A5039512707- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Bacterial Identification and Susceptibility Testing
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Orthopaedic implants and arthroplasty
- Animal Virus Infections Studies
- Crystallization and Solubility Studies
- Orthopedic Infections and Treatments
- X-ray Diffraction in Crystallography
- Total Knee Arthroplasty Outcomes
- Antimicrobial Resistance in Staphylococcus
- Mycobacterium research and diagnosis
- Streptococcal Infections and Treatments
- Respiratory viral infections research
- Cardiac, Anesthesia and Surgical Outcomes
- Malaria Research and Control
- Antibiotic Resistance in Bacteria
- Infective Endocarditis Diagnosis and Management
- Clostridium difficile and Clostridium perfringens research
- Diphtheria, Corynebacterium, and Tetanus
- Amoebic Infections and Treatments
- Syphilis Diagnosis and Treatment
- Tuberculosis Research and Epidemiology
Azienda Unità Sanitaria Locale Della Romagna
2017-2024
Policlinico S.Orsola-Malpighi
2020
Clinical Orthopaedics and Related Research
2018
Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda
2011
Diagnostic tools that can rapidly identify and characterize microbes growing in blood cultures are important components of clinical microbiology practice because they help to provide timely information be used optimize patient management. This publication describes the bioMérieux BIOFIRE Blood Culture Identification 2 (BCID2) Panel study was submitted U.S. Food & Drug Administration. Results obtained with BCID2 were compared standard-of-care (SoC) results, sequencing PCR reference laboratory...
Prosthetic joint infection (PJI) is among the most-severe complications of a total arthroplasty. Identification causal organism paramount importance for successful treatment, and sonication implants may aid in this identification. Dithiothreitol (DTT) treatment has been proposed as an alternative to improve diagnosis, reduce costs, reliability procedure, but its efficacy remains poorly characterized.(1) Are DTT more sensitive and/or specific than standard cultures tissue samples diagnosis...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified in Wuhan, China, late 2019 and is the causative agent of disease (COVID-19) pandemic. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) represents gold standard for diagnostic assays even if it cannot precisely quantify viral RNA copies. Thus, we decided to compare qRT-PCR with digital (dPCR), which able give an accurate number copies that can be found a specimen. However, aforementioned...
The continuous emergence of SARS-CoV-2 variants and subvariants poses significant public health challenges. latest designated subvariant JN.1, with all its descendants, shows more than 30 mutations in the spike gene. JN.1 has raised concerns due to genomic diversity potential enhance transmissibility immune evasion. This study aims analyse molecular characteristics JN.1-related lineages (JN.1*) identified Italy from October 2023 April 2024 evaluate neutralization activity against a subsample...
The antibiotic ciprofloxacin (CIP) zwitterion has been cocrystallized via slurry and/or ball-milling with carvacrol (CAR) and thymol (THY), also known to exert antimicrobial activity, the aim of improving antibacterial activity ciprofloxacin. In case CAR, 1:4 cocrystal CIP·CAR4 appears be most stable phase, where intermediate phases CIP·CAR3 CIP·CAR2 have isolated by stepwise loss CAR. THY, 1:2 CIP·THY2 is stable, while CIP·THY4 easily loses THY yield bisadduct. All cocrystals were...
Literature offers plenty of cases immunocompromised patients, who develop chronic and severe SARS-CoV-2 infections. The aim this study is to provide further insight into evolutionary dynamic taking exam a subject suffering from follicular lymphoma, developed persistent infection for over 7 months. Eight nasopharyngeal swabs were obtained, analyses by qRT-PCR diagnostic purposes. All them considered eligible (Ct < 30) NGS sequencing. Sequence analysis showed that all sequences matched the...
The recent emergence of a number new SARS-CoV-2 variants resulting from recombination between two distinct parental lineages or sub-lineages within the same lineage has sparked debate regarding potential enhanced viral infectivity and immune escape. Among these, XBB, recombinant BA.2.10 BA.2.75, caused major concern in some countries due to its rapid increase prevalence. In this study, we tested XBB escape capacity mRNA-vaccine-induced (BNT162b2) neutralising antibodies compared B.1...
