A5007124296- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- Plant Micronutrient Interactions and Effects
- Potato Plant Research
- Plant-Microbe Interactions and Immunity
- Plant and fungal interactions
- Mycorrhizal Fungi and Plant Interactions
- Plant Parasitism and Resistance
- Plant responses to elevated CO2
- Plant Virus Research Studies
- Seed Germination and Physiology
- Soil Carbon and Nitrogen Dynamics
- Polymer-Based Agricultural Enhancements
- Insect-Plant Interactions and Control
- Agriculture, Plant Science, Crop Management
- Aluminum toxicity and tolerance in plants and animals
- Plant Gene Expression Analysis
- Microbial Metabolites in Food Biotechnology
- Plant Physiology and Cultivation Studies
- Bioeconomy and Sustainability Development
- Metabolism, Diabetes, and Cancer
- Botany and Plant Ecology Studies
Institut Jean-Pierre Bourgin
2015-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
AgroParisTech
2012-2025
Université Paris-Saclay
2017-2025
RIKEN Center for Sustainable Resource Science
2022
Centre National de la Recherche Scientifique
2011-2020
Institut National de la Recherche Agronomique
2004-2012
Département Santé des Plantes et Environnement
2003-2008
Lerøy (Norway)
2007
Stavros Niarchos Foundation
2007
Summary These experiments were carried out to investigate whether accumulation of carbohydrate leads decreased expression genes involved in photosynthesis. Addition glucose autotrophic cell suspension cultures Chenopodium led a large and reversible decrease the steady state transcript levels rbcS, cab atp‐& within 5 h, but did not 18S rRNA or for two glycolytic enzymes. Run‐on transcription isolated nuclei showed that rate had been decreased. [ 35 S]Methionine feeding de novo synthesis...
Nitrate is an essential nutrient, and involved in many adaptive responses of plants, such as localized proliferation roots, flowering or stomatal movements. How nitrate-specific mechanisms are regulated at the molecular level poorly understood. Although Arabidopsis ANR1 transcription factor appears to control stimulation lateral root elongation response nitrate, no regulators nitrate assimilation have so far been identified higher plants. Legume-specific symbiotic nitrogen fixation under...
Abstract Nitrogen (N) is an essential macronutrient for plants. N levels in soil vary widely, and plants have developed strategies to cope with deficiency. However, the regulation of these adaptive responses coordinating signals that underlie them are still poorly understood. The aim this study was characterize starvation adult Arabidopsis (Arabidopsis thaliana) a spatiotemporal manner by integrative, multilevel global approach analyzing growth, metabolites, enzyme activities, transcript...
Abstract Dynamic reprogramming of gene regulatory networks (GRNs) enables organisms to rapidly respond environmental perturbation. However, the underlying transient interactions between transcription factors (TFs) and genome-wide targets typically elude biochemical detection. Here, we capture both stable TF-target within minutes after controlled TF nuclear import using time-series chromatin immunoprecipitation (ChIP-seq) and/or DNA adenine methyltransferase identification (DamID-seq). The...
Abstract Nitrate promotes seed germination at low concentrations in many plant species, and functions as both a nutrient signal. As nutrient, it is assimilated via nitrite to ammonium, which then incorporated into amino acids. reductase (NR) catalyses the reduction of nitrate nitrite, committed step assimilation. Seed sensitivity affected by other environmental factors, such light after-ripening, genotypes. Mode action has been well documented Arabidopsis thaliana hedge mustard Sisymbrium...
Abiotic and biotic factors cause plant wounding trigger complex short- long-term responses at the local systemic levels. These are under control of signaling pathways, which still poorly understood. Here, we show that rapid activation clade-A mitogen-activated protein kinases (MAPKs) MPK3 MPK6 by depends on upstream MAPK MKK4 MKK5 but is independent jasmonic acid (JA) signaling. In addition, this fast module does not wound-triggered JA accumulation in Arabidopsis (Arabidopsis thaliana),...
