A5089849160- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Infectious Diseases and Tuberculosis
- Diagnosis and treatment of tuberculosis
- Cell Image Analysis Techniques
- Image Processing Techniques and Applications
- COVID-19 diagnosis using AI
- Legionella and Acanthamoeba research
- Biosensors and Analytical Detection
- Pneumonia and Respiratory Infections
- Infectious Diseases and Mycology
- Cancer therapeutics and mechanisms
- Bacterial Identification and Susceptibility Testing
- Vibrio bacteria research studies
- Synthesis of β-Lactam Compounds
- Pneumocystis jirovecii pneumonia detection and treatment
- Hematological disorders and diagnostics
- Advanced Chemical Sensor Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- COVID-19 Clinical Research Studies
- Digital Imaging for Blood Diseases
- Antibiotic Resistance in Bacteria
- Medical Imaging Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
Azienda USL di Bologna
2021-2025
University of Bologna
2017-2025
Policlinico S.Orsola-Malpighi
2019-2023
Background Diagnosis of pulmonary (PTB) and extra-pulmonary tuberculosis (EPTB) in smear-negative patients can be difficult. We assessed retrospectively the performance Xpert MTB/RIF system (Xpert, Cepheid) diagnosing (TB), which represents most common form TB a low incidence setting. Methods Performance was compared to acid-fast microscopic examination using Ziehl-Neelsen (ZN) stain with culture-confirmed TB. Results 386 Mycobacterium (MTB) culture-positive samples were detected out 5170...
Background The new Xpert MTB/RIF Ultra assay (Ultra, Cepheid, Sunnyvale, USA) is a cartridge-based automated diagnostic test that can simultaneously identify Mycobacterium tuberculosis complex (MTB) and resistance to Rifampicin (RIF). With respect the previous version (Xpert), IS6110/IS1081 repetitive elements probes have been added allowing detection of lower MTB load, defined by semi-quantitative category "trace" with indeterminate RIF resistance. aim this study was evaluate performance on...
Tuberculosis (TB), caused by the Mycobacterium tuberculosis complex (MTBC), remains a major global health challenge. Whole genome sequencing (WGS) offers an invaluable tool for understanding genetic diversity and drug resistance profiles of MTBC. This study provides comprehensive WGS dataset 2,520 MTBC isolates collected from four Italian regions-Lombardy, Piedmont, Emilia-Romagna, Lazio-between 2017 2020. The includes genomic data along with associated metadata, such as geographic location...
Legionella spp. are widespread bacteria in aquatic environments with a growing impact on human health. Between the 61 species, pneumophila is most prevalent diseases; contrary, non- species less detected clinical diagnosis or during environmental surveillance due to their slow growth culture and absence of specific rapid diagnostic/analytical tools. Reliable isolate identification essential estimate source infection, undertake containment measures, determine treatment. Matrix-assisted laser...
Legionella spp. are Gram-negative bacteria that inhabit freshwater environments representing a serious risk for human health. pneumophila ( Lp ) is the species most frequently responsible severe pneumonia known as Legionnaires' disease. consists of 15 serogroups (Sgs), usually identified by monoclonal or polyclonal antibodies. With regard to serogrouping, it well phenotyping methods do not have sufficiently high discriminating power, while genotypic although very effective, expensive and...
Mycobacterium chimaera is ubiquitously spread in the environment, including factory and hospital water systems. Invasive cases of M. infection have been associated with aerosols produced by use heater-cooler units (HCU) during cardiac surgery. The aim this study was to evaluate for first time performance IR-Biotyper system on a large number isolates collected from longitudinal environmental HCUs samples sources hospitals located three Italian provinces. In addition, results were compared...
The freshwater environment is suitable for nontuberculous mycobacteria (NTMs) growth. Their high adaptability represents a considerable risk sanitary water systems, which are potential vector NTMs transmission. This study investigated the occurrence of NTMs, such as
Background Clinical interpretation of trace results by Xpert MTB/RIF Ultra assay (Ultra) used as an initial diagnostic test for tuberculosis (TB) may be challenging. The aim the study was to evaluate frequency and epidemiology readouts in routine clinical practice a low TB prevalence setting propose guidance on how manage patients with calls considering data available (clinical, radiological, bacteriological etc.). Materials methods A retrospective, observational, monocentric conducted at...
QuantiFERON-TB Gold Plus (QFT-Plus) is the most widely used interferon gamma release assay (IGRA) for diagnosis of latent tuberculosis infection (LTBI). The aim this study was to compare QFT-Plus results by enzyme-linked immunosorbent (ELISA) on SkyLab system with those obtained chemiluminescence immunoassay (CLIA) Liaison XL analyzer. Agreement between two assays evaluated 419 blood samples and found be substantial (75.4%); higher agreement positive (95.
The most commonly used method for the detection of non-tuberculous mycobacteria (NTM) is culture in BD BACTEC™ MGIT™ Mycobacteria Growth Indicator Tubes incubated an automated growth reader 960 Instrument. system currently validated from clinical specimens but not environmental matrices.
Effective treatment of tuberculosis is frequently hindered by the emerging antimicrobial resistance Mycobacterium tuberculosis. The present study evaluates monocyclic β-lactam compounds targeting mycobacterial cell wall remodeling. Novel N-thio-β-lactams were designed, synthesized, and characterized on L,D-transpeptidase-2, a validated target in M. candidates evaluated biochemical assays identifying five presenting target-specific kinetic constants equal or superior to meropenem, carbapenem...
Mycobacterium saskatchewanense is a species of pigmented slow-growing Non-Tuberculous Mycobacteria (NTM), positive for avium complex (MAC) by AccuProbe system. MAC organisms have frequently been isolated from different medical devices. This the first study reporting isolation M. devices and highlights importance correctly identifying NTMs that often colonize sanitary water. GenoType CM CE-IVD kit (CM) was used as step NTM strain identification, all cultures were found to be components MAC....
The MDR/MTB ELITe MGB® Kit on the InGenius® platform (ELITechGroup SpA, Italy) is first system for simultaneous detection of Mycobacterium tuberculosis complex (MTBc) genome and main mutations responsible resistance to Isoniazid (inhA, katG) Rifampicin (rpoB), from decontaminated heat inactivated samples. In this study we compared performance (ELITe) with culture in 100 pulmonary 160 extra-pulmonary sensitivity specificity samples were 98.0% respectively; overall was 86.3% (80% urine, 85%...
Mycobacterium chimaera, belonging to the avium complex, is an opportunistic environmental mycobacterium which has been isolated from medical device water samples such as Heater Cooler Units (HCU). Laboratories currently use culture-based diagnostic methods detect M. but these take a long time obtain results. The aim of this study test and define Limit Detection (LoD) Real-Time Polymerase Chain Reaction (RT-PCR) specific for using sample-to-result InGenius platform, performed on collected at...
Abstract Extra-pulmonary mycobacterial infections are characterized by a paucibacillary nature and extra-pulmonary samples consist of different matrices; the processing these requires high level manual skills non-standardized procedures. The aim this study was to compare performance MYCO-TB with MycoPrep on in terms Mycobacteria detection, culture contamination suitability for molecular assay. This prospective conducted 201 from suspected cases infection. Specimens were divided into two...
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