A5051160328- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Antibiotic Resistance in Bacteria
- Infective Endocarditis Diagnosis and Management
- Amoebic Infections and Treatments
- Reproductive tract infections research
- Streptococcal Infections and Treatments
- Virology and Viral Diseases
- Influenza Virus Research Studies
- Microbial infections and disease research
- Pneumocystis jirovecii pneumonia detection and treatment
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Immunology Research
- Enterobacteriaceae and Cronobacter Research
- Bacteriophages and microbial interactions
- Bacillus and Francisella bacterial research
- Cytomegalovirus and herpesvirus research
- Viral gastroenteritis research and epidemiology
- Parasitic infections in humans and animals
- Respiratory and Cough-Related Research
- Herpesvirus Infections and Treatments
- Ear Surgery and Otitis Media
- RNA and protein synthesis mechanisms
- Antimicrobial Resistance in Staphylococcus
University of West Attica
2019-2025
National School of Public Health
2010-2019
Meningitis Now
2014
National Public Health Organization
2005
Pasteur Hellenic Institute
1988-1994
BackgroundThe Invasive Respiratory Infection Surveillance (IRIS) Consortium was established to assess the impact of COVID-19 pandemic on invasive diseases caused by Streptococcus pneumoniae, Haemophilus influenzae, Neisseria meningitidis, and agalactiae. We aimed analyse incidence distribution these during first 2 years compared preceding pandemic.MethodsFor this prospective analysis, laboratories in 30 countries territories representing five continents submitted surveillance data from Jan...
ABSTRACT The distribution of serogroups and multilocus sequence types (STs) in collections disease-associated carried meningococci from the period 1991 to 2000 three European countries (the Czech Republic, Greece, Norway) was investigated. A total 314 patient isolates 353 asymptomatic carriers were characterized. frequency distributions clone complexes differed among between disease carrier isolate collections. Highly significant differentiation seen at each housekeeping locus. marked...
Invasive meningococcal disease (IMD) is a major cause of bacterial meningitis and septicaemia although infection by some serogroups may be prevented through vaccination. We aimed to describe the epidemiology IMD in EU/EEA countries during 2004-2014 monitor serogroup- age-specific trends, compare country trends period C conjugate (MCC) vaccine introduction.We analysed surveillance data age, gender, serogroup, outcome. estimated percentage change annual notification rate (NR), using linear...
The Meningococcal Antigen Typing System (MATS) was developed to identify meningococcus group B strains with a high likelihood of being covered by the 4CMenB vaccine, but is limited requirement for viable isolates from culture-confirmed cases. We examined if antigen genotyping could complement MATS in predicting strain coverage vaccine. From panel 3912 MATS-typed invasive meningococcal disease collected England and Wales 2007–2008, 2014–2015 2015–2016, 16 other countries 2000–2015, 3481 were...
Background The total incidence of invasive meningococcal disease (IMD) in Europe has been declining recent years; however, a rising due to serogroup W (MenW), predominantly sequence type 11 (ST-11), clonal complex (cc11), was reported some European countries. Aim aim this study compile the most laboratory surveillance data on MenW IMD from several countries assess trends Europe. Methods In observational, retrospective study, collected 2013–17 by national reference laboratories and units 13...
Clinical isolates of Neisseria meningitidis with reduced susceptibility to penicillin G (intermediate isolates, Pen(I)) harbor alterations in the penA gene encoding binding protein 2 (PBP2). A 402-bp DNA fragment 3' half was sequenced from a collection 1,670 meningococcal clinical 22 countries that spanned 60 years. Phenotyping, genotyping, and determination MICs were also performed. total 139 different alleles detected 38 highly related, clustered together maximum-likelihood analysis...
ABSTRACT A sensitive multiplex PCR assay for single-tube amplification that detects simultaneous herpes simplex virus type 1 (HSV-1), 2 (HSV-2), varicella-zoster (VZV), human cytomegalovirus (CMV), and Epstein-Barr (EBV) is reported with particular emphasis on how the method was optimized carried out its sensitivity compared to previously described assays. The has been used a limited number of clinical samples must be thoroughly evaluated in context. total 86 cerebrospinal fluid (CSF)...
