A5072691908- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Genomic variations and chromosomal abnormalities
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Neuroendocrine regulation and behavior
- Nicotinic Acetylcholine Receptors Study
- Developmental Biology and Gene Regulation
- DNA and Nucleic Acid Chemistry
- Ubiquitin and proteasome pathways
- Invertebrate Immune Response Mechanisms
- Animal Genetics and Reproduction
- Adipose Tissue and Metabolism
- interferon and immune responses
- Muscle Physiology and Disorders
- Nuclear Structure and Function
- Physiological and biochemical adaptations
- Cancer-related gene regulation
- Animal Behavior and Reproduction
- Computational Drug Discovery Methods
- Plant Virus Research Studies
- Single-cell and spatial transcriptomics
Princeton University
2020-2024
Thomas Jefferson University
2024
University of Pennsylvania
2024
New York University
2024
University of Massachusetts Chan Medical School
2016-2020
Helmholtz Zentrum München
2018
Université de Strasbourg
2018
Inserm
2018
Laurentian University
2011-2014
Western University
2011
Offspring affected by sperm small RNAs Paternal dietary conditions in mammals influence the metabolic phenotypes of offspring. Although prior work suggests involvement epigenetic pathways, mechanisms remains unclear. Two studies now show that altered paternal diet affects level mouse sperm. Chen et al. injected transfer RNA (tRNA) fragments from males had been kept on a high-fat into normal oocytes. The progeny displayed disorders and concomitant alteration genes pathways. Sharma observed...
Past studies offer contradictory claims for the role of genome organization in regulation gene activity. Here, we show through high-resolution chromosome conformation analysis that
Abstract The relationship between chromatin organization and gene regulation remains unclear. While disruption of domains domain boundaries can lead to misexpression developmental genes, acute depletion regulators genome has a relatively small effect on expression. It is therefore uncertain whether expression state drive or changes in facilitate cell-type-specific activation Here, using the dorsoventral patterning Drosophila melanogaster embryo as model system, we provide evidence for...
Small RNAs derived from mature tRNAs, referred to as tRNA fragments or “tRFs,” are an emerging class of regulatory with poorly understood functions. We recently identified a role for one specific tRF—5′ tRF-Gly-GCC, tRF-GG—as repressor genes associated the endogenous retroelement MERVL, but mechanistic basis this regulation was unknown. Here, we show that tRF-GG plays in production wide variety noncoding RNAs—snoRNAs, scaRNAs, and snRNAs—that dependent on Cajal bodies stability activity....
RNA methyltransferase METTL6 is implicated in tumor cell growth and mouse energy consumption.
Two different models have been proposed to explain how the endpoints of chromatin looped domains (‘TADs’) in eukaryotic chromosomes are determined. In first, a cohesin complex extrudes loop until it encounters boundary element roadblock, generating stem-loop. this model, boundaries functionally autonomous: they an intrinsic ability halt movement incoming complexes that is independent properties neighboring boundaries. second, loops generated by boundary:boundary pairing. non-autonomous, and...
Paternal environmental conditions can influence phenotypes in future generations, but it is unclear whether offspring represent specific responses to particular aspects of the paternal exposure history, or a generic response ‘quality life’. Here, we establish effect model based on nicotine mice, enabling pharmacological interrogation specificity response. prior reproduction induced broad protective multiple xenobiotics male offspring. This manifested as increased survival following injection...
Though long non-coding RNAs (lncRNAs) represent a substantial fraction of the Pol II transcripts in multicellular animals, only few have known functions. Here we report that blocking activity Bithorax complex (BX-C) Fub-1 boundary is segmentally regulated by its own lncRNA. The located between Ultrabithorax (Ubx) gene and bxd/pbx regulatory domain, which responsible for regulating Ubx expression parasegment PS6/segment A1. consists two hypersensitive sites, HS1 HS2. an insulator while HS2...
Two different models have been proposed to explain how the endpoints of chromatin looped domains (“TADs”) in eukaryotic chromosomes are determined. In first, a cohesin complex extrudes loop until it encounters boundary element roadblock, generating stem-loop (and an unanchored loop). this model, boundaries functionally autonomous: they intrinsic ability halt movement incoming complexes that is independent properties neighboring boundaries. second, loops generated by boundary:boundary...
Two different models have been proposed to explain how the endpoints of chromatin looped domains (“TADs”) in eukaryotic chromosomes are determined. In first, a cohesin complex extrudes loop until it encounters boundary element roadblock, generating stem-loop. this model, boundaries functionally autonomous: they an intrinsic ability halt movement incoming complexes that is independent properties neighboring boundaries. second, loops generated by boundary:boundary pairing. non-autonomous, and...
Abstract Frost ( Fst ) is a gene associated with cold exposure in Drosophila melanogaster . We used real‐time PCR to assess whether induces expression of 10 different life stages D. , and adults seven other species. exposed groups individuals 0 °C (2 h), followed by 1 h recovery (22 °C). was significantly upregulated response eggs, third instar larvae, 2‐ 5‐day‐old male female Life which did not upregulate had high constitutive expression. located on the opposite strand an intron Diuretic...
Abstract Transvection, a chromosome pairing-dependent form of trans-based gene regulation, is potentially widespread in the Drosophila melanogaster genome and varies across cell types within tissues D. melanogaster, characteristics complex trait. Here, we demonstrate that trans-interactions at Malic enzyme (Men) locus are, fact, transvection as classically defined are plastic with respect to both genetic background environment. Using chromosomal inversions, show Men eliminated by changes...
(Abstracted from Nat Genet 2017;49:1502–1510) After fertilization, gametes undergo dramatic epigenomic reprogramming to initiate embryonic development. Retrotransposons are repetitive elements that form almost half of the mammalian genome; some transcribed after fertilization.