A5028577117- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- Cardiac and Coronary Surgery Techniques
- Angiogenesis and VEGF in Cancer
- Cardiac Valve Diseases and Treatments
- Congenital heart defects research
- Mesenchymal stem cell research
- Hippo pathway signaling and YAP/TAZ
- Cellular Mechanics and Interactions
- Renal and related cancers
- Animal Genetics and Reproduction
- Cardiac Fibrosis and Remodeling
- Coronary Interventions and Diagnostics
- Reproductive Biology and Fertility
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Zebrafish Biomedical Research Applications
- COVID-19 Clinical Research Studies
- Sperm and Testicular Function
- Cardiomyopathy and Myosin Studies
- Mechanical Circulatory Support Devices
- Cardiac Ischemia and Reperfusion
- Epigenetics and DNA Methylation
- Genetics, Aging, and Longevity in Model Organisms
Centro Cardiologico Monzino
2016-2025
King Faisal Specialist Hospital & Research Centre
2024-2025
Polytechnic University of Turin
2025
Istituti di Ricovero e Cura a Carattere Scientifico
2009-2024
Mylan (Switzerland)
2017-2020
Gruppo CLAS (Italy)
2019
Bioengineering Center
2016
National Center for Genetic Engineering and Biotechnology
2016
Synthetic Biologics (United States)
2016
Evolutionary Genomics (United States)
2015
The POU transcription factor Oct-4 is expressed in early mouse embryogenesis and pluripotent embryonal stem (ES) carcinoma (EC) cell lines. After gastrulation the embryo, expression confined to germline. present study provides evidence that undergoes downregulation during oogenesis spermatogenesis, coincident with entry into meiosis. Furthermore, analysis of maturation stages oocytes showed upregulated de novo final meiotic prophase I female germ cells. These data suggest cells both sexes...
Abstract Long COVID has become a world-wide, non-communicable epidemic, caused by long-lasting multiorgan symptoms that endure for weeks or months after SARS-CoV-2 infection already subsided. This scientific document aims to provide insight into the possible causes and therapeutic options available cardiovascular manifestations of long COVID. In addition chronic fatigue, which is common symptom COVID, patients may present with chest pain, ECG abnormalities, postural orthostatic tachycardia,...
ABSTRACT Proliferating primordial germ cells (PGCs) isolated from mouse embryos soon after their arrival in the genital ridges would only survive vitro at temperature of less than 30°C (De Felici, M. and McLaren, A. (1983). Exp. Cell. Res. 144, 417 –427; Wabik-Sliz, B. (1984). 154, 530 –536) or when co-cultured on cell feeder layers (Donovan, P. J., Stott, D., Godin, I., Heasman, J. Wylie, C. (1986). Cell 44, 831–838; De Dolci, S. (1991). Dev. Biol. 147, 281– 284). In present paper we report...
Human umbilical cord blood (UCB) contains high numbers of endothelial progenitors cells (EPCs) characterized by coexpression CD34 and CD133 markers. Prior studies have shown that + /CD133 EPCs from the or peripheral (PB) can give rise to induce angiogenesis in ischemic tissues. In present study, it is freshly isolated human injected into adductor muscles gave and, unexpectedly, skeletal muscle mice. fact, treated limbs exhibited enhanced arteriole length density regenerating fiber density....
The Oct-4 transcription factor, a member of the POU family that is also known as Oct-3 and Oct3/4, expressed in totipotent embryonic stem cells (ES) germ cells, it has unique role development determination pluripotency. ES may have their postnatal counterpart adult recently described various mammalian tissues, expression putative purified from tissues been considered real marker stemness. In this context, normal mature would not be expected to show expression. On contrary, we demonstrated,...
Abstract Cardiovascular diseases represent a major cause of morbidity and mortality, necessitating research to improve diagnostics, discover test novel preventive curative therapies, all which warrant experimental models that recapitulate human disease. The translation basic science results clinical practice is challenging task, in particular for complex conditions such as cardiovascular diseases, often result from multiple risk factors comorbidities. This difficulty might lead some...
Background: Conversion of cardiac stromal cells into myofibroblasts is typically associated with hypoxia conditions, metabolic insults, and/or inflammation, all which are predisposing factors to fibrosis and heart failure. We hypothesized that this conversion could be also mediated by response these mechanical cues through activation the Hippo transcriptional pathway. The objective present study was assess role cellular/nuclear straining forces acting in myofibroblast differentiation under...
The heart contains c-kit+ progenitors that maintain cardiac homeostasis. Cardiac cells are multipotent and give rise to myocardial, endothelial smooth muscle cells, both in vitro vivo. C-kit+ have been thoroughly investigated for their stem cell activity, susceptibility stress conditions ageing, as well ability repair the infarcted heart. Recently, expression of mesenchymal (MSC) markers MSC differentiation potency reported progenitor cells. Based on this evidence, we hypothesized may...
ABSTRACT Cardiac cell function is substantially influenced by the nature and intensity of mechanical loads cells experience. fibroblasts (CFs) are primarily involved in myocardial tissue remodeling: at onset specific pathological conditions, CFs activate, proliferate, differentiate, critically alter amount extra‐cellular matrix with important consequences for functioning. While cyclic strain has been shown to increase synthesis vitro, role cues proliferation unclear. We here developed a...
Abstract The ‘cardiosphere’ is a 3D cluster of cardiac progenitor cells recapitulating stem cell niche-like microenvironment with potential for disease and regeneration modelling the failing human myocardium. In this multicellular context, it extremely important to decrypt spatial distribution markers dissecting evolution cellular phenotypes by direct quantification fluorescent signals in confocal microscopy. study, we present fully automated method, named CARE (‘CARdiosphere Evaluation’),...