ABSTRACT Twenty clinical samples (18 cerebrospinal fluid and 2 articular samples) were sent to 11 meningococcus reference centers located in different countries. Ten of these laboratories are participating the EU-MenNet program (a European Union-funded program) members Monitoring Group on Meningococci. The remaining laboratory was Burkina Faso. Neisseria meningitidis sought by detecting several meningococcus-specific genes ( crgA , ctrA 16S rRNA, porA ). PCR-based nonculture method for...
Neisseria meningitidis or meningococcus is divided into 12 distinct serogroups of which A, B, C, W, X, and Y are medically most important cause health problems in different parts the world. The epidemiology N. unpredictable over time across geographic regions. Globally, serogroup A has been prevalent African "meningitis belt" whereas B C have predominated Europe. In a paper published earlier this journal (1) , an increase invasive meningococcal disease (IMD) some European countries was...
Widespread use of pneumococcal conjugate vaccines (PCVs) has reduced vaccine-type invasive disease (IPD). We describe the serotype distribution IPD after extensive ten-valent PCV (PCV10; Synflorix, GSK) and 13-valent (PCV13; Prevenar 13, Pfizer) globally. data were obtained from surveillance sites participating in WHO-commissioned Pneumococcal Serotype Replacement Distribution Estimation (PSERENADE) project that exclusively used PCV10 or PCV13 (hereafter sites, respectively) their national...
During 2012, Streptococcus pneumoniae serotype 3 was identified by polymerase chain reaction in 15 out of 20 (75%) pleural fluid specimens from children with pneumococcal pneumonia complicated parapneumonic effusion Greece. One-third these had been immunized the 13-valent conjugated vaccine after age 12 months, according to national immunization schedule.
Serogroup B meningococcal (MenB) isolates currently account for approximately 90% of invasive disease (IMD) in Greece with ST-162 clonal complex predominating. The potential a multicomponent vaccine (4CMenB) recently licensed Europe was investigated order to find whether the aforementioned will cover MenB strains circulating Greece. A panel 148 serogroup characterized by multilocus sequence typing (MLST) and PorA subtyping. Vaccine components were typed sequencing factor H-binding protein...
Invasive meningococcal disease (IMD) remains a major public health challenge due to its rapid progression, which may lead severe sequelae or death in children and adolescents. Published data on IMD are limited Greece many EU countries. In the present study, patients under 16 years of age with were retrospectively identified from files Hellenic National Meningitis Reference Laboratory (HNML) 2010–2020, their medical records tracked corresponding hospitals. Demographic, clinical,...
ABSTRACT Identification of clinical isolates Neisseria meningitidis that are resistant to rifampin is important avoid prophylaxis failure in contacts patients, but it hindered by the absence a breakpoint for resistance, despite many efforts toward standardization. We examined large number ( n = 392) meningococcal isolates, spanning 25 years (1984 2009), were collected 11 European countries, Argentina, and Central African Republic. The collection comprises all with MICs ≥0.25 mg/liter 161)...
Neisseria meningitidis is differentiated into 12 distinct serogroups, of which A, B, C, W-135, X, and Y are medically most important represent an health problem in different parts the world. The epidemiology N. unpredictable over time across geographic regions. Recent epidemiological surveillance has indicated increase serogroup invasive meningococcal disease some Europe as shown data for 2010 from various European countries previously published this journal.Citation1 Here, reported...
Invasive meningococcal disease (IMD) is an acute life-threatening infection caused by the gram-negative bacterium, Neisseria meningitidis. Globally, there are approximately half a million cases of IMD each year, with incidence varying across geographical regions. Vaccination has proven to be successful against IMD, as part controlling outbreaks, and when incorporated into national immunization programs. The South-Eastern Europe Meningococcal Advocacy Group (including representatives from...
Previous studies have shown that there is considerable variation in the methods and media used to determine susceptibility of Neisseria meningitidis antimicrobial agents different countries. In this study, national regional reference laboratories a standardized methodology MICs antibiotics management meningococcal infection. Fourteen participated determining penicillin G, rifampin, cefotaxime, ceftriaxone, ciprofloxacin, ofloxacin collection 17 meningococci, which 11 strains were previously...
Neisseria meningitidis is differentiated into 12 distinct serogroups, of which A, B, C, W, X, and Y are medically most important represent an health problem in different parts the world. The epidemiology N. unpredictable over time across geographic regions. Recent epidemiological surveillance has indicated increase serogroup invasive meningococcal disease some Europe as shown data for 2010 2011 from various European countries previously published this journal.Citation1,Citation2 Here, 33...