A5035649809- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
- Insect Resistance and Genetics
- Insect-Plant Interactions and Control
- Insect and Pesticide Research
- Insect Pest Control Strategies
- Insect and Arachnid Ecology and Behavior
- Malaria Research and Control
- Studies on Chitinases and Chitosanases
- Dengue and Mosquito Control Research
- Parasitic Diseases Research and Treatment
- Entomopathogenic Microorganisms in Pest Control
- Insect behavior and control techniques
- CO2 Reduction Techniques and Catalysts
- Botany, Ecology, and Taxonomy Studies
- Genetic diversity and population structure
- Viral Infections and Vectors
- Insect Utilization and Effects
- Plant and animal studies
- Parasite Biology and Host Interactions
- Vector-borne infectious diseases
- Rangeland and Wildlife Management
- Cocoa and Sweet Potato Agronomy
- Study of Mite Species
The University of Melbourne
2016-2025
AgriBio
2020
Dengue has enormous health impacts globally. A novel approach to decrease dengue incidence involves the introduction of Wolbachia endosymbionts that block virus transmission into populations primary vector mosquito, Aedes aegypti. The wMel strain previously been trialed in open releases Ae. aegypti; however, wAlbB shown maintain higher density than at high larval rearing temperatures. Releases aegypti mosquitoes carrying were carried out 6 diverse sites greater Kuala Lumpur, Malaysia, with...
Aedes aegypti mosquitoes infected with Wolbachia bacteria are currently being released for arbovirus suppression around the world. Their potential to invade populations and persist will depend on interactions environmental conditions, particularly as larvae often exposed fluctuating extreme temperatures in field. We reared Ae. different types of (wMel, wAlbB wMelPop-CLA) under diurnal cyclical temperatures. Rearing wMel wMelPop-CLA-infected at 26-37°C reduced expression cytoplasmic...
Mutations in the voltage-sensitive sodium channel gene (Vssc) have been identified Aedes aegypti and some associated with pyrethroid insecticide resistance. Whether these mutations cause resistance, alone or combination other alleles, remains unclear, but must be understood if are to become markers for resistance monitoring. We describe High Resolution Melt (HRM) genotyping assays assessing found Ae. Indonesia (F1565C, V1023G, S996P) use them test associations mosquitoes from Yogyakarta, a...
Abstract Modified Aedes aegypti mosquitoes reared in laboratories are being released around the world to control wild mosquito populations and diseases they transmit. Several efforts have failed due poor competitiveness of mosquitoes. We hypothesized that colonized could suffer from inbreeding depression adapt laboratory conditions, reducing their performance field. established replicate Ae. collected Queensland, Australia, maintained them for twelve generations at different census sizes....
Aedes aegypti and Ae. albopictus are important vectors of infectious diseases, especially those caused by arboviruses such as dengue, chikungunya Zika. is very well adapted to urban environments, whereas inhabits more rural settings. Pyrethroid resistance widespread in these vectors, but limited data exist from the Southwest Pacific Region, Melanesia. While vector ecology documented Australia, where incursion pyrethroid have so far been prevented, almost nothing known about populations...
Biological invasions are increasing globally in number and extent despite efforts to restrict their spread. Knowledge of incursion pathways is necessary prevent new design effective biosecurity protocols at source recipient locations. This study uses genome-wide single nucleotide polymorphisms (SNPs) determine the origin 115 incursive
Nations throughout the Indo-Pacific region use pyrethroid insecticides to control Aedes aegypti, mosquito vector of dengue, often without knowledge resistance status pest or origin resistance. Two mutations (V1016G + F1534C) in sodium channel gene (Vssc) Ae. aegypti modify ion function and cause target-site insecticides, with a third mutation (S989P) having potential additive effect. Of 27 possible genotypes involving these mutations, some allelic combinations are never seen whereas others...
Pest management programs can operate more effectively when movement patterns of target species are known. As individual insects difficult to track, genomic data instead be used infer based on pest population structure and connectivity. These also provide critical information about cryptic taxa relevant management. Here we present the first investigation Aedes vigilax, Australian saltmarsh mosquito, a major arbovirus vector across Australasia. We ddRAD pool-seq approach draft genome assembly...
Abstract Dengue is the most prevalent global arboviral disease that affects over 300 million people every year. Brazil has highest number of dengue cases in world, with severe epidemics city Rio de Janeiro (Rio). The effective control critically dependent on knowledge population genetic structuring primary vector, mosquito Aedes aegypti . We analyzed mitochondrial and nuclear genomewide single nucleotide polymorphism markers generated via Restriction‐site Associated DNA sequencing, as well...
Dengue fever, the most prevalent global arboviral disease, represents an important public health problem in Indonesia. Control of dengue relies on control its main vector, mosquito Aedes aegypti, yet nothing is known about population history and genetic structure this insect Our aim was to assess spatio-temporal Ae. aegypti Yogyakarta, a densely populated region Java with common outbreaks. We used multiple marker systems (microsatellites, nuclear mitochondrial genome-wide single nucleotide...
