A5044485482- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- Hepatitis B Virus Studies
- Viral Infections and Vectors
- Biosensors and Analytical Detection
- Viral Infections and Outbreaks Research
- Malaria Research and Control
- Peripheral Artery Disease Management
- Vascular anomalies and interventions
- Vasculitis and related conditions
- Hepatitis C virus research
- Respiratory viral infections research
- Immune responses and vaccinations
- Central Venous Catheters and Hemodialysis
- Venous Thromboembolism Diagnosis and Management
- Abdominal vascular conditions and treatments
- Tribology and Lubrication Engineering
- Liver Disease Diagnosis and Treatment
- Diabetic Foot Ulcer Assessment and Management
- COVID-19 diagnosis using AI
Tunis University
2021-2025
Tunis El Manar University
2020-2025
Institut Pasteur de Tunis
2020-2025
University of Monastir
2021-2024
Institut Pasteur
2023
Institut Pasteur de Dakar
2022
Hôpital Charles-Nicolle
2017
Hospital Fatuma Bourguiba Monastir
2012
Documenting the circulation dynamics of SARS-CoV-2 variants in different regions world is crucial for monitoring virus transmission worldwide and contributing to global efforts towards combating pandemic. Tunisia has experienced several waves COVID-19 with a significant number infections deaths. The present study provides genetic information on lineages that circulated over 17 months. Lineages were assigned 1359 samples using whole-genome sequencing, partial S gene sequencing...
Introduction The Delta variant posed an increased risk to global public health and rapidly replaced the pre-existent variants worldwide. In this study, genetic diversity spatio-temporal dynamics of 662 SARS-CoV2 genomes obtained during wave across Tunisia were investigated. Methods Viral whole genome partial S-segment sequencing was performed using Illumina Sanger platforms, respectively lineage assignemnt assessed Pangolin version 1.2.4 scorpio 3.4.X. Phylogenetic phylogeographic analyses...
The emergence of SARS-CoV-2 variants has led to several cases among children. However, limited information is available from North African countries. This study describes the strains circulating in Tunisian pediatric population during successive waves. A total 447 complete sequences were obtained individuals aged 13 days 18 years, between March 2020 and September 2022: 369 generated this 78 ones, GISAID, previously patients. These compared with 354 274 ones adults a global dataset,...
Abstract Background Primary Immunodeficiency disorders (PID) can increase the risk of severe COVID-19 and prolonged infection. This study investigates duration SARS-CoV-2 excretion genetic evolution virus in pediatric PID patients as compared to immunocompetent (IC) patients. Materials methods A total 40 nasopharyngeal 24 stool samples were obtained from five ten IC children. RNA detection was performed using RT-qPCR, whole-genome sequencing conducted with NexSeq 1000 platform. Data analysis...
Since the World Health Organization declared Coronavirus Disease 2019 (COVID-19) pandemic as an international concern of public health emergency in early 2020, several strategies have been initiated many countries to prevent healthcare services breakdown and collapse structures. The most important strategy was increased testing, diagnosis, isolation, contact tracing identify, quarantine test close contacts. In this context, finding a rapid, reliable affordable tool for COVID-19 screening...
The mass vaccination campaign against SARS-CoV-2 was started in Tunisia on 13 March 2021 by using progressively seven different vaccines approved for emergency use. Herein, we aimed to evaluate the humoral and cellular immunity subjects aged 40 years over who received one of following two-dose regimen SARS-CoV-2, namely mRNA-1273 or Spikevax (Moderna), BNT162B2 Comirnaty (Pfizer-BioNTech), Gam-COVID-Vac Sputnik V (Gamaleya Research Institute), ChAdOx1-S Vaxzevria (AstraZeneca), BIBP...
Abstract Introduction RT-PCR testing on nasopharyngeal swabs is a key component in the COVID-19 fighting, provided to use sensitive and specific SARS-CoV2 genome targets. In this study, we aimed evaluate compare 4 widely used WHO approved protocols real clinical specimens, decrypt reasons of diverging results propose recommendations for choice Methods 260 samples were randomly selected among tested between Week-16, 2020 week-16 2021, Institut Pasteur de Tunis, Tunisia, one referent...
Reliable serological assays are needed to understand the real impact of COVID-19. In order compare efficiency different COVID-19 vaccines used in National Vaccination Program Tunisia, we have developed a quantitative in-house ELISA. The ELISA is based on ectodomain SARS-CoV-2 Spike Baculovirus recombinant protein. We panel 145 RT-PCR positive serum samples and 116 pre-pandemic as negative panel. validation was carried out by comparison four commercial techniques (Vidas IgG anti-RBD...
