A5003944517- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- COVID-19 diagnosis using AI
- Bacterial Identification and Susceptibility Testing
- Mycobacterium research and diagnosis
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Tuberculosis Research and Epidemiology
- Antibiotics Pharmacokinetics and Efficacy
- Streptococcal Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Antibiotic Use and Resistance
- COVID-19 Impact on Reproduction
- Virology and Viral Diseases
- Antifungal resistance and susceptibility
- Biosensors and Analytical Detection
- COVID-19 and healthcare impacts
- Fungal Infections and Studies
- Vaccine Coverage and Hesitancy
- Genital Health and Disease
- Vibrio bacteria research studies
- Electrolyte and hormonal disorders
- Cervical Cancer and HPV Research
Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda
2014-2025
Ospedale Maggiore
2022-2025
Azienda Ospedaliero-Universitaria Careggi
2022
University of Milan
2020
Azienda Socio Sanitaria Territoriale Lariana
2020
In the coronavirus (CoV) disease 2019 (COVID-19) pandemic, highly selective serological testing is essential to define exposure severe acute respiratory syndrome CoV 2 (SARS-CoV-2). Many tests have been developed, yet with variable speeds first results, and are of unknown quality, particularly when considering prediction neutralizing capacity. The LIAISON SARS-CoV-2 S1/S2 IgG assay was designed measure antibodies against native proteins in a standardized automated chemiluminescence assay.
Abstract From February to April 2020, Lombardy (Italy) reported the highest numbers of SARS-CoV-2 cases worldwide. By analyzing 346 whole genomes, we demonstrate presence seven viral lineages in Lombardy, frequently sustained by local transmission chains and at least two likely have originated Italy. Six single nucleotide polymorphisms (five them non-synonymous) characterized sequences, none affecting N-glycosylation sites. The lineages, clusters within three them, revealed that community...
Since SARS-CoV-2-based disease (COVID-19) spreads as a pandemic, the necessity of highly sensitive molecular diagnosis that can drastically reduce false negatives reverse transcription PCR (rtPCR) results, raises major clinical need. Here we evaluated performance ddPCR-based assay to quantify SARS-CoV-2 titer in 55 suspected COVID-19 cases with negative rtPCR results thanks in-house ddPCR (targeting RdRp and host RNaseP). Samples were collected at ASST-GOM Niguarda between February May 2020...
Carbapenem-Resistant Gram-Negative Bacteria, including Enterobacterales (CRE) and Pseudomonas aeruginosa (CRPA), are common causes of infections in intensive care units (ICUs) Italy. This prospective observational study evaluated the epidemiology, management, microbiological characterization, outcomes hospital-acquired CRE or CRPA treated selected ICUs The included patients with due to 20 from June 2021 February 2023. primary endpoint was 1-year incidence CRE/CRPA infections. Secondary...
Recently, systems have been developed to create total laboratory automation for clinical microbiology. These allow the of specimen processing, incubation, and imaging bacterial growth. In this study, we used WASPLab validate software that discriminates segregates positive negative chromogenic methicillin-resistant Staphylococcus aureus (MRSA) plates by recognition pigmented colonies. A 57,690 swabs submitted MRSA screening were enrolled in study. Four sites specimens following their standard...
Reverse Transcription-Polymerase Chain Reaction (RT-PCR) for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-COV-2) diagnosis currently requires quite a long time span. A quicker and more efficient diagnostic tool in emergency departments could improve management during this global crisis. Our main goal was assessing the accuracy of artificial intelligence predicting results RT-PCR SARS-COV-2, using basic information at hand all departments.This is retrospective study carried out...
Abstract Background Recent studies showed that plasma SARS-CoV-2 RNA seems to be associated with worse COVID-19 outcome. However, whether specific population can at higher risk of viremia are date unexplored. Methods This cross-sectional proof-of-concept study included 41 SARS-CoV-2-positive adult individuals (six affected by haematological malignancies) hospitalized two major hospital in Milan, for those demographic, clinical and laboratory data were available. load was quantified ddPCR...
Vaccination against SARS-CoV-2 has been demonstrated to be safe during gestation. Nevertheless, there are no robust data investigating the entity of maternal antibodies’ transmission through placenta newborn and persistence antibodies in babies’ serum. The objective this study is assess antibody kinetics among newborns first months life. Women having received one or two doses anti-SARS-CoV-2 mRNA-vaccines pregnancy at any gestational age, their newborns, were recruited followed-up over 9...
The polymorphism of the HLA system has been extensively studied in COVID-19 infection, however there are no data about role on vaccine response. We report here HLA-A, -B, -C, and DRB1 allelic frequencies n = 111 individuals after BNT162b2 mRNA vaccine, selected basis lower antibody levels (<5% percentile) second dose among a total 2569 vaccinees, compare them with reference population. found that differences alleles HLA-A*03:01, A*33:03, B*58:01 at least one haplotype...
