Luca Carioti
A5008531739- Hepatitis C virus research
- Hepatitis B Virus Studies
- HIV/AIDS drug development and treatment
- Liver Disease Diagnosis and Treatment
- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- HIV/AIDS Research and Interventions
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Biochemical and Molecular Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Viral Infections and Outbreaks Research
- Pneumonia and Respiratory Infections
- Systemic Lupus Erythematosus Research
- vaccines and immunoinformatics approaches
- Chronic Lymphocytic Leukemia Research
- Vibrio bacteria research studies
- Blood groups and transfusion
- Ruminant Nutrition and Digestive Physiology
- Bacteriophages and microbial interactions
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Genetic and phenotypic traits in livestock
- Long-Term Effects of COVID-19
- Viral gastroenteritis research and epidemiology
- COVID-19 and Mental Health
University of Rome Tor Vergata
2016-2025
Chinese University of Hong Kong
2022
University of Hong Kong
2022
St Vincent's Hospital
2013
Hepatitis B virus (HBV) reactivation during immunosuppression can lead to severe acute hepatitis, fulminant liver failure, and death. Here, we investigated hepatitis surface antigen (HBsAg) genetic features underlying this phenomenon by analyzing 93 patients: 29 developing HBV 64 consecutive patients with chronic infection (as control). HBsAg diversity was analyzed population-based ultradeep sequencing (UDS). Before reactivation, 51.7% of were isolated core antibody (anti-HBc) positive,...
The process of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genetic diversification is still ongoing and has very recently led to the emergence a new variant concern (VOC), defined as Omicron or B.1.1.529. VOC most divergent identified so far generated immediate for its potential capability increase SARS-CoV-2 transmissibility and, more worryingly, escape therapeutic vaccine-induced antibodies. Nevertheless, clear definition mutational spectrum missing. Herein, we provide...
Objective The involvement of HBV DNA integration in promoting hepatocarcinogenesis and the extent to which intrahepatic reservoir modulates liver disease progression remains poorly understood. We examined reservoir, occurrence its impact on hepatocyte transcriptome hepatitis B ‘e’ antigen (HBeAg)-negative chronic (CHB). Design Liver tissue from 84 HBeAg-negative patients with CHB low (n=12), moderate (n=25) high (n=47) serum was analysed. Covalently closed circular (cccDNA), pregenomic RNA...
Since the emergence of SARS-CoV-2, several recurrent mutations, particularly in spike protein, arose during human-to-human transmission or spillover events between humans and animals, generating distinct worrisome variants concern (VOCs) interest (VOIs), designated as such due to their clinical diagnostic impacts. Characterizing these related mutations is important tracking SAR-CoV-2 evolution understanding efficacy vaccines therapeutics based on monoclonal antibodies, convalescent-phase...
Background. This study characterizes and defines the clinical value of hepatitis B virus (HBV) quasispecies with reverse transcriptase HBV surface antigen (HBsAg) heterogeneity in patients acute infection. Methods. Sixty-two infection (44 genotype D 18 A infection) were enrolled from 2000 to 2010. Plasma samples obtained at time first examination analyzed by ultradeep pyrosequencing. The extent HBsAg amino acid variability was measured Shannon entropy. Results. Median alanine...
Specific HBsAg mutations are known to hamper recognition by neutralizing antibodies thus challenging HBV-vaccination efficacy. Nevertheless, information on their impact and spreading over time is limited. Here, we characterize the circulation of vaccine-escape from 2005 2019 correlation with virological parameters in a large cohort patients infected HBV genotype-D (N = 947), dominant Europe. Overall, 17.7% harbours ≥1 mutation highest prevalence subgenotype-D3. Notably, complex profiles...
Abstract Objectives We evaluated the HIV-1 capsid genetic variability and lenacapavir drug resistance-associated mutations (DRMs) among drug-naive individuals across clades. Methods A total of 2031 sequences from patients were analysed for amino acid modification prevalence DRMs. Amino positions with <5% considered as conserved was by Results Overall, 63% (148/232) in protein. Of note, conservation consistent specific binding residues cellular factors involved viral replication [CypA...
Next-generation sequencing (NGS) is gradually replacing Sanger for HIV genotypic drug resistance testing (GRT). This work evaluated the concordance among different NGS-GRT interpretation tools in a real-life setting.
ABSTRACT Since early 2022 the Omicron variant has rapidly spread worldwide, becoming dominant to date. The study aimed investigate clinical and epidemiological characteristics of COVID‐19 patients reconstruct genomic epidemiology main SARS‐CoV‐2 sublineages in Italy 2022. A total 8970 samples were studied, phylogenetic analyses focused on BA.1, BA.2, BA.5 subvariants. More than half subjects received three doses vaccine experienced a reinfection. significant larger proportion unvaccinated...
This retrospective study analyzed SARS-CoV-2 Omicron variability since its emergence, focusing on immunocompromised (IPs) and non-immunocompromised adult people (NIPs). Phylogenetic analysis identified at least five major lineage groups circulating in Central Italy, from December 2021 to 2023: (a) BA.1 (34.0%), (b) BA.2 + BA.4 (25.8%), (c) BA.5 BF (10.8%), (d) BQ BE EF (9.2%), (e) Recombinants (20.2%). The lineages were more common IPs compared NIPs (30.9% vs. 17.8%, respectively; p =...
