A5021487791- Streptococcal Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- SARS-CoV-2 and COVID-19 Research
- Infective Endocarditis Diagnosis and Management
- Enterobacteriaceae and Cronobacter Research
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Pneumonia and Respiratory Infections
- T-cell and B-cell Immunology
- Diphtheria, Corynebacterium, and Tetanus
- Immunotherapy and Immune Responses
- Sepsis Diagnosis and Treatment
- Long-Term Effects of COVID-19
- Orthopedic Infections and Treatments
- Mycotoxins in Agriculture and Food
- Respiratory Support and Mechanisms
- Evolution and Genetic Dynamics
- Yeasts and Rust Fungi Studies
- Health and Lifestyle Studies
- Neonatal Respiratory Health Research
- Nosocomial Infections in ICU
- Bacterial Infections and Vaccines
- Neonatal and Maternal Infections
- Psychological Well-being and Life Satisfaction
Bambino Gesù Children's Hospital
2021-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2021-2025
Specific memory B cells and antibodies are a reliable read-out of vaccine efficacy. We analysed these biomarkers after one two doses BNT162b2 vaccine. The second dose significantly increases the level highly specific antibodies. Two months dose, antibody levels decline, but continue to increase, thus predicting sustained protection from COVID-19. show that although mucosal IgA is not induced by vaccination, migrate in response inflammation secrete at sites. first may lead an insufficient...
Children are prone to bloodstream infections (BSIs), the rapid and accurate diagnosis of which is an unmet clinical need. The T2MR technology a direct molecular assay for identification BSI pathogens, can help overcome limits blood culture (BC) such as diagnostic accuracy, volumes required, turnaround time. We analyzed results obtained with T2Bacteria (648) T2Candida (106) panels in pediatric patients Bambino Gesù Children's Hospital between May 2018 September 2020 order evaluate performance...
Background: Bloodstream infections affect up to 20% of pediatric cancer patients receiving intensive care, contributing significantly morbidity and mortality, with infection-related mortality rates reported be as high 16%. Methods: The identification microorganisms directly from whole blood is difficult due several factors, such interference host genomic material, low bacterial load, the endogenous components exogenous substances, which can interfere process. Nevertheless, rapid microbial...
Background/Objectives: Paediatric osteoarticular infections (POAIs) present unique diagnostic and therapeutic challenges. Microbiological culture (MC) is typically time-consuming lacks sensitivity, especially when patients have received antibiotics. The BIOFIRE® Joint Infection Panel (BJIP) a syndromic molecular assay for the direct identification of most pathogens causing POAIs. Methods: We evaluated BJIP in 17 synovial fluids, then, we retrospectively assessed its utility 93 off-label...
Owing to an increasing number of infections in adults, Lactococcus (L.) garvieae has gained recognition as emerging human pathogen, causing bacteraemia and septicaemia. In September 2020, four paediatric onco-hematologic patients received a platelet concentrate from the same adult donor at Bambino Gesù Children's Hospital IRCCS, Rome. Three experienced L. sepsis one day after transfusion. The pediatric isolates donor's concentrates were retrospectively collected for whole-genome sequencing...
Invasive group A streptococci infections are increasing worldwide, mainly due to the emm1 lineage M1 UK emergence. Although this variant has recently been described in adult Italian patients, its circulation children not yet established. Characterizing by whole genome sequencing 6 invasive strains isolated between 2022 and 2023, we highlighted pediatric patients Italy.
Cystic fibrosis (CF) is a life-threatening genetic disease characterised by chronic lung infections sustained opportunistic pathogens such as
Background: Infant meningitis, particularly caused by Escherichia coli, remains a life-threatening condition, especially in premature and low-weight infants. Infections of the central nervous system can be fatal, necessitating prompt diagnosis appropriate treatment. Acute infections various pathogens, including E. often present with similar clinical symptoms. The rapid identification pathogens their antimicrobial resistance mechanisms is critical for timely effective We report case an...
Abstract Specific memory B cells and antibodies are reliable read-out of vaccine efficacy. We analyzed these biomarkers after one two doses BNT162b2 vaccine. The second dose significantly increases the level highly-specific antibodies. Two months dose, specific antibody levels decline, but highly continue to increase thus predicting a sustained protection from COVID-19. Graphical
Trichosporon japonicum is a very rare opportunistic yeast causing fungal disease in humans, especially immunocompromised hosts. Here, we describe new case of T. isolated from the blood pyrexial pediatric patient with refractory acute B cell lymphoblastic leukemia and respiratory distress. Prompt diagnosis through early clinical suspicion appropriate molecular microbiology analysis allowed to be accurately identified at species level. Subsequent drug susceptibility testing focused antifungal...
The spread of carbapenemase-producing Enterobacterales (CPE), especially Klebsiella pneumoniae (K. pneumoniae) and Escherichia coli (E. coli), is a serious public health threat in pediatric hospitals. associated risk newborns due to their underdeveloped immune system limited treatment options. aim was estimate the prevalence circulation CPE among neonatal intensive units major hospital Italy investigate molecular features. A total 124 were isolated from rectal swabs 99 newborn patients at...
Due to the global spread of pan resistant organisms, colistin is actually considered as one last resort antibiotics against MDR and XDR bacterial infections. The emergence strains has been observed worldwide in Gram-negative bacteria, such Enterobacteriaceae especially K. pneumoniae, association with increased morbidity mortality. This landscape implies exploration novel assays able target rapidly. In this study, we developed evaluated a new MALDI-TOF MS assay positive-ion mode that allows...
This article reports a rapid and unexpected spread of colonization cases NDM-1 carbapenemase-producing Klebsiella pneumoniae Escherichia coli in neonatal surgical unit (NSU) at Bambino Gesù Children's Hospital Rome, Italy. Between the 16th November 2020 18th January 2021, total 20 K. (n = 8) E. 12) were isolated from 17 out 230 stool samples collected neonates admitted aforementioned ward time period by an active surveillance culture program routinely place to monitor prevalence...
Bacterial infections caused by multidrug-resistant (MDR) Gram-negatives are of great concern worldwide, as they frequently associated with high mortality and morbidity rates. To date, two cases VIM-2 metallo-β-lactamase (MBL)-producing Pseudomonas putida bacteremia have been ever reported in France Spain between 2004 2010. Here, we present the first case VIM-1-like-producing P. isolated blood culture collected from an oncohematological pediatric patient admitted to Bambino Gesù Children's...
Blood level of pancreatic stone protein (PSP) is a promising biomarker sepsis both in adults and children. The aim our study was to investigate the diagnostic accuracy PSP children with suspected compare performance other biomarkers approved for clinical use, that is, procalcitonin (PCT) C-reactive (CRP).
(1) Background: The literature reports a low risk of serious bacterial infections (SBIs) in febrile infants presenting with bronchiolitis or respiratory syncytial virus infection, but current microbiological techniques have higher accuracy. (2) Methods: We assessed the SBIs neonates and from 2021 to 2023. also evaluated C-reactive protein, procalcitonin, leukocyte values. (3) Results: included 242 infants. Blood cultures (BCs) were performed 66/242 patients, positivity rate 9.1% (including one BC
Abstract Background The COVID-19 outbreak has forcibly overshadowed the physical well-being of children and adolescents, which we will see consequences in near future. programs to contain spread Sars-CoV-2 resulted prolonged lockdown periods, discontinuity educational services a possible decrease activities (PA) among youngest. In local reality Metropolitan City Bologna adolescents underwent radical change habits lifestyle, overall predisposing sedentariness unhealthy behaviors. Our project,...