in Tunisian paediatric patients with invasive meningococcal disease (IMD) order target therapeutic and preventive strategies.MethodsFifty-nine Men four cerebrospinal fluid samples that were culture-negative but Men-positive by PCR (NC-MenPPCR) (2009–2016) collected from IMD patients. Isolates analysed for their antimicrobial susceptibility. Whole-genome sequencing (WGS) used multilocus sequence typing...
Aim: The aims of our study were to characterize phenotypically and genotypically erythromycin-resistant Streptococcus pyogenes or group A streptococci (ERGAS) isolates, evaluate macrolide resistance analyze the association between emm types virulence factors. Included in this all ERGAS strains isolated from 2000 2013 at Children's hospital Tunis. Antimicrobial susceptibility was performed according CA-SFM guidelines. Macrolide genes revealed by polymerase chain reaction (PCR) method....
Introduction: Rhinoviruses (HRV) are among the leading causes of Severe Acute Respiratory Infection (SARI). Their burden and genetic diversity vary from one region to another little is known in Northern African regions. This study describes epidemiological patterns genotypic HRV SARI cases during a two half year’s study, Tunisia.
 Methodology: A total 271 cases, admitted into Pediatric Intensive Care Unit Bechir Hamza Children's Hospital Tunis, were collected between September 2015...