A5010388051- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Diabetes and associated disorders
- Viral gastroenteritis research and epidemiology
- Pancreatic function and diabetes
- Bacterial Identification and Susceptibility Testing
- Pneumonia and Respiratory Infections
- COVID-19 diagnosis using AI
- Viral Infections and Vectors
- Tuberculosis Research and Epidemiology
- Streptococcal Infections and Treatments
- COVID-19 Clinical Research Studies
- Biosensors and Analytical Detection
- Influenza Virus Research Studies
- Herpesvirus Infections and Treatments
- COVID-19 epidemiological studies
- Diabetes Management and Research
- Immune Cell Function and Interaction
- Antibiotic Use and Resistance
- Poxvirus research and outbreaks
- Nosocomial Infections in ICU
- Cytomegalovirus and herpesvirus research
- Diphtheria, Corynebacterium, and Tetanus
- Bacillus and Francisella bacterial research
Columbia University Irving Medical Center
2022-2025
Northwell Health
2017-2024
Donald & Barbara Zucker School of Medicine at Hofstra/Northwell
2019-2024
Columbia University
2024
New York Hospital Queens
2023
Hofstra University
2017-2022
Museum of Heilongjiang Province
2021
The University of Texas Medical Branch at Galveston
2015-2018
American Society for Microbiology
2016
Pennsylvania State University
2013-2015
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has now spread across the globe. As part of worldwide response, many molecular diagnostic platforms have been granted emergency use authorization (EUA) by Food and Drug Administration (FDA) to identify SARS-CoV-2 positive patients. Our objective was evaluate three sample-to-answer (Cepheid Xpert Xpress [Xpert Xpress], Abbott ID NOW COVID-19 [ID NOW], GenMark ePlex Test [ePlex]) determine analytical sensitivity, clinical...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the novel human that causes disease 2019 (COVID-19), was first discovered in December as cause of an outbreak pneumonia city Wuhan, Hubei province, China. The clinical presentation COVID-19 is fairly nonspecific, and symptoms overlap those other seasonal infections concurrently circulating population. Furthermore, it estimated up to 80% infected individuals experience mild or are asymptomatic, confounding efforts reliably diagnose...
With the approach of respiratory virus season in Northern Hemisphere, clinical microbiology and public health laboratories will need rapid diagnostic assays to distinguish severe acute syndrome coronavirus 2 (SARS-CoV-2) from influenza syncytial (RSV) infections for diagnosis surveillance. In this study, performance Xpert Xpress SARS-CoV-2/Flu/RSV test (Cepheid, Sunnyvale, CA, USA) nasopharyngeal swab specimens was evaluated four centers: Johns Hopkins Medical Microbiology Laboratory,...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified in December 2019 and has quickly become a worldwide pandemic. In response, many diagnostic manufacturers have developed molecular assays for SARS-CoV-2 under the Food Drug Administration (FDA) Emergency Use Authorization (EUA) pathway. This study compared three of these assays, Hologic Panther Fusion assay (Fusion), Aptima (Aptima), BioFire Defense COVID-19 test (BioFire), to determine analytical clinical...
Diagnostic tools that can rapidly identify and characterize microbes growing in blood cultures are important components of clinical microbiology practice because they help to provide timely information be used optimize patient management. This publication describes the bioMérieux BIOFIRE Blood Culture Identification 2 (BCID2) Panel study was submitted U.S. Food & Drug Administration. Results obtained with BCID2 were compared standard-of-care (SoC) results, sequencing PCR reference laboratory...
While diagnosis of COVID-19 relies on qualitative molecular testing for the absence or presence SARS-CoV-2 RNA, quantitative viral load determination has many potential applications in antiviral therapy and vaccine trials as well implications public health quarantine guidance. To date, no tests have been authorized clinical use by FDA. In this study, we modified FDA emergency RealTime assay into a Laboratory Developed Test (LDT) using newly developed Abbott calibration standards. Both...
ABSTRACT Accurate and rapid antimicrobial susceptibility test (AST) results from positive blood cultures are crucial for patient care combatting resistance. Although recent advancements in direct-from-positive culture (PBC) identification platforms have enabled the provision of species-level some resistance marker information within hours after positivity, AST required clinical decision-making often require 48 h positivity. This study evaluated Selux next-generation phenotyping system,...
An accurate and timely diagnosis of pharyngitis caused by group A streptococcus (GAS) is essential for ensuring appropriate antibiotic therapy. Early detection through point-of-care (POC) testing valuable in initiating effective treatment. This study aimed at evaluating the analytical sensitivity molecular ID NOW™ Strep 2 POC test as compared to 3 lateral flow assays: BD Veritor™ Plus System, Sofia® A+ Fluorescent Immunoassay, Sekisui Diagnostic OSOM® GAS detection. Two Streptococcus...
Abstract Background In March 2020, the greater New York metropolitan area became an epicenter for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The initial evolution of case incidence has not been well characterized. Methods Northwell Health Laboratories tested 46 793 persons SARS-CoV-2 from 4 through 10 April. primary outcome measure was a positive reverse transcription–polymerase chain reaction test SARS-CoV-2. secondary outcomes included patient age, sex, and...
Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes disease 2019 (COVID-19), can be detected in samples by real-time reverse transcriptase polymerase chain reaction (RT-PCR) or other molecular methods. Accessibility of diagnostic testing for COVID-19 has been limited intermittent shortages supplies required testing, including flocked nasopharyngeal (FLNP) swabs. Methods We developed a 3-dimensional printed (3DP) swab as replacement FLNP...
As the incidence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) begins to overlap with traditional season in Northern Hemisphere, simultaneous testing for SARS-CoV-2 and other common causes infections is imperative. This has led development multiplex assays that include as a target. One such assay BioFire panel 2.1 (RP2.1), which an expansion original FilmArray (RP2) SARS-CoV-2. In this multicenter evaluation, we assessed performance characteristics RP2.1 detection or more...
Rapid detection of group A Streptococcus (GAS) is an integral component treatment decisions in the clinic, especially pediatric population. We prospectively collected 216 specimens from symptomatic, predominantly patients and evaluated performance Alere i Strep test (Alere i; Inc., Scarborough, ME) BD Veritor system (BD Veritor; Becton, Dickinson Company, Sparks, MD), with culture results being used as a comparator. Real-time PCR (RT-PCR) was performed arbiter discordant cases. Comprehensive...
The rapid emergence of the coronavirus disease 2019 (COVID-19) pandemic has introduced a new challenge in diagnosing and differentiating respiratory infections. Accurate diagnosis infections, including severe acute syndrome 2 (SARS-CoV-2), is complicated by overlapping symptomology, stepwise approaches to testing for each infection would lead increased reagent usage cost, as well delays clinical interventions. To avoid these issues, multiplex molecular assays have been developed...
Mutations in the genome of SARS-CoV-2 can affect performance molecular diagnostic assays. In some cases, such as S-gene target failure, impact serve a unique indicator particular variant and provide method for rapid detection.
The nonobese diabetic (NOD) mouse spontaneously develops autoimmune diabetes after 12 weeks of age and is the most extensively studied animal model human Type 1 (T1D). Cell transfer studies in irradiated recipient mice have established that T cells are pivotal T1D pathogenesis this model. We describe herein a simple method to rapidly induce by adoptive purified, primary CD4+ from pre-diabetic NOD transgenic for islet-specific cell receptor (TCR) BDC2.5 into NOD.SCID mice. major advantages...
NOD.B10 Idd9.3 mice are congenic for the insulin‐dependent diabetes ( Idd ) locus, which confers significant type 1 (T1D) protection and encodes 19 genes, including microRNA (miR)‐34a, from T1D‐resistant C57BL/10 mice. B cells have been shown to play a critical role in priming of autoantigen‐specific CD4 + T T1D pathogenesis non‐obese diabetic (NOD) We show that early B‐cell development is impaired mice, resulting profound reduction transitional mature splenic as compared with NOD Molecular...
COVID-19 is associated with prolonged hospitalization and a high risk of intubation, which raises concern for bacterial coinfection antimicrobial resistance. Previous research has shown wide range pneumonia rates patients in variety clinical demographic settings, but none have compared hospitalized to testing negative severe acute respiratory syndrome coronavirus (SARS-CoV-2) similar care settings.
Our reverse syphilis testing algorithm consists of a treponemal IgG multiplex flow immunoassay (MFI) followed by both rapid plasma reagin titer and the Treponema pallidum particle agglutination (TPPA) test on specimens with reactive MFI result. We report here impact modified algorithm, in which strength signal is used to avoid unnecessary TPPA testing.The Bioplex was as screening assay, equivocal (antibody index 0.9 or 1.0), ≥ 1.1) results were further tested test. performed retrospective,...
An epidemic caused by an outbreak of mpox (formerly monkeypox) in May 2022 rapidly spread internationally, requiring urgent response from the clinical diagnostics community. A detailed description validation and implementation a laboratory-developed real-time PCR test for detecting nonvariola Orthopoxvirus-specific DNA based on newly designed RealStar Zoonotic Orthopoxvirus assay is presented. The was performed using accuracy panel (n = 97) comprising skin lesion swabs universal transport...
Abstract Background and Objectives Platelet transfusions are increasing with medical advances. Based on FDA criteria, platelet units assessed by in vitro measures; however, it is not known how processing storage duration affect function vivo. Our study's aim was to develop a novel transfusion model stored mouse plasma that meets criteria adapted mice, transfused fresh platelets detectable clots Study Design Methods were prepared using modified platelet‐rich (PRP) collection protocol....
ABSTRACT Bloodstream HSV-1 and HSV-2 infections can cause devastating outcomes with high morbidity mortality, especially in neonates or immunocompromised individuals. Proper patient management for herpes simplex virus (HSV) bloodstream is time-sensitive requires a rapid, accurate, definitive diagnosis. The absence of the U.S. Food Drug Administration (FDA)-approved molecular assays HSV detection blood, coupled lack consensus on optimal sample type, underscores unmet need improved...
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