Electrical stimulation (ES) of cells has been shown to induce a variety responses, such as cytoskeleton rearrangements, migration, proliferation, and differentiation. In this study, we have investigated whether monophasic biphasic pulsed ES could exert any effect on the proliferation differentiation human cardiac progenitor (hCPCs) isolated from heart fragments. Cells were cultured under continuous exposure or with fixed cycles for 1 3 days. Results indicate that neither protocol affected...

New biomimetic magnetite nanoparticles (hereafter BMNPs) with sizes larger than most common superparamagnetic were produced in the presence of recombinant MamC protein from Magnetococcus marinus MC-1 and functionalized doxorubicin (DOXO) intended as potential drug nanocarriers. Unlike inorganic nanoparticles, BMNPs controls their size morphology, providing them magnetic properties consistent a large moment per particle; moreover, it provides novel surface properties. display isoelectric...

Biomimetic magnetic nanoparticles mediated by magnetosome proteins (BMNPs) are potential innovative tools for cancer therapy since, besides being multifunctional platforms, they can be manipulated an external gradient field (GMF) and/or alternating (AMF), mediating targeting and hyperthermia, respectively. We evaluated the cytocompatibility/cytotoxicity of BMNPs Doxorubicin (DOXO)-BMNPs in presence/absence GMF 4T1 MCF-7 cells as well their cellular uptake. analyzed biocompatibility vivo...

Nanomedicine covers the application of nanotechnologies in medicine. Of particular interest is setup highly-cytocompatible nanoparticles for use as drug carriers and/or medical imaging. In this context, luminescent are appealing nanodevices with great potential imaging tumor or other targetable cells, and several strategies under investigation. Biomimetic apatite represent candidates choice nanomedicine due to their high intrinsic biocompatibility highly accommodative properties structure,...

We have developed a straightforward, one-pot, low-temperature hydrothermal method to transform oyster shell waste particles (bCCP) from the species Crassostrea gigas (Mg-calcite, 5 wt% Mg) into hydroxyapatite (HA) micro/nanoparticles. The influence of P reagents (H3PO4, KH2PO4, and K2HPO4), P/bCCP molar ratios (0.24, 0.6, 0.96), digestion temperatures (25-200 °C), times (1 week-2 months) on transformation process was thoroughly investigated. At 1 week, minimum temperature yield full...

Bioinspired citrate–carbonate–apatite (cAp) nanocrystals doped with divalent transition metal (M) ions, i.e., Mn2+, Co2+, and Ni2+, were prepared by batch thermal decomplexing of Ca2+/M/citrate/phosphate/carbonate solutions at 80 37 °C, initial Ca2+/M molar ratios 9:1 5:5, different crystallization times ranging from 1 to 96 h. A thorough chemical, crystallographic, morphological characterization was carried out on the revealing that (i) using similar conditions amount incorporated M...

Tumor-targeted drug-loaded nanocarriers represent innovative and attractive tools for cancer therapy. Several magnetic nanoparticles (MNPs) were analyzed as potential tumor-targeted after functionalization with anti-Met oncogene (anti-Met/HGFR) monoclonal antibody (mAb) doxorubicin (DOXO). Their cytocompatibility, stability, immunocompetence (immunoprecipitation), their interactions cells in vitro (Perl’s staining, confocal microscopy, cytotoxic assays: MTT, real time toxicity) tumors vivo...

This research explores the transformation of biogenic CaCO3 microparticles (Ø <45 µm) from oyster shell waste into either luminescent Eu- or Tb-doped calcium phosphate (apatites), using a sustainable “one-step” and...

Abstract Biogenic CaCO 3 microparticles obtained from oyster shells Crassostrea gigas were used as starting material for synthesizing Co 2+ , Mg and Mn -doped apatite nano-submicroparticles, through a one-step hydrothermal conversion. The conversion was completed at 200 °C 7 days, yielding metal-doped whitlockite in percentages of 5.3 wt% when adding 28.7 0 . Samples cytocompatible with murine pancreatic endothelial cells (MS1), mesenchymal stem (m17.ASC), osteoblast’s progenitors (mOBPs)...

A major obstacle to an effective myocardium stem cell therapy has always been the delivery and survival of implanted cells in heart. Better engraftment can be achieved if are administered as aggregates, which maintain their extra-cellular matrix (ECM). We have generated spheroid aggregates less than 24 h by seeding human cardiac progenitor (hCPCs) onto methylcellulose hydrogel-coated microwells. Cells within spheroids maintained expression stemness/mesenchymal ECM markers, growth factors...