Background The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for serious infections in humans. Even the absence of symptoms, gastrointestinal (GI) signs were commonly reported adults and children. Thus, oral–fecal transmission was suspected as a possible route infection. objective this study to describe RNA shedding nasopharyngeal stool samples obtained from asymptomatic symptomatic children investigate virus viability. Methods This included 179 191 71 children,...
Recent efforts have reported numerous variants that influence severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral characteristics, including pathogenicity, transmission rate, and detectability by molecular tests. Whole-genome sequencing based on next-generation technologies is the method of choice to identify all variants; however, resources needed use these techniques for a representative number specimens remain limited in many low- middle-income countries. To decrease costs,...
(1) Background: This study aimed to compare the immunogenicity of mix-and-match CoronaVac/BNT162b2 vaccination homologous CoronaVac/CoronaVac regimen. (2) Methods: We conducted a simple-blinded randomized superiority trial measure SARS-CoV-2 neutralization antibodies and anti-spike receptor binding domain (RBD) IgG concentrations in blood samples participants who had received first dose CoronaVac vaccine followed by BNT162b2 or vaccine. The primary endpoint for was serum-neutralizing...
West Nile Virus (WNV) causes a serious public health concern in many countries around the world. detection pathological samples is key component of WNV infection diagnostic, classically performed by real-time PCR. In outbreak situation, rapid virus, peripheral laboratories or at point care, crucial to guide decision makers and for establishment adequate action plans prevent virus dissemination. Here, we evaluate Loop-mediated isothermal amplification (LAMP) tool detection. Amplifications...
Abstract Background In Tunisia a first SARS-CoV-2 confirmed case was reported in March 03, 2020. Since then, an increase of cases number observed from either imported or local cases. The aim this preliminary study to better understand the molecular epidemiology and genetic variability viruses circulating worldwide. Methods Whole genome sequencing performed using NGS approach on six SARS. CoV-2 highly positive samples detected during early phase outbreak. Results Full genomes sequences...
Hepatitis B virus (HBV) infection remains a serious public health concern worldwide despite the availability of an efficient vaccine and major improvements in antiviral treatments. The aim present study is to analyze mutational profile HBV whole genome ETV non-responder chronic patients, order investigate drug resistance, immune escape, liver disease progression Liver Cirrhosis (LC) or Hepatocellular Carcinoma (HCC). Blood samples were collected from five hepatitis patients. For each...
Full genomes sequences of six Tunisian SARS-CoV-2 strains were obtained from imported and locally transmission cases during the COVID-19 outbreak. Reported non-identical with 0.1% nucleotide divergence rate clustered into 6 different clades worldwide sequences. SNPs results favor distribution reported 3 major genotypes. These indicate multiple introductions virus in Tunisia add new genomic data on at international level.
Introduction: SARS-CoV2 serology testing is multipurpose provided to choose an efficient test. We evaluated and compared 4 different commercial tests, three of them had the Food Drug Administration (FDA) approval. Our goal was provide new data help guide interpretation choice serological tests. Methods: Four tests were evaluated: Cobas®Roche®(total anti-N antibodies), VIDAS®Biomerieux®(IgM IgG anti-RBD Mindray®(IgM antibodies) Access®Beckman Coulter®(IgG antibodies). Were tested: a positive...
Background: Allogeneic hematopoietic stem cell transplantation (ASCT) induces acquired immunodeficiency, potentially altering vaccine response. Herein, we aimed to explore the clinical tolerance and humoral cellular immune responses following anti-SARS-CoV-2 vaccination in ASCT recipients. Methods: A prospective, non-randomized, controlled study that involved 43 subjects 31 healthy controls. Humoral response was investigated using Elecsys® test anti-SARS-CoV-2. Cellular assessed QFN®...
Background: This study aimed to compare the immunogenicity of heterologous prime-boost CoronaVac/BNT162b2 vaccination homologous CoronaVac/CoronaVac regimen.Methods: We conducted a simple-blinded randomized superiority trial measure SARS-CoV-2 neutralization antibodies and anti-spike receptor binding domain (RBD) IgG concentrations in blood samples collected participants who had received first dose CoronaVac vaccine followed by BNT162b2 or vaccine. Participants were consenting adults with no...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for COVID-19 disease which known to have a broad clinical spectrum, from asymptomatic critical presentation leading death. Many researchers investigated the factors impacting course of disease. Our previous in silico study suggested possible protective effect Hepatitis B, Tetanus and Measles vaccines against COVID-19. In continuity, we conducted cross-sectional order confirm our assumptions regarding HBs-Ag...
Introduction: SARS-CoV-2 serology have several indications. Currently, as there are various types available, it is important to master their performance in order choose the best test for indication. We evaluated and compared four different commercial tests, three of them had Food Drug Administration Emergency Use Authorization (FDA-EUA). Our goal was provide new data help guide interpretation choice serological tests. Methods: Four tests were studied: Elecsys® Roche® on Cobas® (total...