A5059408290- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- SARS-CoV-2 and COVID-19 Research
- HIV/AIDS Research and Interventions
- Hepatitis C virus research
- Hepatitis B Virus Studies
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Liver Disease Diagnosis and Treatment
- Antibiotic Resistance in Bacteria
- Respiratory viral infections research
- Viral Infections and Outbreaks Research
- Immunotherapy and Immune Responses
- Vibrio bacteria research studies
- COVID-19 diagnosis using AI
- Virology and Viral Diseases
- HIV-related health complications and treatments
- T-cell and B-cell Immunology
- Antibiotics Pharmacokinetics and Efficacy
- COVID-19 epidemiological studies
- Biochemical and Molecular Research
- vaccines and immunoinformatics approaches
- Streptococcal Infections and Treatments
- Pneumonia and Respiratory Infections
- Genomics and Phylogenetic Studies
University of Milan
2013-2025
Bambino Gesù Children's Hospital
2021-2025
Ospedale Maggiore
2025
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2024-2025
European Institute of Oncology
2021-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2021-2023
Centre International De Reference Chantal Biya
2023
Mylan (Switzerland)
2022
Ospedale San Paolo
2021
Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda
2021
Abstract From February to April 2020, Lombardy (Italy) reported the highest numbers of SARS-CoV-2 cases worldwide. By analyzing 346 whole genomes, we demonstrate presence seven viral lineages in Lombardy, frequently sustained by local transmission chains and at least two likely have originated Italy. Six single nucleotide polymorphisms (five them non-synonymous) characterized sequences, none affecting N-glycosylation sites. The lineages, clusters within three them, revealed that community...
Breakthrough SARS-CoV-2 infections in fully vaccinated individuals are considered a consequence of waning immunity. Serum antibodies represent the most measurable outcome vaccine-induced B cell memory. When decline, memory cells expected to persist and perform their function, preventing clinical disease. We investigated whether BNT162b2 mRNA vaccine induces durable functional vivo against 3, 6, 9 months after second dose cohort health care workers (HCWs). While we observed physiological...
Hepatitis B virus (HBV) reactivation during immunosuppression can lead to severe acute hepatitis, fulminant liver failure, and death. Here, we investigated hepatitis surface antigen (HBsAg) genetic features underlying this phenomenon by analyzing 93 patients: 29 developing HBV 64 consecutive patients with chronic infection (as control). HBsAg diversity was analyzed population-based ultradeep sequencing (UDS). Before reactivation, 51.7% of were isolated core antibody (anti-HBc) positive,...
Since SARS-CoV-2-based disease (COVID-19) spreads as a pandemic, the necessity of highly sensitive molecular diagnosis that can drastically reduce false negatives reverse transcription PCR (rtPCR) results, raises major clinical need. Here we evaluated performance ddPCR-based assay to quantify SARS-CoV-2 titer in 55 suspected COVID-19 cases with negative rtPCR results thanks in-house ddPCR (targeting RdRp and host RNaseP). Samples were collected at ASST-GOM Niguarda between February May 2020...
Food additives, such as emulsifiers, stabilizers, or bulking agents, are present in the Western diet and their consumption is increasing. However, little known about potential effects on intestinal homeostasis. In this study we examined effect of some these food additives gut inflammation.Mice were given drinking water containing maltodextrin (MDX), propylene glycol, animal gelatin, then challenged with dextran sulfate sodium indomethacin. parallel, mice fed a MDX-enriched endoplasmic...
Specific memory B cells and antibodies are a reliable read-out of vaccine efficacy. We analysed these biomarkers after one two doses BNT162b2 vaccine. The second dose significantly increases the level highly specific antibodies. Two months dose, antibody levels decline, but continue to increase, thus predicting sustained protection from COVID-19. show that although mucosal IgA is not induced by vaccination, migrate in response inflammation secrete at sites. first may lead an insufficient...
Background Group A Streptococcus (GAS) causes multiple clinical manifestations, including invasive (iGAS) or even life-threatening (severe-iGAS) infections. After the drop in cases during COVID-19 pandemic, 2022 a sharp increase of GAS was reported globally. Methods strains collected 09/2022–03/2023 two university hospitals Milan, Italy were retrospectively analyzed. Clinical/epidemiological data combined with whole-genome sequencing to: (i) define resistome/virulome, (ii) identify putative...
Background. We evaluated reliability and clinical usefulness of genotypic resistance testing (GRT) in patients for whom combination antiretroviral therapy (cART) was unsuccessful with viremia levels 50–1000 copies/mL, GRT is generally not recommended by current guidelines. Methods. The genotyping success rate 12 828 human immunodeficiency virus type 1 (HIV-1) plasma samples >50 tested using the commercial ViroSeq HIV-1 Genotyping System or a homemade system. Phylogenetic analysis performed...
<h3>Importance</h3> Although several studies have provided information on short-term clinical outcomes in children with perinatal exposure to SARS-CoV-2, data the immune response first months of life among newborns exposed virus utero are lacking. <h3>Objective</h3> To characterize systemic and mucosal antibody production during 2 infants who were born mothers infected SARS-CoV-2. <h3>Design, Setting, Participants</h3> This prospective cohort study enrolled 28 pregnant women tested positive...