Multifunctional biomimetic nanoparticles (NPs) are acquiring increasing interest as carriers in medicine and basic research since they can efficiently combine labels for subsequent tracking, moieties specific cell targeting, bioactive molecules, e.g., drugs. In particular, because of their easy synthesis, low cost, good biocompatibility, high resorbability, surface functionalization, pH-dependent solubility, nanocrystalline apatites promising candidates nanocarriers. This work describes the...

Abstract Novel MamC‐mediated biomimetic magnetic nanoparticles (BMNPs) are proposed as valuable carriers for targeted chemotherapy because of the size (36 ± 12 nm) and surface properties conferred by MamC coating. They super‐paramagnetic at room body temperatures, have a large moment per particle, mediate hyperthermia, cytocompatible, and, having negative charge physiological pH, can be efficiently coupled with DOXOrubicin (DOXO) monoclonal antibody (mAb) directed against human...

New therapeutic strategies are required in cancer therapy. Considering the prominent role of tumor-associated macrophages (TAMs) development and progression cancer, re-education TAMs tumor microenvironment (TME) could represent a potential approach for immunotherapy. display an irregular unfolded protein response (UPR) their endoplasmic reticulum (ER) to endure environmental stress ensure anti-cancer immunity. Therefore, nanotechnology be attractive tool modulate UPR TAMs, providing...

The design of novel nanomaterials that can be used as multifunctional platforms allowing the combination therapies is gaining increased interest. Moreover, if this nanomaterial intended for a targeted drug delivery, use several guidance methods to increase efficiency also crucial. Magnetic nanoparticles (MNPs) allow and strategies. In fact, MNPs simultaneously nanocarriers magnetic hyperthermia agents and, moreover, they guided toward target by an external field their functionalization with...

Skeletal muscles represent 40% of body mass and its native regenerative capacity can be permanently lost after a traumatic injury, congenital diseases, or tumor ablation. The absence physiological regeneration hinder muscle repair preventing normal tissue functions. To date, engineering (TE) represents one promising option for treating injuries wasting. In particular, hydrogels derived from the decellularized extracellular matrix (dECM) are widely investigated in applications thanks to their...

Superparamagnetic iron oxide nanoparticles (MNPs) have recently been investigated for biological applications with promising results, owing to their ability be targeted and heated by magnetic fields. Silica is a very suitable coating material MNPs, facilitating the loading of targeting moieties drug delivery. However, potential toxicity SiO2-coated MNPs remains major concern clinical application. The synthesis, via wet-chemistry, physico-chemical characterization Fe3O4 silica coated...

In the field of Nanomedicine, there is an increasing demand for new inorganic nanophosphors with low cytotoxicity and efficient loading-release ability drugs applications in bioimaging drug delivery. This work assesses potentiality matured Eu-doped citrate-coated carbonated apatite nanoparticles to be used as theranostic platforms, bioimaging, luminescent nanoprobes, delivery applications, using Doxorubicin a model drug. The adsorption isotherm fits Langmuir–Freundlich (LF) model, showing...

Synthetic nanostructured hybrid composites based on collagen and nanocrystalline apatites are interesting materials for the generation of scaffolds bone tissue engineering. In this work, mineralized fibrils were prepared in presence citrate Eu3+. Citrate is an indispensable essential structural/functional component bone. Eu3+ endows necessary luminescent features to be potentially employed as a diagnostic tool biomedical applications. The assembly mineralization performed by neutralization...

The eggshell is a biomineral consisting of CaCO3 in the form calcite phase and pervading organic matrix (1-3.5 wt.%). Transforming particles into calcium phosphate (apatite) micro-nanoparticles opens door to repurposing waste as materials with potential biomedical applications, fulfilling principles circular economy. Previous methods obtain these consisted mainly two steps, first one involving calcination eggshell. In this research, direct transformation by one-pot hydrothermal method...

The nucleation of apatite nanoparticles on exfoliated graphene nanoflakes has been successfully carried out by the sitting drop vapor diffusion method, with aim producing cytocompatible hybrid nanocomposites both components. flakes were prepared sonication-assisted, liquid-phase exfoliation technique, using following biomolecules as dispersing surfactants: lysozyme, L-tryptophan, N-acetyl-D-glucosamine, and chitosan. Results from mineralogical, spectroscopic, microscopic characterization...

The transplantation of mesenchymal stem cells (MSCs) is a promising approach for tissue repairing, but the survival, engraftment and migration these in recipient may be compromised by necrotic oxidative microenvironment present there.Herein we investigated whether cardiac progenitor (CPCs) isolated from human heart biopsies could protected an stress treatment with clovamide, natural polyphenolic compound previously Trifolium pratense (red clover) cocoa (Theobroma cacao) endowed anti-oxidant...