A5043695253- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- SARS-CoV-2 detection and testing
- Viral Infections and Vectors
- Cytomegalovirus and herpesvirus research
- COVID-19 and healthcare impacts
- Congenital Diaphragmatic Hernia Studies
- Parvovirus B19 Infection Studies
- Neonatal Respiratory Health Research
- Long-Term Effects of COVID-19
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- Vector-Borne Animal Diseases
- Poxvirus research and outbreaks
- Herpesvirus Infections and Treatments
- Vaccine Coverage and Hesitancy
- Single-cell and spatial transcriptomics
- COVID-19 and Mental Health
- Bacteriophages and microbial interactions
- Lipoproteins and Cardiovascular Health
- Biosensors and Analytical Detection
- Travel-related health issues
Policlinico San Matteo Fondazione
2021-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2020-2025
University of Pavia
2017-2025
In August 2023, six locally acquired dengue virus 1 infections were detected in Lodi province, Lombardy Region, northern Italy, where the vector Aedes albopictus is present. Four cases hospitalised, none died. The viruses clustered with Peruvian and Brazilian strains collected between 2021 2023. This preliminary report highlights importance of continued integrated surveillance imported vector-borne potential for tropical disease outbreaks highly populated regions Italy competent vectors are
Echovirus 11 (E11) has recently been associated with a series of nine neonatal cases severe hepatitis in France. Here, we present caused by E11 pair twins. In one the neonates, clinical picture evolved to fulminant hepatitis. The genome showed 99% nucleotide identity strains reported Rapid characterisation using next generation sequencing is essential identify new and more pathogenetic variants.
In an epidemiologic investigation of Enterovirus (EV) infections in a Verona hospital, September 2022-September 2024, we detected EV-C105 six pediatric patients with upper respiratory symptoms between March and May 2023. The primary objective was to describe the local incidence EV cases. secondary perform Sanger's genomic characterization whole-genome sequencing (WGS) EV-C105. proportion positive results calculated based on routine molecular method testing. An available cohort 114 underwent...
Abstract Echovirus 11 (E11) has gained attention owing to its association with severe neonatal infections. Due the limited data available, World Health Organization (WHO) considers public health risk general population be low. The present study investigated genetic variation and molecular evolution of E11 genomes collected from May December 2023. Whole genome sequencing (WGS) was performed for 16 strains. Phylogenetic analysis on WG showed how all Italian strains belonged genogroup D5,...
Rhinovirus is one of the most common respiratory viruses, causing both upper and lower tract infections. It affects mainly children could cause prolonged infections, especially in immunocompromised patients. Here we report our data on a 15-month surveillance seasonality circulation Lombardy Region, Italy. All rhinovirus/enterovirus-positive samples were amplified with RT-PCR for VP4-VP2 region to assign correct genotype. The median age RV/EV-positive patients 9 years, range 0-96. RV-A RV-C...
To assess influenza viruses (IVs) circulation and to evaluate A(H3N2) molecular evolution during the 2021-2022 season in Italy.
We compared the characteristics and outcomes of vaccinated nonvaccinated patients hospitalized with COVID-19.We analyzed in a COVID hub during three one-month periods: (i) October 15, 2020-November 2020 (prevaccination peak); (ii) 2021-November 2021 (Delta wave); (iii) December 2021-January 2022 (Omicron wave). To define epidemiologic context, SARS-CoV-2 infection healthcare workers was analyzed.SARS-CoV-2 incidence 146 cases per 1000 persons (prevaccination) 67 (postvaccination, when...
Mpox virus (MPXV) has recently spread outside of sub-Saharan Africa. This large multicentre study was conducted in Lombardy, the most densely populated Italian region accounting for more than 40% cases. The present aims to: i) evaluate presence and shedding duration MPXV DNA different body compartments correlating viability with time to onset symptoms; ii) provide evidence persistence compartment as a source infection iii) characterize evolution by whole genome sequencing (WGS) during...
Following the pandemic restrictions, epidemiology of respiratory syncytial virus (RSV) has changed, leading to intense hospitalization peaks. This study, conducted at multiple sites in Italy, aimed describe temporal dynamics two post-COVID-19 RSV epidemics. Additionally, circulating RSV-A and -B lineages were characterized compared those found 2018 2019. Respiratory specimens data collected from RSV-positive patients, both inpatients, outpatients, all ages three north-central Italy. To...
ABSTRACT Background Human enterovirus D68 (EV-D68) has been associated with an increase in mild-to-severe pediatric respiratory diseases worldwide. The rate of circulation this virus is largely underestimated the population and genetic evolutionary data are usually available only for partial sequences (e.g VP1 region). To achieve a timely genomic surveillance, reliable, high-throughput EV-68 sequencing assay required. Here we report improved EV-D68 whole-genome (WGS) performed directly on...
The dengue virus (DENV) is a mosquito-borne flavivirus endemic to many tropical and subtropical regions. Over the past few decades, global incidence of has risen dramatically, with now present in over 100 countries, putting nearly half world’s population at risk. This increase attributed several factors, including urbanization, climate change, travel, which facilitate spread both its mosquito vectors. While primarily associated regions, outbreaks temperate areas are becoming increasingly...
SARS-CoV-2 still represents a global health burden, causing more than six million deaths worldwide. Moreover, the emergence of new variants has posed issues in terms vaccine efficacy and immunogenicity. In this study, we aimed to evaluate neutralizing antibody response against different cohorts vaccinated unvaccinated subjects. Four-fold diluted sera from naïve recovered subjects with two or three doses BNT162b2 were challenged 14 variants, titer was measured. Results compared those obtained...
Abstract The pediatric population seems to be at a lower risk of developing severe clinical symptoms COVID‐19. However, the and epidemiological characteristics COVID‐19 in children are yet fully clarified. This retrospective observational study aimed evaluate frequency laboratory‐confirmed patients from February 2020 April 2021. A total 740 (5.1% total) cases were observed during period. peak was November 2020, with 239 cases. During first wave pandemics, 0.89% (49/5877 cases), ranging 0.6%...
Aim: To evaluate a label-free and variant-independent assay called direct virus capture (DVC) for detection of intact SARS-CoV-2 virions through light scattering, utilizing an optical inverted laser microscope nano eye device detector (NED-VD). Methods: The DVC involves the interaction between ACE2 receptors printed on glass coverslip substrate, S protein outer surface virions. study was conducted using 191 human swab specimens. Results: In comparison to RT-PCR assay, method achieved...
The European Center for Disease Prevention and Control has reported 19 cases of severe echovirus 11 infections in neonates since 2022, nine which were fatal. We report a new fatal neonatal case that occurred male twin we evaluated the respiratory intestinal mucosal innate immune response.
We report a considerable increase in enterovirus D68 (EV-D68) cases since July 2024, culminating an ongoing outbreak of acute respiratory infections northern Italy, accounting for nearly 90% all infections. The was identified by community- and hospital-based surveillance systems, detecting EV-D68 individuals with mild-to-severe These strains belonged to B3 divergent A2 lineage. An adult observed. Enhanced molecular characterisation across Europe are needed.