Abstract Objectives Genomic surveillance of Klebsiella pneumoniae carbapenemase-producing (KPC-Kp) is crucial for virulence, drug-resistance monitoring, and outbreak containment. Methods analysis on 87 KPC-Kp strains isolated from 3 Northern Italy hospitals in 2019-2021 was performed by whole genome sequencing (WGS), to characterize resistome, virulome, mobilome, assess potential associations with phenotype resistance clinical presentation. Maximum Likelihood Minimum Spanning Trees were used...
Abstract Background Recent studies showed that plasma SARS-CoV-2 RNA seems to be associated with worse COVID-19 outcome. However, whether specific population can at higher risk of viremia are date unexplored. Methods This cross-sectional proof-of-concept study included 41 SARS-CoV-2-positive adult individuals (six affected by haematological malignancies) hospitalized two major hospital in Milan, for those demographic, clinical and laboratory data were available. load was quantified ddPCR...
Since the start of SARS-CoV-2 pandemic, children aged ≤ 12 years have always been defined as underrepresented in terms infections' frequency and severity. By correlating transmission dynamics with clinical virological features 612 positive patients years, we demonstrated a sizeable circulation different lineages over four pandemic waves paediatric population, sustained by local chains. Age < 5 highest viral load, gamma delta clades positively influence this transmission. No correlations...
Abstract Little is known about SARS-CoV-2 evolution under Molnupiravir and Paxlovid, the only antivirals approved for COVID-19 treatment. By investigating variability in 8 Molnupiravir-treated, 7 Paxlovid-treated 5 drug-naïve individuals at 4 time-points (Days 0-2-5-7), a higher genetic distance found pressure compared to Paxlovid no-drug (nucleotide-substitutions/site mean±Standard error: 18.7 × 10 −4 ± 2.1 vs. 3.3 0.8 3.1 , P = 0.0003), peaking between Day 2 5. drives emergence of more G-A...
Background. This study characterizes and defines the clinical value of hepatitis B virus (HBV) quasispecies with reverse transcriptase HBV surface antigen (HBsAg) heterogeneity in patients acute infection. Methods. Sixty-two infection (44 genotype D 18 A infection) were enrolled from 2000 to 2010. Plasma samples obtained at time first examination analyzed by ultradeep pyrosequencing. The extent HBsAg amino acid variability was measured Shannon entropy. Results. Median alanine...
COVID-19 has significantly affected hospital infection prevention and control (IPC) practices, especially in intensive care units (ICUs). This frequently caused dissemination of multidrug-resistant organisms (MDROs), including carbapenem-resistant Acinetobacter baumannii (CRAB). Here, we report the management a CRAB outbreak large ICU hub Hospital Italy, together with retrospective genotypic analysis by whole-genome sequencing (WGS). Bacterial strains obtained from severe mechanically...
We tested whether pre-HAART viraemia affects the achievement and maintenance of virological success in HIV-1-infected patients starting modern first-line therapies.A total 1,430 their first HAART (genotype-tailored) 2008 (median; IQR: 2006-2009) were grouped according to levels (≤ 30,000, 30,001-100,000, 100,001-300,000, 300,001-500,000 > 500,000 copies/ml). The impact pre-therapy on time (viraemia ≤ 50 copies/ml) rebound (first two consecutive values copies/ml after success) evaluated by...
Despite effective HIV suppression, neuroinflammation and neurocognitive issues are prevalent in people with (PWH) yet poorly understood. infection alters the human virome, virome perturbations have been linked to without HIV. Once thought be sterile, cerebrospinal fluid (CSF) hosts a recently discovered presenting an unexplored avenue for understanding brain mental health PWH. This cross-sectional study analyzed 85 CSF samples (74 from PWH on suppressive antiretroviral therapy, 11 controls...
Prolonged SARS-CoV-2 infection observed in immunocompromised individuals even the presence of antiviral treatment provides opportunities for viruses to evolve immune escape and drug-resistant variants. A 72-year-old male with IgG4-related disease was admitted Emergency Department a city Hospital Milan then transferred Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico December 2023, due respiratory distress diagnosed November 2023. After 117 days since onset infection, two cycles...
With the advent of COVID-19, anti-SARS-CoV-2 vaccines were a global health priority, but evidence on their significance within tropical settings remained limited. We sought to assess distribution antibodies according vaccine status and types administered in Cameroon during Omicron waves. A community based cross-sectional sero-survey was conducted from February-15 through July-31 2022 among individuals tested for COVID-19 Yaoundé-Cameroon. Sociodemographic data collected participants....
This retrospective study analyzed SARS-CoV-2 Omicron variability since its emergence, focusing on immunocompromised (IPs) and non-immunocompromised adult people (NIPs). Phylogenetic analysis identified at least five major lineage groups circulating in Central Italy, from December 2021 to 2023: (a) BA.1 (34.0%), (b) BA.2 + BA.4 (25.8%), (c) BA.5 BF (10.8%), (d) BQ BE EF (9.2%), (e) Recombinants (20.2%). The lineages were more common IPs compared NIPs (30.9% vs. 17.8%, respectively; p =...