A5053451615- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- CRISPR and Genetic Engineering
- Dengue and Mosquito Control Research
- COVID-19 epidemiological studies
- Studies on Chitinases and Chitosanases
- Genetic diversity and population structure
- Chromosomal and Genetic Variations
- Evolution and Genetic Dynamics
- Insect Resistance and Genetics
- Genetic and phenotypic traits in livestock
- Viral Infections and Vectors
Institut Pasteur
2020-2024
Centre National de la Recherche Scientifique
2020-2024
Université Paris Cité
2022-2024
Department of Genomes & Genetics
2021
Endogenous viral elements (EVEs) are sequences integrated in host genomes. A large number of non-retroviral EVEs was recently detected Aedes mosquito genomes, leading to the hypothesis that may control exogenous infections by closely related viruses. Here, we experimentally investigated role an EVE naturally found aegypti populations and derived from widespread insect-specific virus, cell-fusing agent virus (CFAV). Using CRISPR-Cas9 genome editing, created Ae. line lacking CFAV EVE. Absence...
The drivers and patterns of zoonotic virus emergence in the human population are poorly understood. mosquito Aedes aegypti is a major arbovirus vector native to Africa that invaded most world's tropical belt over past four centuries, after evolution "domestic" form specialized biting humans breeding water storage containers. Here, we show specialization subsequent spread A. out were accompanied by an increase its intrinsic ability acquire transmit emerging pathogen Zika virus. Thus, recent...
Arthropod-borne viruses (arboviruses) transmitted by Aedes aegypti mosquitoes are an increasing threat to global health. The small interfering RNA (siRNA) pathway is considered the main antiviral immune of insects, but its effective impact on arbovirus transmission surprisingly poorly understood. Here, we use CRISPR-Cas9-mediated gene editing in vivo mutate Dicer2, a encoding sensor and key component siRNA pathway. loss Dicer2 enhances early viral replication systemic dissemination four...
The mosquito Aedes aegypti is the primary vector for dengue virus (DENV), which infects millions of people annually. Variability in DENV susceptibility among wild Ae. populations governed by genetic factors, but specific causal variants are unknown. Here, we identify a cytochrome P450-encoding gene ( CYP4G15 ) whose drive differences natural population. transiently upregulated DENV-resistant midguts, while knockdown increases susceptibility, and transgenic overexpression enhances resistance....
ABSTRACT Arthropod-borne viruses (arboviruses) such as dengue virus (DENV) and Zika (ZIKV) pose a significant threat to global health. Novel approaches control the spread of arboviruses focus on harnessing antiviral immune system their primary vector, Aedes aegypti mosquito. In arthropods, genes Vago family are often presented analogs mammalian cytokines with potential functions, but role upon infection in Ae. is largely unknown. We conducted phylogenetic analysis gene Diptera, which led us...
Summary Endogenous viral elements (EVEs) are sequences integrated in host genomes. A large number of non-retroviral EVEs was recently detected Aedes mosquito genomes, leading to the hypothesis that may control exogenous infections by closely related viruses. Here, we experimentally investigated role an EVE naturally found aegypti populations and derived from widespread insect-specific virus, cell-fusing agent virus (CFAV). Using CRISPR/Cas9 genome editing, created Ae. line lacking CFAV EVE....
The deleterious effects of inbreeding have been extreme importance to evolutionary biology, but it has difficult characterize the complex interactions between genetic constraints and selection that lead fitness loss recovery after inbreeding. Haploid organisms selfing like nematode Caenorhabditis elegans are capable rapid from fixation novel mutation; however, potential for genomic consequences in diploid, outcrossing not well understood. We sought answer two questions: 1) Can a population...
Abstract Transposable elements (TEs) are DNA sequences that can change their position within a genome. In the germline of arthropods, post-transcriptional regulation TE expression is mainly mediated by Piwi-interacting RNA (piRNA) pathway. piRNAs small RNAs 24-30 nucleotides (nt) in length produced from genomic precursor transcripts as well through ‘ping-pong’ amplification cycle. somatic tissues, certain insects, such Drosophila , instead rely on interfering (siRNA) pathway key regulator...
Abstract Arthropod-borne viruses (arboviruses) transmitted by Aedes aegypti mosquitoes are an increasing threat to global health. The small interfering RNA (siRNA) pathway is considered the main antiviral immune of insects, and thus represents a potential key target in development novel transmission-blocking strategies against arboviruses. Although function siRNA Ae. well established, its effective impact on arbovirus transmission surprisingly poorly understood. Here, we used...