A5082916004- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Insect symbiosis and bacterial influences
- Dengue and Mosquito Control Research
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- COVID-19 diagnosis using AI
- Insect and Pesticide Research
- vaccines and immunoinformatics approaches
- Viral Infections and Vectors
- Sepsis Diagnosis and Treatment
- Malaria Research and Control
- SARS-CoV-2 detection and testing
- Pneumonia and Respiratory Infections
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Neonatal and Maternal Infections
- Public Health and Nutrition
- Nosocomial Infections in ICU
- Long-Term Effects of COVID-19
- HIV/AIDS Research and Interventions
- Immunotherapy and Immune Responses
- Pericarditis and Cardiac Tamponade
- Bacterial Infections and Vaccines
- Dental Anxiety and Anesthesia Techniques
Universitas Gadjah Mada
2016-2025
Rumah Sakit Umum Pusat Dr. Sardjito
2016-2024
ADA University
2023
Ito Hospital
2021
Chiba University
2005-2012
Institut thématique Génétique, génomique et bioinformatique
2005
Aedes aegypti mosquitoes infected with the wMel strain of Wolbachia pipientis are less susceptible than wild-type A. to dengue virus infection.We conducted a cluster-randomized trial involving releases wMel-infected for control in Yogyakarta, Indonesia. We randomly assigned 12 geographic clusters receive deployments (intervention clusters) and no (control clusters). All practiced local mosquito-control measures as usual. A test-negative design was used assess efficacy intervention. Patients...
<ns4:p><ns4:bold>Background: </ns4:bold><ns4:italic>Ae. aegypti</ns4:italic> mosquitoes stably transfected with the intracellular bacterium <ns4:italic>Wolbachia</ns4:italic> <ns4:italic>pipientis</ns4:italic> (<ns4:italic>w</ns4:italic>Mel strain) have been deployed for biocontrol of dengue and related arboviral diseases in multiple countries. Field releases northern Australia previously demonstrated near elimination local transmission from <ns4:italic>Wolbachia</ns4:italic>-treated...
The successful establishment of the wMel strain Wolbachia for control arbovirus transmission by Aedes aegypti has been proposed and is being implemented in a number countries. Here we describe four sites Yogyakarta, Indonesia. We demonstrate that can be successfully introgressed after transient releases wMel-infected eggs or adult mosquitoes. approach acceptable to communities maintains itself mosquito population once deployed. Finally, our data show spreading rates Indonesian setting are...
Dengue and other arboviruses transmitted by Aedes aegypti mosquitoes, including Zika chikungunya, present an increasing public health challenge in tropical regions. Current vector control strategies have failed to curb disease transmission, but continue be employed despite the absence of robust evidence for their effectiveness or optimal implementation. The World Mosquito Program has developed a novel approach arbovirus using Ae. stably transfected with Wolbachia bacterium, significantly...
The AWED (Applying Wolbachia to Eliminate Dengue) trial is a parallel, two-arm, non-blinded cluster randomised controlled that under way in Yogyakarta, Indonesia, with the aim of measuring efficacy Wolbachia-infected Aedes aegypti deployments reducing dengue incidence an endemic setting. Enrolment began January 2018 and ongoing. original study protocol was published April 2018. Here, we describe amendments have been made since commencement trial.The key are (1) revised duration planned end...
The Bcl6 proto-oncogene, which encodes a transcriptional repressor, is ubiquitously expressed and predominantly in germinal center (GC) B cells. Although the promoter region of human gene has been reported, enhancer molecules for its high expression GC cells were largely unknown. Here we show that start sites murine different from reported one. DNA sequence around new highly conserved between mice humans no canonical TATA or CCAAT box. Two AP-1-binding elements major GC-derived lymphoma...
Background This study describes the seroconversion and serodynamics of IgG antibodies against RBD SARS-CoV-2 in general population Sleman District, Yogyakarta Special Province. We aim to identify possible factors that correlate with SARS-CoV-2. Methods performed a longitudinal at Health Demographic Surveillance System (HDSS) Sleman, Yogyakarta, Indonesia. Study subjects were recruited between April December 2021 using convenience sampling followed up 2 times, i.e. 4–5 8–9 weeks. The...
Background Circulating markers of immune and endothelial activation risk stratify infection syndromes agnostic to disease aetiology. However, their utility in children presenting from the community remains unclear. Methods This study recruited aged 1-59 months with community-acquired acute febrile illnesses seven hospitals Bangladesh, Cambodia, Indonesia, Laos, Viet Nam. Clinical parameters biomarker concentrations were measured at presentation. The outcome measure was death or receipt vital...
The introgression of w Mel Wolbachia into Aedes aegypti populations is being used for the biocontrol arboviruses such as dengue and chikungunya in 14 countries to date. A infection Ae. both reduces transmission viruses by mosquito causes a reproductive manipulation that aids naive populations. However, critical concern whether could evolve over time, potentially diminishing these desired phenotypes. Here, we investigated stability genome released Colombia, Indonesia, Vietnam. We sequenced at...
Marsha S. Santoso, M.D, M.I.P.H., Eggi Arguni, Ph.D., Bunga Rana, M.Sc., Mercy E. Adiniko, Dionisius Denis, Endah Supriyati, Citra Indriani, M.P.H., Leily Trianty, Riris Andono Ahmad, Rintis Noviyanti, and R. Tedjo Sasmono, Ph.D.. Ann Lab Med -0001;0:. https://doi.org/10.3343/alm.2024.0558
Expression of B lymphocyte-induced maturation protein 1 (Blimp-1) transcription factor is essential for promoting cell differentiation into plasma cells. However, a critical Blimp-1 expression in activated cells unclear. When splenic were stimulated with CD40 ligand (CD40L) and IL-4, terminal was induced the from c-fos transgenic (H2-c-fos) mice but barely those control littermates c-fos-deficient mice. AP-1 family mRNAs transiently cells, overexpression c-Fos sufficient amount H2-c-fos...
