A5059891753- 3D Printing in Biomedical Research
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- Electrospun Nanofibers in Biomedical Applications
- Microfluidic and Bio-sensing Technologies
- Mediterranean and Iberian flora and fauna
- Neuroscience and Neural Engineering
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Immunotherapy and Biomarkers
- Microfluidic and Capillary Electrophoresis Applications
- Botanical Studies and Applications
- Angiogenesis and VEGF in Cancer
- Nanoplatforms for cancer theranostics
- Barrier Structure and Function Studies
- Cytomegalovirus and herpesvirus research
- CRISPR and Genetic Engineering
- Hepatitis B Virus Studies
- Virus-based gene therapy research
- Cancer Research and Treatments
- Glioma Diagnosis and Treatment
- Planarian Biology and Electrostimulation
- Pluripotent Stem Cells Research
Agency for Science, Technology and Research
2017-2025
Institute of Molecular and Cell Biology
2017-2025
National University of Singapore
2023-2025
Nanyang Technological University
2025
Singapore-MIT Alliance for Research and Technology
2013-2019
Singapore Immunology Network
2019
IIT@MIT
2019
Bioengineering Center
2016
National Center for Genetic Engineering and Biotechnology
2016
Synthetic Biologics (United States)
2016
A 3D microfluidic neurovascular model (A), using a differential seeding procedure (B) for co-culturing neurons, astrocytes and endothelial cells (C).
The tumor microenvironment imposes physical and functional constraints on the antitumor efficacy of adoptive T cell immunotherapy. Preclinical testing different preparations can help in selection efficient immune therapies, but vivo models are expensive cumbersome to develop, while classical vitro 2D cannot recapitulate spatiotemporal dynamics experienced by cells targeting cancer. Here, we describe an easily customizable 3D model, which conditions modulated functionality is tested. We...
The oxygen-evolving complex of eukaryotic photosystem II (PSII) consists four extrinsic subunits, PsbO (33 kDa), PsbP (23 PsbQ (17 kDa) and PsbR (10 encoded by seven nuclear genes, PsbO1 (At5g66570), PsbO2 (At3g50820), PsbP1 (At1g06680), PsbP2 (At2g30790), PsbQ1 (At4g21280), PsbQ2 (At4g05180) (At1g79040). Using Arabidopsis insertion mutant lines, we show that PsbP1, but not PsbP2, is essential for photoautotrophic growth, whereas plants lacking both forms and/or normal growth rates. Complete...
In the hepatitis-B virus-related hepatocellular carcinoma tumor microenvironment (TME), monocytes reportedly impede natural T cell functions via PD-L1/PD-1 signaling. However, it remains unclear if receptor (TCR)-redirected cells are similarly inhibited. We thus developed a 3D intrahepatic TME microfluidic model to investigate immunosuppressive potential of toward HBV-specific TCR and role Interestingly, in our static model, we observed that suppressed only retrovirally transduced (Tdx)...
Abstract Stem cell research has yielded promising advances in regenerative medicine, but standard assays generally lack the ability to combine different stimulations with rapid sample processing and precise fluid control. In this work, we describe design fabrication of a micro-scale stimulator capable simultaneously providing mechanical, electrical biochemical stimulation subsequently extracting detailed morphological gene-expression analysis on cellular response. This micro-device offers...
RNA methyltransferase METTL6 is implicated in tumor cell growth and mouse energy consumption.
We developed a computational model to predict oxygen levels in microfluidic plastic devices during cell culture. This is based on experimental evaluation of levels. Conditions are determined that provide adequate supply two types, hepatocytes and endothelial cells, either by diffusion through the device, or supplying low flow rate medium.
Abstract The limitations of current cancer therapies highlight the urgent need for a more effective therapeutic strategy. One promising approach uses an alternating electric field; however, mechanisms involved in disruption cell cycle as well potential adverse effects on non-cancerous cells must be clarified. In this study, we present novel microfluidic device with embedded electrodes that enables application field therapy to 3D extracellular matrix. To demonstrate our system aid designing...
