A5066267303- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Biosensors and Analytical Detection
- Animal Virus Infections Studies
- Inhalation and Respiratory Drug Delivery
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Bacillus and Francisella bacterial research
- Zoonotic diseases and public health
- vaccines and immunoinformatics approaches
- CRISPR and Genetic Engineering
- Viral Infections and Immunology Research
- Antimicrobial Resistance in Staphylococcus
- COVID-19 diagnosis using AI
- Syphilis Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Plasma Applications and Diagnostics
- Virus-based gene therapy research
- Microbial Metabolism and Applications
- Bacterial biofilms and quorum sensing
- Infection Control and Ventilation
- Orthopaedic implants and arthroplasty
- Conducting polymers and applications
- Pneumocystis jirovecii pneumonia detection and treatment
Azienda Unità Sanitaria Locale Della Romagna
2021-2024
University of Bologna
2024
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified in Wuhan, China, late 2019 and is the causative agent of disease (COVID-19) pandemic. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) represents gold standard for diagnostic assays even if it cannot precisely quantify viral RNA copies. Thus, we decided to compare qRT-PCR with digital (dPCR), which able give an accurate number copies that can be found a specimen. However, aforementioned...
Literature offers plenty of cases immunocompromised patients, who develop chronic and severe SARS-CoV-2 infections. The aim this study is to provide further insight into evolutionary dynamic taking exam a subject suffering from follicular lymphoma, developed persistent infection for over 7 months. Eight nasopharyngeal swabs were obtained, analyses by qRT-PCR diagnostic purposes. All them considered eligible (Ct < 30) NGS sequencing. Sequence analysis showed that all sequences matched the...
The recent emergence of a number new SARS-CoV-2 variants resulting from recombination between two distinct parental lineages or sub-lineages within the same lineage has sparked debate regarding potential enhanced viral infectivity and immune escape. Among these, XBB, recombinant BA.2.10 BA.2.75, caused major concern in some countries due to its rapid increase prevalence. In this study, we tested XBB escape capacity mRNA-vaccine-induced (BNT162b2) neutralising antibodies compared B.1...
Recombination related to coinfection is a huge driving force in determining the virus genetic variability, particularly conditions of partial immune control, leading prolonged infection. Here, we characterized distinctive mutational pattern, highly suggestive Delta-Omicron double infection, lymphoma patient.The specimen was through combined approach, analyzing results deep sequencing primary sample, viral culture, and plaque assay.Bioinformatic analysis on sequences deriving from sample...
Candida spp. are an important opportunistic pathogen that can represent a possible cause of severe infections, especially in immunocompromised individuals. The clinical impact depends, part, on the ability to form biofilms, communities nestled cells into extracellular matrix. In this study, we compared biofilm formation 83 strains isolated from blood cultures and other materials, such as respiratory samples, urine, exudate, their sensitivity fluconazole (FLZ). Strains were divided tertiles...
Abstract Bioaerosols (aerosolized particles with biological origin) are strongly suspected to play a significant role in the transmission of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2), especially closed indoor environments. Thus, control technologies capable effectively inactivating bioaerosols urgently needed. In this regard, cold atmospheric pressure plasma (CAP) can represent suitable option, thanks its ability produce reactive species, which exert antimicrobial action....
Abstract Due to the SARS-CoV-2 pandemic renewed attention has been directed towards viral neutralization assays and neutralizing antibodies quantification, for vaccine pre-clinical trials determining efficacy over time. The gold standard assess antibody titer is plaque reduction test, an end-point assay which evaluates highest serum dilution that neutralizes replication, by inspecting cytopathic effect induced on cell cultures. Here, we use planar, PEDOT:PSS-based organic electrochemical...
Introduction: Serratia ficaria, is a Gram-negative rod first identified in 1979 the fig tree ecosystem. Since then, ficaria has been occasionally isolated from human specimens. According to literature, an opportunistic pathogen that rarely affects human, when infection occurs it can lead different clinical conditions, including gallbladder empyema, lithiasis, ophthalmitis, leg ulceration, respiratory infections. Case presentation: We report case of 66-year-old woman whom was causative agent...
