A5048914421- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant pathogens and resistance mechanisms
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Plant Parasitism and Resistance
- Transgenic Plants and Applications
- Plant Micronutrient Interactions and Effects
- CRISPR and Genetic Engineering
- Plant Pathogens and Fungal Diseases
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Microbial bioremediation and biosurfactants
- Lipid metabolism and biosynthesis
- Banana Cultivation and Research
- Biological and pharmacological studies of plants
- Pesticide and Herbicide Environmental Studies
- Evolution and Genetic Dynamics
- Coastal wetland ecosystem dynamics
Centre National de la Recherche Scientifique
2011-2024
Université de Toulouse
2016-2024
Laboratoire des Interactions Plantes Micro-Organismes
1998-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2008-2024
Institut National Polytechnique de Toulouse
2017-2022
Université Toulouse III - Paul Sabatier
2021-2022
Université Paris-Saclay
2017-2020
Institut des Sciences des Plantes de Paris Saclay
2016-2020
Université Paris Cité
2017-2020
Institut Jean-Pierre Bourgin
2000-2017
Abstract Nitrogen (N) is an essential macronutrient for plants. N levels in soil vary widely, and plants have developed strategies to cope with deficiency. However, the regulation of these adaptive responses coordinating signals that underlie them are still poorly understood. The aim this study was characterize starvation adult Arabidopsis (Arabidopsis thaliana) a spatiotemporal manner by integrative, multilevel global approach analyzing growth, metabolites, enzyme activities, transcript...
After transcription, mRNA editing in angiosperm chloroplasts and mitochondria results the conversion of cytidine to uridine by deamination. Analysis Arabidopsis thaliana mutants affected RNA have shown that many pentatricopeptide repeat proteins (PPRs) are required for specific deamination events. PPR been be sequence-specific binding allowing recognition C edited. The C-terminal DYW domain present factors has proposed catalyze deamination, as it shows sequence similarities with deaminases...
RNA editing is converting hundreds of cytosines into uridines during organelle gene expression land plants. The pentatricopeptide repeat (PPR) proteins are at the core this posttranscriptional modification. Even if a PPR protein defines site, DYW domain same or another believed to catalyze deamination. To give insight editosome, we performed tandem affinity purification plastidial CHLOROPLAST BIOGENESIS 19 (CLB19) factor. Two proteins, dually targeted mitochondria and chloroplasts, were...
The root cap has a fundamental role in sensing environmental cues as well regulating growth via altered meristem activity. Despite this well-established the control of developmental processes roots, cap's function nutrition remains obscure. Here, we uncover its phosphate by targeted cellular inactivation or transport complementation Arabidopsis, using transactivation strategy with an innovative high-resolution real-time (33)P imaging technique. Remarkably, diminutive size cells at...
Four hundred and fifty-eight genes coding for PentatricoPeptide Repeat (PPR) proteins are annotated in the Arabidopsis thaliana genome. Over past 10 years, numerous reports have shown that many of these function organelles to target specific transcripts involved post-transcriptional regulation. Therefore, they thought be important players coordination between nuclear organelle genome expression. Only four been described addressed outside organelles, indicating some PPRs could regulations...
Hydathodes are water pores found on leaves of a wide range vascular plants and the sites guttation. We report here detailed anatomy cauliflower (Brassicaoleracea) Arabidopsis (Arabidopsis thaliana) hydathodes. Hydathode surface presents resembling stomata giving access to large cavities. Beneath, epithem is composed lacunar highly vascularized parenchyma offering direct connection between leaf xylem vessels. hydathode were responsive ABA light similar stomata. The flg22 flagellin peptide,...
To understand how the identity of an organ can be switched, we studied transformation lateral root primordia (LRP) into shoot meristems in Arabidopsis segments. In this system, cytokinin-induced conversion does not involve formation callus-like structures. Detailed analysis showed that sequence starts with a mitotic pause and is concomitant differential expression regulators development. The requires presence apical stem cells, only LRP at stages VI or VII switched. It engaged as soon cell...
Summary Uridine nucleotides can be formed by energy‐consuming de novo synthesis or the energy‐saving recycling of nucleobases resulting from nucleotide catabolism. Uracil phosphoribosyltransferases (UPRTs; EC 2.4.2.9) are involved in salvage pyrimidines catalyzing formation uridine monophosphate (UMP) uracil and phosphoribosylpyrophosphate. To date, UPRTs described as non‐essential, enzymes. In present work, six genes annotated Arabidopsis genome examined through phylogenetic functional...
In the context of climate warming, plants will be facing an increased risk epidemics as well emergence new highly aggressive pathogen species. Although a permanent increase temperature strongly affects plant immunity, underlying molecular mechanisms involved are still poorly characterized. this study, we aimed to uncover genetic bases resistance remaining efficient at elevated Ralstonia solanacearum species complex (RSSC), one most harmful phytobacteria causing bacterial wilt. To start...
Quantitative disease resistance (QDR) remains the most prevalent form of plant in crop fields and wild habitats. Genome-wide association studies (GWAS) have proved to be successful deciphering quantitative genetic basis complex traits such as QDR. To unravel genetics QDR devastating worldwide bacterial pathogen Ralstonia solanacearum, we performed a GWAS by challenging highly polymorphic local mapping population Arabidopsis thaliana with four R. solanacearum type III effector (T3E) mutants,...
Abstract Plant immunity is often negatively impacted by heat stress. However, the underlying molecular mechanisms remain poorly characterized. Based on a genome‐wide association mapping approach, this study aims to identify in Arabidopsis thaliana genetic bases of robust resistance devastating pathogen Ralstonia solanacearum under A local population was phenotyped against R. GMI1000 strain at 27 and 30 °C. To obtain precise description architecture natural variation quantitative disease...
C to U editing is one of the post-transcriptional steps which are required for proper expression chloroplast and mitochondrial genes in plants. It depends on several proteins acting together include PLS-class pentatricopeptide repeat (PPR). DYW2 was recently shown be many sites both organelles. In particular almost all associated with E+ subfamily PPR depending DYW2, suggesting that function E+-type proteins. Here we strengthened this link by identifying 16 major controlled 3 proteins:...
Xanthomonas campestris pv. (Xcc) is a seed-transmitted vascular pathogen causing black rot disease on cultivated and wild Brassicaceae. Xcc enters the plant tissues preferentially via hydathodes, which are organs localized at leaf margins. To decipher both physiological virulence strategies deployed by during early stages of infection, transcriptomic profile was analysed 3 days after entry into cauliflower hydathodes. Despite absence visible tissue alterations despite biotrophic lifestyle,...
Temperature elevation drastically affects plant defense responses to
Abstract Ralstonia solanacearum gram‐negative phytopathogenic bacterium exerts its virulence through a type III secretion system (T3SS) that translocates effectors (T3Es) directly into the host cells. T3E is finely controlled at posttranslational level by helper proteins, T3SS control and chaperones. The HpaP protein, one of substrate specificity switch (T3S4) was previously highlighted as factor on Arabidopsis thaliana Col‐0 accession. In this study, we set up genome‐wide association...