A5003642102- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Immune Response and Inflammation
- Influenza Virus Research Studies
- Infective Endocarditis Diagnosis and Management
- Respiratory viral infections research
- Virology and Viral Diseases
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Amoebic Infections and Treatments
- Microbial infections and disease research
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Reproductive tract infections research
- Cellular transport and secretion
- Streptococcal Infections and Treatments
- Genomics and Phylogenetic Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Tuberculosis Research and Epidemiology
- Complement system in diseases
- Escherichia coli research studies
- Protist diversity and phylogeny
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Erythrocyte Function and Pathophysiology
Institut Pasteur
2016-2025
Université Paris Cité
2015-2025
Université Paris-Saclay
2023
Centers for Disease Control and Prevention
2016
Centre de Recherche Médicale et Sanitaire
2016
Agence de Médecine Préventive
2016
World Health Organization
2016
Norwegian Institute of Public Health
2016
Délégation Paris 5
2015
Sorbonne Paris Cité
2015
BackgroundThe Invasive Respiratory Infection Surveillance (IRIS) Consortium was established to assess the impact of COVID-19 pandemic on invasive diseases caused by Streptococcus pneumoniae, Haemophilus influenzae, Neisseria meningitidis, and agalactiae. We aimed analyse incidence distribution these during first 2 years compared preceding pandemic.MethodsFor this prospective analysis, laboratories in 30 countries territories representing five continents submitted surveillance data from Jan...
Recombinant forms of Neisseria meningitidis human factor H binding protein (fHBP) are undergoing clinical trials in candidate vaccines against invasive meningococcal serogroup B disease. We report an extensive survey and phylogenetic analysis the diversity fhbp genes predicted sequences isolates obtained period 2000-2006.Nucleotide were from 1837 N. (MnB) strains United States, Europe, New Zealand, South Africa. Multilocus sequence typing (MLST) was performed on a subset strains.Every strain...
Innate lymphoid cells (ILCs) are involved in immune responses to microbes and various stressed cells, such as tumor cells. They include group 1 [such natural killer (NK) ILC1], 2, 3 ILCs. Besides their capacity respond cytokines, ILCs detect targets through a series of cell surface-activating receptors recognizing microbial nonmicrobial ligands. The nature some these ligands remains unclear, limiting our understanding ILC biology. We focused on NKp46, which is highly conserved mammals...
Summary The initial attachment of Neisseria meningitidis to the target cell surface appears be largely pilus depend‐ent in capsulated bacteria. Intimate adhesion subsequently occurs permit colonization. We recently reported that insertional inactivation crgA gene, which encodes a transcriptional regulator belonging LysR family, decreased meningococcal epithelial cells and abolished intimate adhesion. In this report, we analyse expression pilE sia genes, are involved biosynthesis pili capsule...
Meningococci spread via respiratory droplets, whereas the closely related gonococci are transmitted sexually. Several outbreaks of invasive meningococcal disease have been reported in Europe and United States among men who sex with (MSM). We recently identified an outbreak serogroup C MSM Germany France. In this study, genomic proteomic techniques were used to analyze isolates. addition, genetically identical urethritis isolates recovered from France included analysis. Genome sequencing...
ABSTRACT Bivalent rLP2086 (Trumenba), a vaccine for prevention of Neisseria meningitidis serogroup B (NmB) disease, was licensed use in adolescents and young adults after it demonstrated that elicits antibodies initiate complement-mediated killing invasive NmB isolates serum bactericidal assay with human complement (hSBA). The consists two factor H binding proteins (fHBPs) representing divergent subfamilies to ensure broad coverage. Although is the surrogate efficacy, an hSBA not suitable...
Abstract Objective Few data are available on the association between SARS-CoV-2 and secondary bacterial infections. Such an was described for flu invasive meningococcal disease (IMD). We aimed exploring such a correlation COVID-19 IMD as well impact of lockdown IMD. Results compared cases received at French National Reference Centre meningococci Haemophilus influenzae that sent part mandatory reporting these during period 01 January-15 May 2020 to those from same in 2018 2019. were...
BackgroundSince the appearance of COVID-19 in January 2020, invasive bacterial infections have decreased significantly worldwide. However, alterations age and sex distributions, clinical forms, phenotypes, genotypes isolates not been analyzed. Our goal is to present discuss these data considering current pandemic situation. Methods: The national reference center for meningococci Haemophilus influenzae France were mined examine above aspects infection before (2018−2019) after (2020−2021)...
Invasive meningococcal disease (IMD) cases declined upon the implementation of non-pharmaceutical measures to control COVID-19 pandemic. A rebound in IMD was feared easing these measures.We conducted a retrospective descriptive study using French National Reference Center Database for meningococci between 2015 and 2022. We scored serogroups, sex, age groups, clonal complexes corresponding isolates.Our data clearly show decline number all serogroups groups until 2021. This mainly due decrease...
