A5031378200- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
- Molecular Biology Techniques and Applications
- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Insect-Plant Interactions and Control
- Genomics and Phylogenetic Studies
- Insect behavior and control techniques
- Insect and Pesticide Research
- Dengue and Mosquito Control Research
- Infections and bacterial resistance
- Plant and Fungal Interactions Research
- Plant Pathogenic Bacteria Studies
- Viral Infections and Vectors
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- COVID-19 epidemiological studies
- Invertebrate Immune Response Mechanisms
- Plant-Microbe Interactions and Immunity
- RNA and protein synthesis mechanisms
- Industrial Vision Systems and Defect Detection
- Insect and Arachnid Ecology and Behavior
- Forest Insect Ecology and Management
- Entomopathogenic Microorganisms in Pest Control
- Coleoptera Taxonomy and Distribution
- Plant Virus Research Studies
QIMR Berghofer Medical Research Institute
2017-2024
The University of Queensland
2019-2024
The University of Melbourne
2014-2018
Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of developing tools to fight them has been slowed by lack a high-quality genome assembly. Here we combine diverse technologies produce markedly improved, fully re-annotated AaegL5 assembly, demonstrate how it accelerates mosquito science. We anchored physical cytogenetic maps, doubled number...
Genetic markers are widely used to understand the biology and population dynamics of disease vectors, but often limited in resolution they provide. In particular, delineation structure, fine scale movement patterns relatedness obscured unless numerous available. To address this issue major arbovirus vector, yellow fever mosquito (Aedes aegypti), we double digest Restriction-site Associated DNA (ddRAD) sequencing for discovery genome-wide single nucleotide polymorphisms (SNPs). We aimed...
Medically important arboviruses such as dengue, Zika, and chikungunya viruses are primarily transmitted by the globally distributed mosquito Aedes aegypti. Increasing evidence suggests that transmission can be influenced viromes. Herein RNA-Seq was used to characterize RNA metaviromes of wild-caught Ae. aegypti from Bangkok (Thailand) Cairns (Australia). The two populations showed a high degree similarity in their BLAST searches assembled contigs suggest up 27 insect-specific may infect...
The drivers and patterns of zoonotic virus emergence in the human population are poorly understood. mosquito Aedes aegypti is a major arbovirus vector native to Africa that invaded most world's tropical belt over past four centuries, after evolution "domestic" form specialized biting humans breeding water storage containers. Here, we show specialization subsequent spread A. out were accompanied by an increase its intrinsic ability acquire transmit emerging pathogen Zika virus. Thus, recent...
Mechanisms and evolutionary dynamics of sex-determination systems are particular interest in insect vectors human pathogens like mosquitoes because novel control strategies aim to convert pathogen-transmitting females into nonbiting males, or rely on accurate sexing for the release sterile males. In Aedes aegypti, main vector dengue Zika viruses, sex determination is governed by a dominant male-determining locus, previously thought reside within small, nonrecombining, sex-determining region...
Abstract Dengue is the most prevalent global arboviral disease that affects over 300 million people every year. Brazil has highest number of dengue cases in world, with severe epidemics city Rio de Janeiro (Rio). The effective control critically dependent on knowledge population genetic structuring primary vector, mosquito Aedes aegypti . We analyzed mitochondrial and nuclear genomewide single nucleotide polymorphism markers generated via Restriction‐site Associated DNA sequencing, as well...
Dengue fever, the most prevalent global arboviral disease, represents an important public health problem in Indonesia. Control of dengue relies on control its main vector, mosquito Aedes aegypti, yet nothing is known about population history and genetic structure this insect Our aim was to assess spatio-temporal Ae. aegypti Yogyakarta, a densely populated region Java with common outbreaks. We used multiple marker systems (microsatellites, nuclear mitochondrial genome-wide single nucleotide...
Background The mosquito Aedes aegypti (L.) is a major vector of viral diseases like dengue fever, Zika and chikungunya. exhibits high morphological behavioral variation, some which thought to be epidemiological significance. Globally distributed domestic Ae. have often been grouped into (i) the very pale variety queenslandensis (ii) type form. Because two color forms co-occur across most their range, there interest in understanding how freely they interbreed. This knowledge particularly...