Dengue fever, the most prevalent global arboviral disease, represents an important public health problem in Indonesia. Control of dengue relies on control its main vector, mosquito Aedes aegypti, yet nothing is known about population history and genetic structure this insect Our aim was to assess spatio-temporal Ae. aegypti Yogyakarta, a densely populated region Java with common outbreaks. We used multiple marker systems (microsatellites, nuclear mitochondrial genome-wide single nucleotide...
Background In 2017, a diphtheria outbreak occurred in several provinces Indonesia. The aim of this study was to identify predictors mortality outcome pediatric patients with clinical diphtheria. Methods A retrospective cohort conducted using patient medical records at five referral hospitals the Province Jakarta and one Tangerang District, Banten during January 2017 31 August 2018. All children age group 1–18 years old discharged diagnosis formed group. anonymized data were evaluated for...
Bcl6, a sequence-specific transcriptional repressor, is important for generation and maintenance of memory CD8(+) T cells. Although cells are generated from effector cells, role Bcl6 in largely unknown. We show here that expression was transiently induced activated continuously up-regulated The amount granzyme B mRNA among molecules produced by inversely correlated with the Overexpression resulted lower killing activity at their phase, supporting reduction Bcl6. identified putative...
Wolbachia is the most widespread endosymbiotic bacterium of insects and other arthropods that can rapidly invade host populations. Deliberate releases into natural populations dengue fever mosquito, Aedes aegypti, are used as a novel biocontrol strategy for suppression. Invasion through population relies on factors such high fidelity endosymbiont transmission limited immigration uninfected individuals, but these be difficult to measure. One way acquiring relevant information consider...
In southern Papua, Indonesia, malaria is highly prevalent in young children and a significant cause of morbidity early mortality. The association between delayed mortality unknown.Routinely-collected hospital surveillance data from were used to assess the risk recurrent within 12 months an initial presentation with all younger than 5 years old attending local hospital. Analysis was primarily by Kaplan Meier Cox regression methods.In total 15,716 presenting April 2004 December 2013 included...
Background: Severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) Delta variant (B.1.617.2) has been responsible for the current increase in disease 2019 (COVID-19) infectivity rate worldwide. We compared impact of and non-Delta on COVID-19 outcomes patients from Yogyakarta Central Java provinces, Indonesia. Methods: In this cross-sectional study, we ascertained 161 patients, 69 with 92 variant. The Illumina MiSeq next-generation sequencer was used to perform whole-genome sequences...
Dengue is endemic in Indonesia. Here, we describe the epidemiology of dengue city Yogyakarta, Central Java, as a prelude to implementation cluster-randomized trial Wolbachia for biocontrol arboviral transmission. Surveillance records from 2006 2016 demonstrate seasonal oscillations incidence with varying magnitude. Two lines evidence high force infection; hospitalized case burden patients diagnosed hemorrhagic fever or shock syndrome over last decade consisted predominantly...
Indonesia is one of the countries where dengue infection prevalent. In this study we measure prevalence and distribution virus (DENV) DENV-infected Aedes aegypti in Yogyakarta City, Indonesia, during wet season when high transmission period occurred, as baseline data before implementation a Wolbachia-infected trial for control. We applied One-Step Multiplex Real Time PCR (RT-PCR) type-specific-detection viruses field-caught adult mosquitoes. prospective field conducted from December 2015 to...
Background Recently, SARS-CoV-2 virus with the D614G mutation has become a public concern due to rapid dissemination of this variant across many countries. Our study aims were (1) report full-length genome sequences collected from four COVID-19 patients in Special Region Yogyakarta and Central Java provinces, Indonesia; (2) compare clade distribution Indonesia ( n = 60) March September 2020 (3) perform phylogenetic analysis complete genomes different countries, including Indonesia. Methods...
Abstract Background Transmission within families and multiple spike protein mutations have been associated with the rapid transmission of SARS-CoV-2. We aimed to: (1) describe full genome characterization SARS-CoV-2 correlate sequences epidemiological data family clusters, (2) conduct phylogenetic analysis all samples from Yogyakarta Central Java, Indonesia other countries. Methods The study involved 17 patients COVID-19, including two clusters. determined full-genome using Illumina MiSeq...
The Applying Wolbachia to Eliminate Dengue (AWED) trial was a parallel cluster randomised that demonstrated ( w Mel) introgression into Ae . aegypti populations reduced dengue incidence. In this predefined substudy, we compared between treatment arms, the relative abundance of and albopictus before, during after Mel-introgression. Between March 2015 2020, 60,084 BG trap collections yielded 478,254 17,623 arms there no measurable difference in before or Mel-deployments, with count ratio 0.96...
Growing evidence shows that viral co-infection is found repeatedly in patients with Coronavirus Disease-2019 (COVID-19). This the first report of SARS-CoV-2 respiratory pathogens Indonesia.Over a one month period April to May 2020, positive nasopharyngeal swabs our COVID-19 referral laboratory Yogyakarta, Indonesia, were tested for by real-time, reverse transcription polymerase chain reaction (RT-PCR). Proportion reported percentage.Fifty-nine samples other among total 125 samples. Influenza...