Abstract We have previously shown that macrophage infection with Mycobacterium tuberculosis and M. bovis bacillus Calmette-Guérin (BCG) partially inhibits MHC class II surface expression in response to IFN-γ. The present study examined the nature of molecules do fact reach infected cells. Immunostaining specific Abs discriminate between mature immature populations showed a predominance invariant chain (Ii)-associated at BCG-infected cells suggesting mycobacteria specifically block export...
Phagosomes containing M. tuberculosis and bovis BCG interact normally with early endosomes but fail to fuse late lysosomes. Whereas many events of mycobacterial phagosomes have been elucidated, the exact mechanism inhibition fusion lysosomes is still unclear. Several Rab GTPase proteins were shown be involved in membrane vesicular transport. In particular, Rab7 associates phagosomal regulates between This function was mediated epithelial cell models by effector RILP (Rab7-interacting...
Mycobacterium tuberculosis evades the innate antimicrobial defenses of macrophages by inhibiting maturation its phagosome to a bactericidal phagolysosome. Despite intense studies mycobacterial phagosome, mechanism persistence dependent on prolonged phagosomal retention coat protein coronin-1 is still unclear. The present study demonstrated that several proteins traffic intracellularly in M. bovis BCG-infected cells and one them, with an apparent subunit size M(r) 50,000, actively retains...
In 2015, Niger reported the largest epidemic of Neisseria meningitidis serogroup C (NmC) meningitis in sub-Saharan Africa. The NmC coincided with W (NmW) cases during season, resulting a total 9,367 meningococcal through June 2015. To clarify phylogenetic association, genetic evolution, and antibiotic determinants strains Niger, we sequenced genomes 102 isolates from this epidemic, comprising 81 21 NmW isolates. 82 were completed, all included analysis. All had sequence type 10217, which...
Reduced susceptibility to penicillin G in Neisseria meningitidis is mainly due alterations PBP2 encoded by the penA gene. However, this phenotype was not associated with reduced third-generation cephalosporins (C3Gs). We aimed study emergence of meningococci C3Gs (MIC >0.06 mg/L) France since 2012.Invasive meningococcal isolates were typed MLST and sequencing their antibiotic tested.Isolates represented 2% all invasive from 2012-15, but absent before. They harboured a new allele, penA327,...
Abstract Background Invasive meningococcal disease (IMD) cases declined upon the implementation of non-pharmaceutical interventions (NPI) (social distancing and mask wearing) to control COVID-19 pandemic but rebounded in 2022 numbers with genotypical changes strains. We explored here associated modifications clinical presentations IMD. Methods conducted a retrospective descriptive study using Database French National Reference Centre for meningococci Haemophilus influnezae IMD between 2015...
Invasive meningococcal disease (IMD), caused by infection with the bacterium Neisseria meningitidis, usually manifests as meningitis or septicemia and can be severe life-threatening (1). Six serogroups (A, B, C, W, X, Y) account for most cases (2). N. meningitidis is transmitted person-to-person via respiratory droplets oropharyngeal secretions. Asymptomatic persons carry transmit bacteria to others, potentially causing illness among susceptible persons. Outbreaks occur in conjunction large...
Haemophilus ducreyi, traditionally recognised as the etiological agent of chancroid, a genital ulcer disease, is increasingly being identified significant cause cutaneous ulcers in yaws-endemic regions across South Pacific, Southeast Asia, and Sub-Saharan Africa. Despite its clinical relevance, this pathogen remains poorly characterized, comprehensive genetic tools for analysing isolate relationships are still lacking. In study, we present follow-up our previous research developed multilocus...
Abdominal symptoms are increasingly reported in invasive meningococcal disease (IMD), but the underlying mechanisms remain unclear. We aimed to explore pathophysiology of these presentations using an animal model. utilized a collection 20 isolates that were either associated or not with abdominal presentations, which injected intraperitoneally into transgenic mice expressing human transferrin. employed histological examination, RNA sequencing (RNAseq) transcriptomic analysis, and reverse...
Serum bactericidal assay (SBA) is a functional that evaluates infection- and vaccine-induced neutralizing antibodies representing the serological correlate of protection against Neisseria meningitidis. However, it time consuming due to its readout using enumeration colony-forming units (CFUs), making this conventional SBA (C-SBA) difficult for large-scale use. We developed new method takes advantage bioluminescence N. meningitidis serogroup B (BioLux-SBA). The development steps involved...
Invasive meningococcal disease (IMD) of serogroup B is preventable by protein-based vaccines targeting one (Bivalent rLP2086 vaccine) or several variable proteins (4CMenB at the bacterial surface. The 4CMenB was licensed in Europe 2013 but has been recommended and reimbursed France for infants over 2 months old since April 2022. bivalent vaccine 2017 subjects 10 years older. Evaluating strain coverage fluctuations prior to large scale use highly informative. We analysed invasive isolates...