A5026940015- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Light effects on plants
- Algal biology and biofuel production
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Plant Genetic and Mutation Studies
- Plant Molecular Biology Research
- Microbial Metabolic Engineering and Bioproduction
- ATP Synthase and ATPases Research
- Lipid metabolism and biosynthesis
- Plant tissue culture and regeneration
- Plant Micronutrient Interactions and Effects
- Genetically Modified Organisms Research
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Plant responses to water stress
- Plant-Microbe Interactions and Immunity
- Protein Structure and Dynamics
- Crop Yield and Soil Fertility
- Genetic and Environmental Crop Studies
- Electrocatalysts for Energy Conversion
- Electron Spin Resonance Studies
- Cassava research and cyanide
University of Copenhagen
2013-2023
Ludwig-Maximilians-Universität München
2008-2020
Frederiksberg Hospital
2016
Phosphorus (P) is an essential macronutrient, and P deficiency limits plant productivity. Recent work showed that affects electron transport to photosystem I (PSI), but the underlying mechanisms are unknown. Here, we present a comprehensive biological model describing how disrupts photosynthetic machinery chain through series of sequential events in barley (Hordeum vulgare). reduces orthophosphate concentration chloroplast stroma levels inhibit ATP synthase activity. Consequently, protons...
Short-term changes in illumination elicit alterations thylakoid protein phosphorylation and reorganization of the photosynthetic machinery. Phosphorylation LHCII, light-harvesting complex photosystem II, facilitates its relocation to I permits excitation energy redistribution between photosystems (state transitions). The kinase STN7 is required for LHCII state transitions flowering plant Arabidopsis thaliana. reversible, but extensive efforts identify phosphatase(s) that dephosphorylate have...
Chloroplasts of land plants characteristically contain grana, cylindrical stacks thylakoid membranes. A granum consists a core appressed membranes, two stroma-exposed end and margins, which connect pairs grana membranes at their lumenal sides. Multiple forces contribute to stacking, but it is not known how the extreme curvature margins generated maintained. We report identification CURVATURE THYLAKOID1 (CURT1) protein family, conserved in cyanobacteria. The four Arabidopsis thaliana CURT1...
Abstract Flowering plants control energy allocation to their photosystems in response light quality changes. This includes the phosphorylation and migration of light-harvesting complex II (LHCII) proteins (state transitions or short-term response) as well long-term alterations thylakoid composition (long-term LTR). Both responses require protein kinase STN7. Here, we show that signaling pathways triggering state LTR diverge at, immediately downstream from, STN7 activity can be regulated...
Developmental or metabolic changes in chloroplasts can have profound effects on the rest of plant cell. Such intracellular responses are associated with signals that originate and convey information their physiological status to nucleus, which leads large-scale gene expression (retrograde signaling). A screen designed identify components retrograde signaling resulted discovery so-called genomes uncoupled (gun) mutants. Genetic evidence suggests chloroplast protein GUN1 integrates derived...
The oxygen-evolving complex of eukaryotic photosystem II (PSII) consists four extrinsic subunits, PsbO (33 kDa), PsbP (23 PsbQ (17 kDa) and PsbR (10 encoded by seven nuclear genes, PsbO1 (At5g66570), PsbO2 (At3g50820), PsbP1 (At1g06680), PsbP2 (At2g30790), PsbQ1 (At4g21280), PsbQ2 (At4g05180) (At1g79040). Using Arabidopsis insertion mutant lines, we show that PsbP1, but not PsbP2, is essential for photoautotrophic growth, whereas plants lacking both forms and/or normal growth rates. Complete...
In photosynthetic electron transport, large multiprotein complexes are connected by small diffusible carriers, the mobility of which is challenged macromolecular crowding. For thylakoid membranes higher plants, a long-standing question has been two mobile plastoquinone or plastocyanin, mediates transport from stacked grana thylakoids where photosystem II (PSII) localized to distant unstacked regions that harbor PSI. Here, we confirm plastocyanin long-range carrier employing mutants with...
A lack of individual plastid ribosomal proteins (PRPs) can have diverse phenotypic effects in Arabidopsis thaliana, ranging from embryo lethality to compromised vitality, with the latter being associated photosynthetic lesions and decreases expression proteins. In this study, reverse genetics was employed study function eight PRPs, five which (PRPS1, -S20, -L27, -L28 -L35) not been functionally characterised before. case PRPS17, only leaky alleles or RNA interference lines had analysed...
Plant chloroplasts are light-driven cell factories that have great potential to act as a chassis for metabolic engineering applications. Using plant chloroplasts, we demonstrate how photosynthetic reducing power can drive pathway synthesise bio-active natural product. For this purpose, stably engineered the dhurrin from Sorghum bicolor into of Nicotiana tabacum (tobacco). Dhurrin is cyanogenic glucoside and its synthesis amino acid tyrosine catalysed by two membrane-bound cytochrome P450...
Thylakoid phosphorylation is predominantly mediated by the protein kinases STN7 and STN8. While primarily catalyzes LHCII phosphorylation, which enables to migrate from photosystem (PS) II PSI, STN8 mainly phosphorylates PSII core proteins. The reversible of proteins thought regulate repair cycle supercomplex stability, play a role in modulating folding thylakoid membranes. Earlier studies clearly demonstrated considerable substrate overlap between two STN kinases, raising possibility...
The thylakoid membrane system of higher plant chloroplasts consists interconnected subdomains appressed and nonappressed bilayers, known as grana stroma lamellae, respectively. CURVATURE THYLAKOID1 (CURT1) protein complexes mediate the shape stacks in a dosage-dependent manner facilitate curvature at margins, interface between lamellae. Although are highly conserved among land plants, functional relevance stacking remains unclear. Here, we show that inhibiting CURT1-mediated alteration...
Thylakoid membranes in land plant chloroplasts are organized into appressed and nonappressed membranes, which contribute to the control of energy distribution between two photosystems (PSI PSII) from associated light-harvesting complexes (LHCs). Under fluctuating light conditions, fast reversible phosphorylation N-terminal thylakoid protein domains changes electrostatic forces induce modifications organization. To gain insight role dynamics phosphorylation, we used targeted proteomics...
Abstract Plant scientists and farmers are facing major challenges in providing food nutritional security for a growing population, while preserving natural resources biodiversity. Moreover, this should be done adapting agriculture to climate change by reducing its carbon footprint. To address these challenges, there is an urgent need breed crops that more resilient suboptimal environments. Huge progress has recently been made understanding the physiological, genetic molecular bases of plant...
Abstract To meet the increasing global demand for food, feed, fibre and other plant‐derived products, a steep increase in crop productivity is scientifically technically challenging imperative. The CropBooster‐P project, response to H2020 call ‘Future proofing our plants’, developing roadmap plant research improve crops critical future of European agriculture by yield, nutritional quality, value non‐food applications sustainability. However, if we want efficiently production Europe...
Protein phosphorylation is a major mode of regulation metabolism, gene expression and cell architecture. In chloroplasts, reversible proteins known to regulate number prominent processes, for instance photosynthesis, starch metabolism. The complements the involved chloroplast protein kinases (cpPKs) phosphatases (cpPPs) are largely unknown, except 6 (4 cpPKs 2 cpPPs) which have been experimentally identified so far. We employed combinations programs predicting N-terminal transit peptides...