A5088664991- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Protein Degradation and Inhibitors
- Histone Deacetylase Inhibitors Research
- HIV Research and Treatment
- DNA Repair Mechanisms
- RNA Research and Splicing
- Thyroid Cancer Diagnosis and Treatment
- Gene expression and cancer classification
- Thyroid Disorders and Treatments
- Natural Language Processing Techniques
- Plant Molecular Biology Research
- Signaling Pathways in Disease
- Computational Drug Discovery Methods
- Genetics, Bioinformatics, and Biomedical Research
- Lipoproteins and Cardiovascular Health
- Glioma Diagnosis and Treatment
- Trypanosoma species research and implications
- Chromatin Remodeling and Cancer
- Cancer, Lipids, and Metabolism
- Ovarian cancer diagnosis and treatment
Helmholtz Zentrum München
2019-2024
University of Southern Denmark
2015-2023
University of Copenhagen
2017
Lomonosov Moscow State University
2015
Abstract DNA and histone modifications combine into characteristic patterns that demarcate functional regions of the genome 1,2 . While many ‘readers’ individual have been described 3–5 , how chromatin states comprising composite modification signatures, variants internucleosomal linker are interpreted is a major open question. Here we use multidimensional proteomics strategy to systematically examine interaction around 2,000 nuclear proteins with over 80 modified dinucleosomes representing...
Deposition of replication-independent histone variant H3.3 into chromatin is essential for many biological processes, including development and reproduction. Unlike replication-dependent H3.1/2 isoforms, expressed throughout the cell cycle becomes enriched in postmitotic cells with age. However, lifelong dynamics H3 replacement impact this process on organization remain largely undefined. Using quantitative middle-down proteomics we demonstrate that accumulates to near saturation levels...
Chromatin is dynamically reorganized when DNA replication forks are challenged. However, the process of epigenetic reorganization and its implication for fork stability poorly understood. Here we discover a checkpoint-regulated cascade chromatin signalling that activates histone methyltransferase EHMT2/G9a to catalyse heterochromatin assembly at stressed forks. Using biochemical single molecule fibre approaches, show G9a together with SUV39h1 induces compaction by accumulating repressive...
Post-translational modifications (PTMs) of histone proteins play a fundamental role in regulation DNA-templated processes. There is also growing evidence that proteolytic cleavage N-terminal tails, known as clipping, influences nucleosome dynamics and functional properties. Using top-down middle-down protein analysis by mass spectrometry, we report H2B H3 tail clipping human hepatocytes demonstrate relationship between co-existing PTMs H3. Histones undergo processing primary the...
DNA methylation (5-methylcytosine (5mC)) is critical for genome stability and transcriptional regulation in mammals. The discovery that ten-eleven translocation (TET) proteins catalyze the oxidation of 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxylcytosine (5caC) revolutionized our perspective on complexity modifications. However, what extent regulatory functions TET1 can be attributed its catalytic activity remains unclear. Here, we use engineering quantitative...
The heterodimeric histone chaperone FACT, consisting of SSRP1 and SPT16, contributes to dynamic nucleosome rearrangements during various DNA-dependent processes including transcription. In search post-translational modifications that may regulate the activity SPT16 were isolated from Arabidopsis cells analysed by mass spectrometry. Four acetylated lysine residues could be mapped within basic C-terminal region SSRP1, while three phosphorylated serine/threonine identified in acidic SPT16....
Trypanosomes diverged from the main eukaryotic lineage about 600 million years ago, and display some unusual genomic epigenetic properties that provide valuable insight into early processes employed by ancestors to regulate chromatin-mediated functions. We analysed Trypanosoma brucei core histones high mass accuracy middle-down spectrometry map histone post-translational modifications (PTMs) elucidate cis-histone combinatorial PTMs (cPTMs). T. are heavily modified intricate cPTMs patterns,...
The mitotic deacetylase complex MiDAC has recently been shown to play a vital physiological role in embryonic development and neurite outgrowth. However, how functionally intersects with other chromatin-modifying regulators is poorly understood. Here, we describe physical interaction between the histone H3K27 demethylase UTX, complex-specific subunit of enhancer-associated MLL3/4 complexes, MiDAC. We demonstrate that UTX bridges association complexes by interacting ELMSAN1, scaffolding Our...
Current methods of preoperative diagnostics frequently fail to discriminate between benign and malignant thyroid neoplasms. In encapsulated follicular-patterned tumors (EnFPT), this discrimination is challenging even using histopathological analysis. Autoantibody response against tumor-associated antigens a well-documented phenomenon with prominent diagnostic potential; however, autoantigenicity remains poorly explored.Objectives were exploration antigen repertoire identification candidate...
The chromatin environment at origins of replication is thought to influence DNA initiation in eukaryotic genomes. However, it remains unclear how and which features control the firing early-efficient (EE) or late-inefficient (LI) origins. Here, we use site-specific recombination single-locus isolation purify EE LI Saccharomyces cerevisiae. Using mass spectrometry, define protein composition native regions surrounding start sites. In addition known origin interactors, find microtubule-binding...
Abstract The mitotic deacetylase complex MiDAC has recently been shown to play a vital physiological role in embryonic development and neurite outgrowth. However, how functionally intersects with other chromatin-modifying regulators is poorly understood. Here, we describe physical interaction between the histone H3K27 demethylase UTX, complex-specific subunit of enhancer-associated MLL3/4 complexes, MiDAC. We demonstrate that UTX bridges association complexes by interacting ELMSAN1,...