A5042098318- Microbial bioremediation and biosurfactants
- Pesticide and Herbicide Environmental Studies
- Microbial Community Ecology and Physiology
- Insect behavior and control techniques
- Pharmaceutical and Antibiotic Environmental Impacts
- Insect and Pesticide Research
- Neurobiology and Insect Physiology Research
- Insect-Plant Interactions and Control
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Wastewater Treatment and Nitrogen Removal
- Insect Utilization and Effects
- Bacterial Genetics and Biotechnology
- Insect Pest Control Strategies
- Insect Resistance and Genetics
- Cancer Research and Treatments
- Insect symbiosis and bacterial influences
- Forest Insect Ecology and Management
- Enzyme-mediated dye degradation
- Renal and related cancers
- Biochemical and biochemical processes
- Enzyme Structure and Function
- Zebrafish Biomedical Research Applications
- Legume Nitrogen Fixing Symbiosis
- Entomopathogenic Microorganisms in Pest Control
Commonwealth Scientific and Industrial Research Organisation
2013-2025
Macquarie University
2020-2024
Heidelberg University
2018-2023
University Hospital Heidelberg
2018-2023
Jawaharlal Nehru Medical College
2023
CSIRO Land and Water
2014-2022
Datta Meghe Institute of Medical Sciences
2019-2021
Maharaja Krishna Chandra Gajapati Medical College and Hospital
2021
Jawaharlal Nehru Medical College
2021
ACT Government
2007-2020
Abstract Here, we compare the evolutionary routes by which bacteria and insects have evolved enzymatic processes for degradation of four classes synthetic chemical insecticide. For insects, selective advantage such degradative activities is survival on exposure to insecticide, whereas simply a matter access additional sources nutrients. Nevertheless, highly efficient enzymes from wide variety enzyme families, relied upon generalist esterase‐, cytochrome P450‐ glutathione‐ S...
The metabolism of volatile signal molecules by odorant degrading enzymes (ODEs) is crucial to the ongoing sensitivity and specificity chemoreception in various insects, a few specific esterases, cytochrome P450s, glutathione S-transferases (GSTs) UDP-glycosyltransferases (UGTs) have previously been implicated this process. Significant progress has made characterizing ODEs Lepidoptera but very little known about them Diptera, including Drosophila melanogaster, major insect model. We therefore...
Insect carboxylesterases from the α Esterase gene cluster, such as αE7 (also known E3) Australian sheep blowfly Lucilia cuprina ( Lc αE7), play an important physiological role in lipid metabolism and are implicated detoxification of organophosphate (OP) insecticides. Despite importance OPs to agriculture spread insect-borne diseases, molecular basis for ability α-carboxylesterases confer OP resistance insects is poorly understood. In this work, we used laboratory evolution increase thermal...
Biting midges (Culicoides spp.) are important vectors of several insect borne arboviruses but underrepresented in terms availability high-resolution genomic resources. We assembled and annotated complete mitochondrial genomes for two Culicoides species, namely C. brevitarsis imicola which proven Bluetongue Virus (BTV). used both long read short sequencing technologies to assemble the circular genomes. The genome sizes 17,100 bp 17,031 bp, respectively, all comprising 37 genes, including 13...
Per- and poly-fluoroalkyl substances (PFAS) pose a threat to organisms ecosystems due their persistent nature. Ecotoxicology endpoints used in regulatory guidelines may not reflect multiple, low-level but stressors. This study examines the biological effects of PFAS on Eastern short-necked turtles Queensland, Australia. In this study, blood samples were collected analysed for PFAS, hormone levels, functional omics endpoints. High levels found at impacted site, with PFOS being dominant...
2,4-Dinitroanisole (DNAN) is an insensitive munition ingredient used in explosive formulations as a replacement for 2,4,6-trinitrotoluene (TNT). Little known about the environmental behavior of DNAN. There are reports microbial transformation to dead-end products, but no bacteria with complete biodegradation capability have been reported. Nocardioides sp. strain JS1661 was isolated from activated sludge based on its ability grow DNAN sole source carbon and energy. Enzyme assays indicated...
Reception of odorant molecules within insect olfactory organs involves several sequential steps, including their transport through the sensillar lymph, interaction with respective sensory receptors, and subsequent inactivation. Odorant-Degrading Enzymes (ODEs) putatively play a role in signal dynamics by rapid degradation odorants vicinity but this hypothesis is mainly supported vitro results. We have recently shown that an extracellular carboxylesterase, esterase-6 (EST-6), involved...
Pest management programs can operate more effectively when movement patterns of target species are known. As individual insects difficult to track, genomic data instead be used infer based on pest population structure and connectivity. These also provide critical information about cryptic taxa relevant management. Here we present the first investigation Aedes vigilax, Australian saltmarsh mosquito, a major arbovirus vector across Australasia. We ddRAD pool-seq approach draft genome assembly...
Biodegradation of p-nitrophenol (PNP), a priority pollutant, was studied as model system for bioremediation sites contaminated with nitroaromatic/organic compounds. Bioremediation PNP-containing soil first carried out in pots using immobilized and free cells Arthrobacter protophormiae RKJ100 order to ascertain the role suitable carrier material. Results showed that stability introduced strain enhanced upon immobilization rate PNP depletion decreased increasing depth soil. Small-scale field...
The introduction of culture-independent molecular screening techniques, especially based on 16S rRNA gene sequences, has allowed microbiologists to examine a facet microbial diversity not necessarily reflected by the results culturing studies. bacterial community structure was studied for pesticide-contaminated site that subsequently remediated using an efficient degradative strain Arthrobacter protophormiae RKJ100. efficiency bioremediation process assessed monitoring depletion pollutant,...
Mycobacterium brisbanense strain JK1, a bacterium capable of degrading the herbicide diuron, was isolated from herbicide-exposed soil. A gene/enzyme system with diuron hydrolase activity this and named PUH (phenylurea hydrolase) B (puhB/PuhB) because its close similarity to previously characterized (puhA/PuhA). Both PUHs were heterologously expressed, purified characterized. The found oligomerize as hexamers in solution, each monomer containing mononuclear Zn2+ active site. Sequence analysis...
Microbial degradation studies have pointed toward the occurrence of two distinct PNP catabolic pathways in Gram positive and negative bacteria. The former involves 4-nitrocatechol (4-NC), 1,2,4-benzenetriol (BT), maleylacetate (MA) as major intermediates, whereas later proceeds via formation 1,4-benzoquinone (BQ) hydroquinone (HQ). In present study we identified a organism viz. Burkholderia sp. strain SJ98 that degrades 4NC, BT, MA. A 6.89 Kb genomic DNA fragment encompasses seven putatively...