Aedes aegypti mosquitoes experimentally infected with Wolbachia are being utilized in programs to control the spread of arboviruses such as dengue, chikungunya and Zika. Wolbachia-infected can be released into field either reduce population sizes through incompatible matings or transform populations that refractory virus transmission. For these strategies succeed, from laboratory must competitive native mosquitoes. However, maintaining result inbreeding, genetic drift adaptation which their...
Aedes mosquitoes harboring intracellular Wolbachia bacteria are being released in arbovirus and mosquito control programs. With releases taking place around the world, understanding contribution of host variation to phenotype is crucial. We generated a transinfection (wAlbBQ) aegypti performed backcrossing introduce infection into Australian or Malaysian nuclear backgrounds. Whole genome sequencing shows that wAlbBQ nearly identical reference wAlbB genome, suggesting few changes since was...
There is growing interest in insecticide resistance the mosquito, Aedes albopictus (Skuse), as its potential for spreading diseases increasing urbanization and control efforts intensify. Here we review presence diversity of mutations voltage-sensitive sodium channel (Vssc) gene associated with pyrethroid report on additional surveys these new populations an analysis their spread. The known has increased recent years including identification 26 non-synonymous mutations, although phenotypic...
Wolbachia is the most widespread endosymbiotic bacterium of insects and other arthropods that can rapidly invade host populations. Deliberate releases into natural populations dengue fever mosquito, Aedes aegypti, are used as a novel biocontrol strategy for suppression. Invasion through population relies on factors such high fidelity endosymbiont transmission limited immigration uninfected individuals, but these be difficult to measure. One way acquiring relevant information consider...
Although pesticide resistance is common in insect vectors of human diseases, the evolution might be delayed if management practices are adopted that limit selection alleles. Outbreaks dengue fever have occurred Queensland, Australia, since late 1800s, leading to ongoing attempts control mosquito vector, Aedes aegypti (L.). Since 1990s, pyrethroid insecticides been used for this purpose, but applied a strategic manner with variety delivery methods including indoor residual spraying, lethal...
In recent decades, the occurrence and distribution of arboviral diseases transmitted by Aedes aegypti mosquitoes has increased. a new control strategy, populations infected with Wolbachia are being released to replace existing suppress disease transmission. The success this strategy can be affected high temperature exposure, but impact low temperatures on Wolbachia-infected Ae. is unclear, even though restrict abundance species. study, we considered cycles relevant spring season that close...
The equine South African pointy vector mosquito, Aedes caballus , poses a significant threat to human health due its capacity for transmitting arboviruses. Despite favorable climate existence in southeast Iran, previous records of this species the area have indicated very low abundance. This comprehensive field and laboratory study aimed assess current adult population status region, utilizing combination ecological, morphological molecular techniques. Four distinct types traps were...
Abstract Wolbachia-infected Aedes aegypti (L.) mosquitoes for control of dengue transmission are being released experimentally in tropical regions Australia, south-east Asia, and South America. To become established, the Wolbachia Hertig (Rickettsiales: Rickettsiaceae) strains used must induce expression cytoplasmic incompatibility (CI) matings between infected males uninfected females so that have a reproductive advantage, which will drive infection through field populations. is...
Abstract Aedes aegypti (yellow fever mosquito) and Ae. albopictus (Asian tiger are globally invasive pests that confer the world’s dengue burden. Insecticide-based management has led to evolution of insecticide resistance in both species, though genetic architecture geographical spread remains incompletely understood. This study investigates partial selective sweeps at genes on two chromosomes characterises their across populations. Sweeps voltage-sensitive sodium channel ( VSSC ) gene...
Insecticide resistance (IR) monitoring is essential for evidence-based control of mosquito-borne diseases. While widespread pyrethroid in Anopheles and Aedes species has been described many countries, data Papua New Guinea (PNG) are limited. Available indicate that the local populations PNG remain pyrethroid-susceptible, making regular IR even more important. In addition, aegypti PNG. Here, generated from across between 2017 2022 presented.
Wolbachia , a gram-negative endosymbiotic bacterium widespread in arthropods, is well-known for changing the reproduction of its host ways that increase rate spread, but there are also costs to hosts can reduce this. Here we investigated novel reproductive alteration w AlbB on Aedes aegypti using studies mosquito life history traits, ovarian dissection, as well gene expression assays. We found an extended period larval stage egg (as previously shown) proportion -infected females become...
Host seeking is an essential process in mosquito reproduction. Field releases of modified mosquitoes for population replacement rely on successful host by female mosquitoes, but host-seeking ability rarely tested a realistic context. We the Aedes aegypti using semi-field system. Females with different Wolbachia infection types ( w Mel-, AlbB-infected, and uninfected) or from origins (laboratory field) were released at one end cage recaptured as they landed human experimenters 15 m away....
Abstract Aedes notoscriptus (Skuse) is a container-inhabiting mosquito endemic to Australia that vectors arboviruses and suspected transmit Mycobacterium ulcerans, the cause of Buruli ulcer. We evaluated effectiveness In2Care station, which suppresses populations via entomopathogenic fungus, Beauveria bassiana, insect growth regulator pyriproxyfen, latter autodisseminated among larval habitats by contaminated mosquitoes. A field trial was conducted using 110 stations in 50,000 m2 area...