This study investigated the role of low-frequency INSTI resistance mutations, detectable by next-generation sequencing (NGS), in predicting virological rebound (VR) among people with HIV (PWH) starting second-generation INSTI-based first-line regimens. case-control compared PWH (retrieved from Icona cohort; www.icona.org) who experienced VR (cases) those maintained control (controls) under regimens based on dolutegravir or bictegravir. NGS data obtained through Illumina platform were...
Post COVID-19 condition (PCC) affects 10-40% of patients and is characterized by persisting symptoms at ≥ 4 weeks after SARS-CoV-2 infection. Symptoms can last 7 or even more months. How long PCC persists any changes in its clinical phenotypes over time require further investigation. We investigated trajectories factors associated with persistence. included both hospitalized outpatients from February 2020 to June 2023, who underwent least one follow-up visit acute infection San Paolo...
Herein, we report a case of an Italian male infected by Delta sublineage AY.4 harboring atypical deletion, leading to N gene target failure (NGTF) commercial molecular assay for SARS-CoV-2 diagnosis (AllplexTM Assay, Seegene). A 59-year-old unvaccinated patient was hospitalized pulmonary embolism, with first negative results obtained both and antigen tests. After several days viral negativity, he presented positive E RdRP/S genes, but in gene. Negativity repeatedly confirmed the following...
SARS-CoV-2 is constantly evolving, leading to new variants. We analysed data from 4400 SARS-CoV-2-positive samples in order pursue epidemiological variant surveillance and evaluate their impact on public health Italy the period of April-December 2021. The main circulating strain (76.2%) was Delta variant, followed by Alpha (13.3%), Omicron (5.3%), Gamma variants (2.9%). B.1.1 lineages, Eta, Beta, Iota, Mu, Kappa variants, represented around 1% cases. There were 48.2% subjects who had not...
To clarify whether next-generation sequencing (NGS) can be useful for resistance assessment in virologically suppressed highly treatment-experienced (HTE) individuals with MDR HIV.
Transmitted drug resistance (TDR) is still a critical aspect for the management of individuals living with HIV-1. Thus, its evaluation crucial to optimize HIV care.
Objective We evaluated the characteristics of HIV-1 molecular transmission clusters (MTCs) in 1890 newly diagnosed individuals infected with non-B subtypes between 2005 and 2017 Italy. Methods Phylogenetic analyses were performed on pol sequences to characterise subtypes/circulating recombinant forms identify MTCs. MTCs divided into small (SMTCs, 2–3 sequences), medium (MMTCs, 4–9 sequences) large (LMTCs, ≥10 sequences). Factors associated using logistic regression analysis. Results 145...
We evaluated natural resistance to the new antiretroviral fostemsavir and its potential association with other HIV-1 gp120 polymorphisms.A total of 1997 B subtype sequences from Los Alamos HIV Database were analysed for mutation prevalence at resistance-associated positions polymorphisms. The role each resistance-related position correlated mutations, both in protein stability reducing binding affinity between antibody and/or T cell lymphocyte epitopes MHC molecules, was estimated.The...
Transmitted drug-resistance (TDR) remains a critical aspect for the management of HIV-1-infected individuals. Thus, studying dynamics TDR is crucial to optimize HIV care. In total, 4323 HIV-1 protease/reverse-transcriptase sequences from drug-naive individuals diagnosed in north and central Italy between 2000 2014 were analysed. was evaluated over time. Maximum-likelihood Bayesian phylogenetic trees with bootstrap Bayesian-probability supports defined transmission clusters. Most males...
With limited and low-genetic barrier drugs used for the prevention of mother-to-child transmission (PMTCT) HIV in sub-Saharan Africa, vertically transmitted HIV-1 drug-resistance (HIVDR) is concerning might prompt optimal pediatric strategies. The aim this study was to ascertain HIVDR viral-tropism majority minority populations among Cameroonian infected children. A comparative analysis 18 HIV-infected children (7 from PMTCT-exposed mothers 11 without PMTCT-exposure) performed. co-receptor...
Background: The aim of the study was to investigate intra-host variability through next-generation-sequencing (NGS) NS5A-gene in nosocomial transmission-clusters observed two Italian hospitals among hepatitis C virus (HCV)-genotype-1b infected patients. Methods: HCV-sequencing performed by Sanger-sequencing (NS3 + NS5A NS5B) and NGS (NS5A, MiSeq-Illumina) 15 HCV-1b patients [five acute with onco-hematologic-disease 10 (4/6 acute/chronic) β-thalassemia]. Resistance-associated-substitutions...
ABSTRACT Incomplete APOBEC3G/F neutralization by a defective HIV-1Vif protein can promote genetic diversification inducing G-to-A mutations in the HIV-1 genome. The Env V3 loop, critical for coreceptor usage, contains several putative target sites. Here, we determined if APOBEC3G/F, presence of Vif-defective virus, induce at positions to modulation CXCR4 usage. Peripheral blood mononuclear cells (PBMC) and monocyte-derived macrophages (MDM) from 2 HIV-1-negative donors were infected with...