Tuberculosis (TB), caused by the Mycobacterium tuberculosis complex (MTBC), remains a major global health challenge. Whole genome sequencing (WGS) offers an invaluable tool for understanding genetic diversity and drug resistance profiles of MTBC. This study provides comprehensive WGS dataset 2,520 MTBC isolates collected from four Italian regions-Lombardy, Piedmont, Emilia-Romagna, Lazio-between 2017 2020. The includes genomic data along with associated metadata, such as geographic location...
Background Mycoplasma genitalium (MG) is responsible for non-gonococcal urethritis. Our aim to describe MG positivity rate and incidence in specific populations. Methods Retrospective, surveillance study included all samples collected from 2018 2022. All were tested with Anyplex II STI7 (Seegene). Incidence (IR) was calculated, ratios (IRRs) assessed using Poisson regression model. Results The 13,504 belonging 7,692 individuals. Overall 2.9% (95%CI 2.6–3.2). Subgroups showed significant...
Background: Immunosuppressed patients still exhibit a high mortality rate due to SARS-CoV-2 infection, up 21%. Persistent viral load replication and protracted symptoms result in risk of developing pneumonia, potential antiviral resistance, subsequent delay onco-hematological treatments. Methods: Hematological kidney transplant with treated at GOM Niguarda Hospital (Milan) combined therapy (remdesivir plus nirmatrelvir/ritonavir standard doses) between November 2022 March 2024, were...
Vancomycin-resistant enterococci (VRE) are a major cause of healthcare-associated infections (HAIs). However, the clinical significance VRE colonization and subsequent risk infection in hospitalized patients not fully established. Prolonged hospital stays have been observed neonates colonized by VRE. The mortality rate pediatric with ranges from 0% to 42% both endemic outbreak settings, often occurring VRE-colonized neonates. Host bacterial factors associated worse outcome understood yet. We...
To evaluate the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) anti-spike (S) IgG antibody production after vaccination with BNT162b2 and protection from symptomatic breakthrough infections in health care workers.
Clostridium difficile (CD) is a leading cause of diarrhoea among hospitalized patients. The objective this study was to evaluate the rate, optimal diagnostic work-up, and outcome CD infections (CDI) in Internal Medicine (IM) wards Italy.PRACTICE an observational prospective study, involving 40 IM Units evaluating all consecutive patients during 4-month period. CDI were defined case when both enzyme immunoassay for GDH, test A/B toxin positive. Patients with followed-up recurrences 4 weeks...
To complement RT-qPCR testing for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, many countries have introduced the use rapid antigen tests. As they generally display lower real-life performances than expected, their correct positioning as frontline screening is still controversial. Despite lack data from daily clinical use, third generation microfluidic assays (such LumiraDx SARS-CoV-2 Ag test) recently been suggested to similar and proposed...
Abstract Background To develop a pipeline for automatic extraction of quantitative metrics and radiomic features from lung computed tomography (CT) artificial intelligence (AI) models supporting differential diagnosis between coronavirus disease 2019 (COVID-19) other viral pneumonia (non-COVID-19). Methods Chest CT 1,031 patients (811 model building; 220 as independent validation set (IVS) with positive swab severe acute respiratory syndrome coronavirus-2 (647 COVID-19) or viruses (384...
Summary Objectives Rapid and reliable exclusion of invasive fungal infections (IFI) by markers able to avoid unnecessary empirical antifungal treatment is still a critical unmet clinical need. We investigated the diagnostic performance newly available β ‐ d ‐Glucan (BDG) quantification assay, focusing on optimisation BDG cut‐off values for IFI exclusion. Methods results Wako β‐glucan assay (lower limit detection [LLOD] = 2.16 pg/mL, positivity ≥ 11 pg/mL) two consecutive serum samples were...
Detection of subgenomic (sg) SARS-CoV-2 RNAs are frequently used as a correlate viral infectiousness, but few data about correlation between sg load and viable virus available. Here, we defined concordance culture isolation E N sgRNA quantification by ddPCR assays in 51 nasopharyngeal swabs collected from positive hospitalized patients. Among the samples, 14 were culture-positive 37 negative. According to results, sensitivity specificity 100% 100%, 84% 86%, respectively. ROC analysis showed...
Objective To evaluate the mean increase of anti-S IgG antibody titer between basal, pre-booster level to assessed 14 days after booster dose BNT162b2. Patients and Methods The RENAISSANCE study is an observational, longitudinal, prospective, population-based study, conducted on healthcare workers Niguarda Hospital in Milan, Italy who received a BNT162b2 at least 180 their second or positivity for SARS-CoV-2 accepted take part study. was from January 1, 2021 through December 28, 2021....