A5046364988- Poxvirus research and outbreaks
- Bacillus and Francisella bacterial research
- Herpesvirus Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- COVID-19 Impact on Reproduction
- Biosensors and Analytical Detection
- Plant Virus Research Studies
- HIV/AIDS Research and Interventions
- Trypanosoma species research and implications
- Diabetes and associated disorders
- HIV/AIDS drug development and treatment
- Viral Infections and Vectors
- Long-Term Effects of COVID-19
- HIV Research and Treatment
- Virology and Viral Diseases
- Research on Leishmaniasis Studies
- COVID-19 epidemiological studies
- Animal Virus Infections Studies
- COVID-19 diagnosis using AI
- Viral Infections and Outbreaks Research
- Advanced Biosensing Techniques and Applications
- Rabies epidemiology and control
ASST Fatebenefratelli Sacco
2018-2025
Luigi Sacco Hospital
2013-2025
National Research Council
2024
National Research Council
2024
Istituto di Scienze e Tecnologie Chimiche "Giulio Natta"
2024
University of Milan
2013-2022
Diagnostic Services Manitoba
2022
University of Pavia
2020
Vivantes Klinikum
2017
Charité - Universitätsmedizin Berlin
2017
Abstract The impact of SARS-CoV-2 infection during gestation remains unclear. Here, we analyse the viral genome on maternal and newborns nasopharyngeal swabs, vaginal umbilical cord plasma, placenta biopsies, amniotic fluids milk from 31 mothers with infection. In addition, also test specific anti-SARS-CoV-2 antibodies expression genes involved in inflammatory responses placentas, plasma. We detect one blood two at-term mucosa specimen. Furthermore, report presence IgM IgG Finally, three...
Introduction: COVID-19 Ag Respi-Strip, an immunochromatographic (ICT) assay for the rapid detection of SARS-CoV-2 antigen on nasopharyngeal specimen, has been developed to identify positive patients allowing prompt clinical and quarantine decisions. In this original research article, we describe conception, analytical performances as well risk management implementing Respi-Strip in a diagnostic decision algorithm. Materials Methods: Development resulted ready-to-use ICT based membrane...
Tocilizumab, a humanized monoclonal antibody, targets IL-6 receptors blocking downstream pro-inflammatory effects of IL-6. In preliminary reports it was suggested to be beneficial in patients with severe COVID-19.In this open-label prospective study we describe clinical characteristics and outcome 51 hospitalized confirmed COVID-19 pneumonia treated tocilizumab intravenously. All had elevated plasma level (>40 pg/mL) oxygen saturation <93% ambient air. Clinical outcomes, support, laboratory...
Wastewater-based epidemiology has been proposed to monitor the diffusion and trend of SARS-CoV-2 pandemic. In present study, raw treated samples from three wastewater treatment plants, two river characterized Milano Metropolitan Area, Italy, were surveyed for RNA positivity real time PCR infectiveness. Moreover, whole genome sequencing phylogenetic analysis isolated strains was performed. Raw resulted positive amplification, while water always negative (four samples, respectively, sampled in...
Anal and urethral samples from confirmed cases of monkeypox were screened for virus (MPXV) by real-time PCR. Isolation the was subsequently attempted in cell culture. Actively-replicating demonstrated 13 18 11 15 PCR-positive anal swabs, respectively, collected within 7 days symptoms onset. Two asymptomatic secondary had detectable MPXV genetic material secretion one, successfully isolated, supporting a potential sexual transmission hypothesis.
Monkeypox virus (mpxv) started to spread Europe and North America at the beginning of current outbreak in May 2022, World Health Organization (WHO) declared Human (mpox) as a public health emergency international concern (PHEIC) July 2022. The aim this observational analysis is describe demographical data, symptoms presentation clinical course till outcome individuals diagnosed with mpox, between October our open-access Sexual Clinic IRCCS San Raffaele Hospital Milan, Italy.Among people who...
Patients with type 1 diabetes (T1D) may develop severe outcomes during coronavirus disease 2019 (COVID-19), but their ability to generate an immune response against the SARS-CoV-2 mRNA vaccines remains be established. We evaluated safety, immunogenicity, and glycometabolic effects of acute respiratory syndrome 2 (SARS-CoV-2) in patients T1D. A total 375 (326 T1D 49 subjects without diabetes) who received two doses (mRNA-1273, BNT162b2) between March April 2021 at ASST Fatebenefratelli Sacco...
As the SARS-CoV-2 pandemic continues to rage worldwide, emergence of numerous variants concern (VOC) represents a challenge for vaccinal protective efficacy and reliability commercially available high-throughput immunoassays. Our study demonstrates administration two doses BNT162b2 vaccine that elicited robust SARS-CoV-2-specific immune response which was assessed up 3 months after full vaccination in cohort 37 health care workers (HCWs). antibody response, evaluated by four...
Monkeypox virus (MPXV) infection confirmation needs reliable polymerase chain reaction (PCR) assays; in addition, viral clade attribution is a key factor containment measures, considering more severe syndrome I and possibility of simultaneous circulation. This study aims to evaluate the performance all-in-one STANDARD M10 MPX/OPX (SD BIOSENSOR, South Korea – M10). Frozen samples from 205 subjects were selected stratified according routine test results (RealStar® Orthopoxvirus PCR Kit 1.0,...
Chandipura virus (CHPV) is an arthropod-borne linked to encephalitis in humans, primarily India. Its evolutionary dynamics and transmission pathways remain poorly understood due limited genomic data. This study analyzed 23 publicly available CHPV genomes, including isolates from sandflies, a hedgehog, retrieved GenBank. Phylogenetic analyses were conducted explore host-specific geographic patterns. analysis revealed distinct lineages. Human-derived genomes collected India between 2003 2024...
A diluted 3% w/w hydrogen peroxide solution acidified to pH 2.5 by adding citric acid inactivated SARS-CoV-2 virus more than 4 orders of magnitude in 5 min. After a contact time 15 min, no viral replication was detected. Aqueous solutions sodium percarbonate coronavirus >3 log10 diminution Conversely, H2O2 with additives displayed scarce virucidal activity (1.1 min), confirming that pH-modifying ingredient is necessary have H2O2-based disinfectant active against the novel coronavirus.