In-vitro viral studies are still fundamental for biomedical research since studying the virus kinetics on cells is crucial determination of biological properties viruses and screening inhibitors infections. Moreover, testing potential contaminants often mandatory safety evaluation. Nowadays, cytopathic effects mainly evaluated through end-point assays requiring dye-staining combined with optical Recently, optical-based automatized equipment has been marketed, aimed at real-time cell-layer...
This work illustrates the development of a dry inhalation powder cyclosporine-A for prevention rejection after lung transplantation and treatment COVID-19. The influence excipients on spray-dried powder's critical quality attributes was explored. best-performing in terms dissolution time respirability obtained starting from concentration ethanol 45% (v/v) feedstock solution 20% (w/w) mannitol. showed faster profile (Weibull 59.5 min) than poorly soluble raw material (169.0 min). exhibited...
Since the first SARS-CoV-2 outbreak, mutations such as single nucleotide polymorphisms (SNPs) and insertion/deletions (INDELs) have changed characterized viral genome sequence, structure protein folding leading to onset of new variants. The presence those alterations challenges not only clinical field but also diagnostic demand due failures in gene detection or incompleteness polymerase chain reaction (PCR) results. In particular, analysis understudied genes N investigation through...
Objectives To exploit the features of digital PCR for implementing SARS-CoV-2 observational studies by reliably including viral load factor expressed as copies/μL. Methods A small cohort 51 Covid-19 positive samples was assessed both RT-qPCR and assays. linear regression model built using a training subset, its accuracy in remaining evaluation subset. The then used to convert stored cycle threshold values large dataset 6208 diagnostic into copies/μL SARS-CoV-2. calculated single...
Abstract The ongoing evolution of SARS-CoV-2 and the emergence new viral variants bearing specific escape mutations responsible for immune evasion from antibody neutralisation has required a more accurate characterisation response as one evolutive forces behind adaptation to largely immunised human population. In this work, culturing in presence neutralising sera vigorously promoted mutagenesis leading acquisition known on spike well presumptive structural proteins whose role target...
Sexually transmitted infections (STIs) have seen a considerable increase in the last years and given health burden they may represent from both personal community perspective, require surveillance prevention programmes based on timely decentralized diagnosis. In this context, user-friendly rapid molecular tests good trade-off between diagnostic accuracy, accessibility affordability. study we evaluated performance of new real-time LAMP (Loop Mediated Isothermal Amplification) method for...
, identified in 2006, represents a challenging case of bacterial taxonomic identification because its high similarity to
Since the beginning of pandemic, SARS-CoV-2 has caused problems for all world’s population, not only in terms deaths but also overloading healthcare facilities countries. Diagnosis is one key aspects controlling spread SARS-CoV-2, and among current molecular techniques, real-time PCR considered as gold standard. The availability tests that allow rapid accurate identification therefore considerable importance. Moreover, if these even minimal intervention by operator, any risk contamination...
Background: Antigen testing has become an essential part of fighting the ongoing COVID-19 pandemic. With constant increase in available tests, independent extensive comparative evaluations and data from external quality assessment (EQA) studies to evaluate test performance amongst different users are still missing.Objectives: An EQA scheme been established assess sensitivity antigen tests potential impact circulating SARS-CoV-2 strains on their performance.Study design: Panels have prepared...
Mutations in the receptor binding domain (RBD) of SARS-CoV-2 alter infectivity, pathogenicity, and transmissibility new variants concern (VOCs). In addition, those mutations cause immune escape, undermining population immunity induced by ongoing mass vaccination programs. There is an urgent need for novel strategies techniques aimed at surveillance active emergence spread VOCs. The aim this study was to provide a quick, cheap straightforward denaturing high-performance liquid chromatography...
Abstract Introduction Mutations in the receptor binding domain (RBD) region of SARS-CoV-2 have been shown to impact infectivity, pathogenicity and transmissibility new variants concern (VOC). Even more worrisome, those mutations potential causing immune escape, undermining population immunity induced by ongoing mass vaccination programs. Gap statement The massive parallel sequencing techniques taken a lead role detection strategies variants. Nevertheless, they are still cumbersome...