A5033138055- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- Adaptive optics and wavefront sensing
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Spectroscopy Techniques in Biomedical and Chemical Research
- Protein Degradation and Inhibitors
- Genetic Syndromes and Imprinting
- Astronomy and Astrophysical Research
- Posttraumatic Stress Disorder Research
- Skin Protection and Aging
- Nematode management and characterization studies
- RNA and protein synthesis mechanisms
- Frailty in Older Adults
- Cancer-related molecular mechanisms research
- Adolescent and Pediatric Healthcare
- Legume Nitrogen Fixing Symbiosis
- Histone Deacetylase Inhibitors Research
- Plant nutrient uptake and metabolism
- Education and Military Integration
- HIV/AIDS Research and Interventions
Ludwig-Maximilians-Universität München
2017-2022
Center for Integrated Protein Science Munich
2018-2020
Leibniz Institute for Astrophysics Potsdam
2001-2004
VA Hudson Valley Health Care System
2003
Covalent chemical modifications of cellular RNAs directly impact all biological processes. However, our mechanistic understanding the enzymes catalyzing these modifications, their substrates and functions, remains vague. Amongst RNA N 6 -methyladenosine (m A) is widespread found in messenger (mRNA), ribosomal (rRNA), noncoding RNAs. Here, we undertook a systematic screen to uncover new methyltransferases. We demonstrate that methyltransferase-like 5 (METTL5) protein catalyzes m A 18S rRNA at...
Bacterial accommodation inside living plant cells is restricted to the nitrogen-fixing root nodule symbiosis. In many legumes, bacterial uptake mediated via tubular structures called infection threads (ITs). To identify genes required for successful symbiotic infection, we screened an ethyl methanesulfonate mutagenized population of Lotus japonicus mutants defective in IT formation and cloned responsible gene, ERN1, encoding AP2/ERF transcription factor. We performed phenotypic analysis two...
RNA methyltransferase METTL6 is implicated in tumor cell growth and mouse energy consumption.
The RING E3 ubiquitin ligase UHRF1 controls DNA methylation through its ability to target the maintenance methyltransferase DNMT1 newly replicated chromatin. recruitment relies on ubiquitylation of histone H3 by UHRF1; however, how deposits onto is unknown. Here, we demonstrate that ubiquitin-like domain (UBL) essential for RING-mediated ubiquitylation. Using chemical crosslinking and mass spectrometry, biochemical assays, recombinant chromatin substrates, show UBL participates in structural...
Abstract Genome-wide DNA demethylation is a unique feature of mammalian development and naïve pluripotent stem cells. Here, we describe recently evolved pathway in which global hypomethylation achieved by the coupling active passive demethylation. TET activity required, albeit indirectly, for demethylation, mostly occurs at sites devoid binding. Instead, TET-mediated locus-specific necessary activating subset genes, including pluripotency germline marker Dppa3 ( Stella, Pgc7 ). DPPA3 turn...
Abstract STELLA is a robotic observatory with two fully automatic telescopes (STELLA‐I and STELLA‐II) located at the Teide Observatory in Tenerife, Spain. Not only are but also entire observatory, no human presence needed for observing ‐ not even remote control. STELLA‐I supports high‐resolution, fiber‐fed benchmounted echelle spectrograph wide‐field CCD imaging photometer while STELLA‐II feeds similar wide‐band testbed prototype adaptive optics telescopes. The first telescope scheduled...
DNA methylation (5-methylcytosine (5mC)) is critical for genome stability and transcriptional regulation in mammals. The discovery that ten-eleven translocation (TET) proteins catalyze the oxidation of 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxylcytosine (5caC) revolutionized our perspective on complexity modifications. However, what extent regulatory functions TET1 can be attributed its catalytic activity remains unclear. Here, we use engineering quantitative...
Abstract Cytosine DNA bases can be methylated by methyltransferases and subsequently oxidized TET proteins. The resulting 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxylcytosine (5caC) are considered demethylation intermediates as well stable epigenetic marks. To dissect the contributions of these cytosine modifying enzymes, we generated combinations Tet knockout (KO) embryonic stem cells (ESCs) systematically measured protein modification levels at transition from naive...
A Correction to this paper has been published: https://doi.org/10.1038/s41467-020-20453-0
Abstract Genome-wide DNA demethylation is a unique feature of mammalian development and naïve pluripotent stem cells. So far, it was unclear how mammals specifically achieve global hypomethylation, given the high conservation (de-)methylation machinery among vertebrates. We found that requires TET activity but mostly occurs at sites where proteins are not bound suggesting rather indirect mechanism. Among few specific genes activated by pluripotency germline marker Dppa3 ( Pgc7, Stella ),...
We present an overview and a brief report on the status of STELLA project (abbreviation for STELLar Activity). The STELLA-I telescope will be first robotic that feeds bench-mounted high-resolution Echelle spectrograph with set 50 100 μm fibres provides spectral resolutions up to 47,000 1 arcsec slit. is white-pupil design located in separated temperature-controlled room guarantee long-term stability. building have roll-off roof capable host two telescopes. First light planned summer 2002....
ABSTRACT The TET-oxidized cytosine derivatives, 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC), are considered DNA demethylation intermediates as well stable epigenetic marks in mammals. We compared modified enzyme levels TET-knockout cells during naive pluripotency exit found distinct differentiation-dependent contributions of TET1 TET2 to 5hmC 5fC formation. divergent argue for independent consecutive oxidation steps vivo with broad implications regulation.
Abstract Older traumatized veterans represent a population whose needs and problems pervade gerontological social work practice. They rarely identify themselves as nor do professionals often inquire about military service, especially if they are women. Social workers who see this can play critical role in helping examine traumatic experiences relate to range of presenting problems. Clinical issues pertaining engagement, assessment, interventions, transference termination with the aging...
Collagen‐degrading matrix metalloproteinases (MMPs) show increased activities predominantly in UV‐irradiated skin as well chronologically aged skin. MMPs are therefore reasoned to be associated with the degenerative alterations collagenous dermal extracellular matrix, which turn may accelerate signs of aging such wrinkle formation and sagging. In normal physiological processes MMP activity is controlled by endogenous tissue inhibitors (TIMPs). Here we report biotechnological production...
We present an overview and a brief report on the status of STELLA project (abbreviation for STELLar Activity). The STELLA-I telescope will be first robotic that feeds bench-mounted high-resolution Echelle spectrograph with set 50 100 μm fibres provides spectral resolutions up to 47,000 1 arcsec slit. is white-pupil design located in separated temperature-controlled room guarantee long-term stability. building have roll-off roof capable host two telescopes. First light planned summer 2002....