Nitrate signaling improves plant growth under limited nitrate availability and, hence, optimal resource use for crop production. Whereas several transcriptional regulators of have been identified, including the Arabidopsis thaliana transcription factor NIN-LIKE PROTEIN7 (NLP7), additional are expected to fine-tune this pivotal physiological response. Here, we characterized NLP2 as a top-tier regulator early response gene regulatory network. interacts with NLP7 in vivo and shares key...
Summary Expression of the gene Nrt2Np , which encodes a putative high‐affinity nitrate transporter Nicotiana plumbaginifolia was studied under variable physiological conditions. is rapidly induced by very low concentrations and repressed reduced nitrogen metabolites. Furthermore, expressed in coordination with other genes involved assimilation ( Nia, Nii ). A deficiency reductase activity, accompanied high internal concentration levels metabolites, e.g. glutamine, leads to an overexpression...
Abstract The influence of elevated [CO 2 ] on the uptake and assimilation nitrate ammonium was investigated by growing tobacco plants in hydroponic culture with m or 1 ambient 800 p.p.m. ]. Leaves roots were harvested at several times during diurnal cycle to investigate levels transcripts for a high‐affinity transporter ( NRT2 ), reductase NIA cytosolic plastidic glutamine synthetase GLN1 , GLN2 activity synthetase, rate 15 N‐nitrate N‐ammonium uptake, nitrate, ammonium, amino acids,...
Tobacco seedlings were grown in nutrient agar at a range of ammonium nitrate concentrations either without added sucrose, or with 100 mol m –3 sucrose. In the absence nitrogen‐limited plants had increased levels glucose, fructose and decreased chlorophyll, protein, Rubisco activity, but level transcript for small subunit ( Rbc S) did not decrease compared nitrogen‐sufficient plants. When sucrose was to seedlings, there an increase glucose leaves, growth increased, chlorophyll protein...
The high affinity nitrate transport system in Arabidopsis thaliana involves one gene and potentially seven genes from the NRT1 NRT2 family, respectively. Among them, NRT2.1, NRT2.2, NRT2.4 NRT2.7 proteins have been shown to are localized on plasmalemma or tonoplast membranes. NRT2.2 play a role uptake soil solution by root cells while is responsible for loading seed vacuole. We undertaken functional characterization of third member NRT2.6 gene. was weakly expressed most plant organs its...
Optimal timing of flowering, a major determinant for crop productivity, is controlled by environmental and endogenous cues. Nutrients are known to modify flowering time; however, our understanding how nutrients interact with the pathways, especially at shoot apical meristem (SAM), still incomplete. Given negative side-effects nitrogen fertilization, it essential understand its mode action sustainable production. We investigated moderate restriction nitrate integrated into network SAM, which...
Abstract NLP7 (NIN-LIKE-PROTEIN 7) is the major transcriptional factor responsible for primary nitrate response (PNR), but role of its homolog, NLP6, in nitrogen signaling and interplay between NLP6 remain to be elucidated. In this study, we show that, like NLP7, nuclear localization via a retention mechanism dependent, nucleocytosolic shuttling both independent each other. Compared with single mutants, nlp6nlp7 double mutant displays synergistic growth retardation phenotype nitrate. The...
Summary Tobacco transformants that express an antisense RBCS construct were used to investigate the consequences of a lesion in photosynthetic carbon metabolism for nitrogen and secondary metabolism. The results show inhibition photosynthesis decrease sugar levels leads general metabolism, dramatic changes metabolites. response was particularly clear plants received excess nitrogen. In these conditions, Rubisco activity led nitrate reductase activity, accumulation nitrate, amino acid larger...
In a low-input agricultural context, plants facing temporal nutrient deficiencies need to be efficient. By comparing the effects of NO(3)(-)-starvation in two lines Arabidopsis thaliana (RIL282 and 432 from Bay-0xShahdara population), this study aimed screen physiological mechanisms allowing one genotype withstand NO(3)(-)-deprivation better than another rate relative importance processes such as nitrate uptake, storage, recycling. These lines, chosen because their contrasted shoot N...