Recombination related to coinfection is a huge driving force in determining the virus genetic variability, particularly conditions of partial immune control, leading prolonged infection. Here, we characterized distinctive mutational pattern, highly suggestive Delta-Omicron double infection, lymphoma patient.The specimen was through combined approach, analyzing results deep sequencing primary sample, viral culture, and plaque assay.Bioinformatic analysis on sequences deriving from sample...
Candida spp. are an important opportunistic pathogen that can represent a possible cause of severe infections, especially in immunocompromised individuals. The clinical impact depends, part, on the ability to form biofilms, communities nestled cells into extracellular matrix. In this study, we compared biofilm formation 83 strains isolated from blood cultures and other materials, such as respiratory samples, urine, exudate, their sensitivity fluconazole (FLZ). Strains were divided tertiles...
The freshwater environment is suitable for nontuberculous mycobacteria (NTMs) growth. Their high adaptability represents a considerable risk sanitary water systems, which are potential vector NTMs transmission. This study investigated the occurrence of NTMs, such as
Bloodstream infection (BSI) and associated sepsis represent a major source of mortality in industrialized countries. Prompt treatment with targeted antibiotics affects both the financial impact clinical outcome BSI: every hour gained initiating correct antimicrobial therapy significantly increases probability patient survival. However, current standard-of-care, which depends on blood culture-based diagnosis, are often unable to provide such fast response. Fast sensitive molecular techniques...
Background: The objective of this study was to validate the Simplified Acute Physiology Score SAPS 3 Admission (SAPS 3) and compare its fit with that II in an independent sample patients admitted a single‐centre intensive care unit (ICU). Methods: data for all adult consecutively eight‐bed ICU 700‐bed university hospital between 1 January 2006 2 September 2007 were collected. computed, as well predicted mortality. calibration 3, according general equation (GE), equations Southern Europe...
The spread of carbapenem-resistant Enterobacteriaceae (CRE) has been enabled by the lack control measures directed at carriers multidrug-resistant organisms in healthcare settings. Screening patients for asymptomatic colonization on one hand, and implementation contact precautions other reduces patient-to-patient transmission. plates represents a relatively low-cost method isolating CRE from rectal swabs; however, molecular assays have become widely available. This study compared performance...
Since the first SARS-CoV-2 outbreak, mutations such as single nucleotide polymorphisms (SNPs) and insertion/deletions (INDELs) have changed characterized viral genome sequence, structure protein folding leading to onset of new variants. The presence those alterations challenges not only clinical field but also diagnostic demand due failures in gene detection or incompleteness polymerase chain reaction (PCR) results. In particular, analysis understudied genes N investigation through...
Zika virus was discovered in 1947. The first reported case of fever a sentinel rhesus monkey Uganda 1947, while the human cases were Nigeria 1954. Since evidence infection, active several countries Africa and Asia, as sporadic serological infections have been demonstrated reports. outbreak Yap Island 2007 is considered emergency this infection. then has spread worldwide with large ongoing epidemic South Central America. A huge concern nowadays about relationship between infection...
Abstract Background The BioFire® FilmArray® Blood Culture Identification 2 (BCID2) Panel is a diagnostic test that provides results for 26 bacterial, 7 fungal pathogens and 10 antimicrobial resistance (AMR) genes from positive blood culture (PBC) specimens in about an hour. BCID2 builds upon the existing BCID with several additional assays include Candida auris expanded AMR gene menu methicillin-resistant Staphylococcus aureus (MRSA) plus detection mcr-1, carbapenem resistance, ESBL. Here,...
Background and aims. Bacterial vaginosis (BV) is one the more frequently identified genital syndrome among childbearing aged women. The basic condition that generates this a modification in vaginal microbiota. aim of paper to briefly review current status art BV report results pilot study performed with an innovative PCR based technique. Materials Methods. 36 samples fluid routinely submitted for diagnosis Unit Microbiology – GRHL were comparatively evaluated by standard techniques...