Hundreds of millions people get a mosquito-borne disease every year and nearly one million die. Transmission these infections is primarily tackled through the control mosquito vectors. The accurate quantification dispersal critical for design optimization vector programs, yet measurement using traditional mark-release-recapture (MRR) methods logistically challenging often unrepresentative an insect's true behavior. Using Aedes aegypti (a major arboviral vector) as model two study sites in...
Female Aedes aegypti mosquitoes infect hundreds of millions people each year with dangerous viral pathogens including dengue, yellow fever, Zika, and chikungunya. Progress in understanding the biology this insect, developing tools to fight it, has been slowed by lack a high-quality genome assembly. Here we combine diverse technologies produce AaegL5, dramatically improved annotated assembly, demonstrate how it accelerates mosquito science control. We anchored physical cytogenetic maps,...
The coconut rhinoceros beetle (CRB, Oryctes rhinoceros) is a severe and invasive pest of other palms throughout Asia the Pacific. biocontrol agent, nudivirus (OrNV), has successfully suppressed O. populations for decades but new CRB invasions started appearing after 2007. A single-SNP variant within mitochondrial cox1 gene used to distinguish recently-invading CRB-G lineage from haplotypes, lack mitogenome sequence this species hinders further development molecular toolset biosecurity...
Abstract Background An optimal starting point for relating genome function to organismal biology is a high-quality nuclear assembly, and long-read sequencing revolutionizing the production of this genomic resource in insects. Despite this, assemblies have been under-represented agricultural insect pests, particularly from order Coleoptera. Here we present de novo assembly structural annotation coconut rhinoceros beetle, Oryctes (Coleoptera: Scarabaeidae), based on Oxford Nanopore...
Abstract Over the last few years, various types of NGS data have been accumulating for coconut rhinoceros beetle (CRB, Oryctes ), reflecting growing interest in curtailing this invasive pest palm trees. Whilst reference-free analyses RNA-seq and RAD-seq datasets done different CRB collections, recent availability CRB’s genome assembly provides an opportunity to collate diverse create a reference-based population dataset. Here, I release such dataset containing 6,725,935 SNPs genotypes called...
Abstract The endosymbiotic bacterium Wolbachia suppresses the capacity for arboviral transmission in mosquito Aedes aegypti , and can spread through wild populations following local introductions. Recent introductions Cairns, Australia have demonstrated slower than expected spread, that could be due to: i) barriers to Ae. dispersal; ii) leptokurtically distributed dispersal distances; iii) intergenerational loss of . We investigated these three potential causes using genome-wide...
Abstract Mechanisms and evolutionary dynamics of sex-determination systems are particular interest in insect vectors human pathogens like mosquitoes because novel control strategies aim to convert pathogen-transmitting females into non-biting males, or rely on accurate sexing for the release sterile males. In Aedes aegypti, main vector dengue Zika viruses, sex determination is governed by a dominant male-determining locus, previously thought reside within small, non-recombining,...
Although homomorphic sex chromosomes can have non-recombining regions with elevated sequence divergence between its complements, such signals be difficult to detect bioinformatically. If found in genomes of e.g. insect pests, these sequences could targeted by the engineered genetic sexing and control systems. Here, we report an approach that leverage long-read nanopore sequencing a single XY male identify divergent chromosomes. Long-read data are used for de novo genome assembly is...
Abstract Background The mosquito Aedes aegypti (L.) is a major vector of viral diseases like dengue fever, Zika and chikungunya. exhibits high morphological behavioral variation, some which thought to be epidemiological significance. Globally distributed domestic Ae. have been traditionally grouped into (i) the very pale variety queenslandensis (ii) type form. Because two color forms co-occur across most their range, there interest in understanding how freely they interbreed. This knowledge...
ABSTRACT We report the complete circular genome assembly of Elizabethkingia anophelis (Flavobacteriales) generated with ONT and Illumina sequences from a laboratory-reared Aedes aegypti mosquito. This sequence does not belong to lineage known isolates Anopheles mosquitoes, indicating that E. is genomically diverse across mosquito disease vectors.
Abstract Background Hundreds of millions people get a mosquito-borne disease every year, which nearly one million die. Mosquito-borne diseases are primarily controlled and mitigated through the control mosquito vectors. Accurately quantified dispersal in given landscape is critical for design optimization programs, yet field experiments that measure mosquitoes recaptured at certain distances from release point (mark-release-recapture MRR studies) challenging such small insects often...