Abstract Glioblastoma (GBM) is the most common primary malignant brain cancer in adults with a dismal prognosis. Temozolomide (TMZ) first‐in‐line chemotherapeutic; however, resistance frequent and multifactorial. While many molecular genetic factors have been linked to TMZ resistance, role of solid tumor morphology microenvironment, particularly blood‐brain barrier (BBB), unknown. Here, authors investigate these using complex vitro model for GBM its surrounding BBB. The recapitulates...
Electrical stimulation (ES) of cells has been shown to induce a variety responses, such as cytoskeleton rearrangements, migration, proliferation, and differentiation. In this study, we have investigated whether monophasic biphasic pulsed ES could exert any effect on the proliferation differentiation human cardiac progenitor (hCPCs) isolated from heart fragments. Cells were cultured under continuous exposure or with fixed cycles for 1 3 days. Results indicate that neither protocol affected...
ABSTRACT Cardiac cell function is substantially influenced by the nature and intensity of mechanical loads cells experience. fibroblasts (CFs) are primarily involved in myocardial tissue remodeling: at onset specific pathological conditions, CFs activate, proliferate, differentiate, critically alter amount extra‐cellular matrix with important consequences for functioning. While cyclic strain has been shown to increase synthesis vitro, role cues proliferation unclear. We here developed a...
The extent of and the oncogenic role played by alternative splicing (AS) in cancer are well documented. Nonetheless, only few studies have attempted to dissect individual gene function at an isoform level. Here, we focus on AS factors during prostate progression, as these known undergo extensive potential affect hundreds downstream genes. We identified exon 7 (ex7) MBNL1 (Muscleblind-like 1) transcript being most differentially included cancer, both cell lines patients' samples. In contrast,...
Background and Aims HBV‐specific T‐cell receptor (HBV‐TCR) engineered T cells have the potential for treating HCC relapses after liver transplantation, but their efficacy can be hampered by concomitant immunosuppressive treatment required to prevent graft rejection. Our aim is molecularly engineer TCR‐T that could retain polyfunctionality in such patients while minimizing associated risk of organ Approach Results We first analyzed how drugs interfere with vivo function transplanted HBV‐HCC...
Background Chimeric antigen receptor (CAR) therapies have demonstrated potent efficacy in treating B-cell malignancies, but yet to meaningfully translate solid tumors. Nonetheless, they are of particular interest for the treatment glioblastoma, which is an aggressive form brain cancer with few effective therapeutic options, due their ability cross highly selective blood-brain barrier. Methods Here, we use our pooled screening platform, CARPOOL, expedite discovery CARs antitumor functions...
This communication describes a simple, rapid and cost effective method of embedding conductive flexible material within microfluidic devices as means to realize uniform electric fields cellular microenvironments. Fluidic channels electrodes are fabricated by traditional soft-lithography in conjunction with chemical etching PDMS. Devices can be deformable (thus allowing for combination electro-mechanical stimulation), they made from inexpensive materials easily assembled hand; this is thus...
Upon cardiac pathological conditions such as ischemia, microenvironmental changes instruct a series of cellular responses that trigger fibroblasts-mediated tissue adaptation and inflammation. A comprehensive model how early environmental may induce fibroblasts (CF) is far from being elucidated, partly due to the lack approaches involving complex simultaneous stimulation. Here, we provide first analysis human primary CF behavior by means multi-stimulus microdevice for combined application...
Abstract Engineered T cells transiently expressing tumor-targeting receptors are an attractive form of engineered cell therapy as they carry no risk insertional mutagenesis or long-term adverse side-effects. However, multiple rounds treatment often required, increasing patient discomfort and cost. To mitigate this, we sought to improve the antitumor activity transient by screening a panel small molecules targeting epigenetic regulators for their effect on cytotoxicity. Using model targetting...
ABSTRACT In tissue engineering, several factors play key roles in providing adequate stimuli for cells differentiation, particular biochemical and physical stimuli, which try to mimic the physiological microenvironments. Since electrical are important developing heart, we have developed an easy‐to‐use, cost‐effective cell culture platform, able provide controlled stimulation aimed at investigating influence of electric field stem differentiation process. This bioreactor consists stimulator...