A5083770622- Nanoparticle-Based Drug Delivery
- Characterization and Applications of Magnetic Nanoparticles
- Geomagnetism and Paleomagnetism Studies
- CRISPR and Genetic Engineering
- Nanoplatforms for cancer theranostics
- RNA and protein synthesis mechanisms
- Magnetic and Electromagnetic Effects
- Biofuel production and bioconversion
- Bacteriophages and microbial interactions
- Enzyme Catalysis and Immobilization
- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- Animal Nutrition and Physiology
- Moringa oleifera research and applications
- Antimicrobial Peptides and Activities
- Enzyme Production and Characterization
- RNA regulation and disease
- Plant Micronutrient Interactions and Effects
- Ultrasound and Hyperthermia Applications
- Enzyme Structure and Function
- Inhalation and Respiratory Drug Delivery
- Electrochemical Analysis and Applications
- Iron oxide chemistry and applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Minerals Flotation and Separation Techniques
CIC bioGUNE
2023-2025
Universidad de Granada
2018-2024
CIC nanoGUNE
2022-2023
Purpose: The lack of specificity conventional chemotherapy is one the main difficulties to be solved in cancer therapy. Biomimetic magnetoliposomes are successful controlled-release systems, hyperthermia, and active targeting agents by functionalization their surface with monoclonal antibodies. membrane receptor Leucine-rich repeat-containing G-protein coupled 5 (LGR5) stands out as colorectal (CRC) biomarker appears related treatment resistance development metastasis. aim this study was...
New biomimetic magnetite nanoparticles (hereafter BMNPs) with sizes larger than most common superparamagnetic were produced in the presence of recombinant MamC protein from Magnetococcus marinus MC-1 and functionalized doxorubicin (DOXO) intended as potential drug nanocarriers. Unlike inorganic nanoparticles, BMNPs controls their size morphology, providing them magnetic properties consistent a large moment per particle; moreover, it provides novel surface properties. display isoelectric...
Immobilization of enzymes has been extensively required in a wide variety industrial applications as way to ensure functionality and the potential enzyme recycling after use. In particular, immobilization on magnetic nanoparticles (MNPs) could offer reusability by means recovery concentration, along with increased stability robust activity under different physicochemical conditions. present work, microbial α-amylase (AmyKS) xylanase (XAn11) were both immobilized types MNPs [MamC-mediated...
Conventional chemotherapy against colorectal cancer (CRC), the third most common in world, includes oxaliplatin (Oxa) which induces serious unwanted side effects that limit efficiency of treatment. Therefore, alternative therapeutic approaches are urgently required. In this work, biomimetic magnetic nanoparticles (BMNPs) mediated by MamC were coupled to Oxa evaluate potential Oxa-BMNP nanoassembly for directed local delivery drug as a proof concept future development targeted CRC....
In this work we report on the synthesis and characterization of magnetic nanoparticles two distinct origins, one inorganic (MNPs) other biomimetic (BMNPs), latter based a process bacterial synthesis. Each these kinds particles has its own advantages when used separately with biomedical purposes. Thus, BMNPs present an isoelectric point below neutrality (around pH 4.4), while MNPs show zero-zeta potential at 7, appear to be excellent agents for hyperthermia. This means that are better suited...
Current chemotherapy for colorectal cancer (CRC) includes the use of oxaliplatin (Oxa), a first-line cytotoxic drug which, in combination with irinotecan/5-fluorouracil or biologic agents, increases survival rate patients. However, administration this induces side effects that limit its application patients, making it necessary to develop new tools targeted chemotherapy. MamC-mediated biomimetic magnetic nanoparticles coupled Oxa (Oxa-BMNPs) have been previously demonstrated efficiently...
Choline kinase α1 (ChoKα1) has become an excellent antitumor target. Among all the inhibitors synthetized, new compound Ff35 shows capacity to inhibit ChoKα1 activity. However, soluble is also capable of inhibiting choline uptake, making inhibitor not selective for ChoKα1. In this study, we designed a protocol with aim disentangling whether biological action due inhibition enzyme and/or uptake. Moreover, offer alternative avoid uptake caused by Ff35, since coupling novel biomimetic magnetic...
The production of Reactive Oxygen Species after exposure HepG2 cells to alternating magnetic fields can explain the loss cell viability in spite a negligible increase temperature.
Magnetococcus marinus magnetosome-associated protein MamC, expressed as recombinant, has been proven to mediate the formation of novel biomimetic magnetic nanoparticles (BMNPs) that are successful drug nanocarriers for targeted chemotherapy and hyperthermia agents. These BMNPs present several advantages over inorganic nanoparticles, such larger sizes allow former have moment per particle, an isoelectric point at acidic pH values, which allows both stable functionalization physiological value...
MamC-mediated biomimetic magnetic nanoparticles (BMNPs) have emerged as one of the most promising nanomaterials due to their features (superparamagnetic character and large moment per particle), novel surface properties determined by MamC, biocompatibility ability hyperthermia agents. However, current clinical application is limited fact that, in order be able reach an effective temperature at target site, relatively high nanoparticle concentration, well field strength and/or AC frequency...
Abstract The role of magnetosome associated proteins on the in vitro synthesis magnetite nanoparticles has gained interest, both to obtain a better understanding biomineralization process and be able produce novel magnetosome-like biomimetic nanoparticles. Up now, only one recombinant protein been used at time form precipitates, being that scenario far enough from what probably occurs magnetosome. In present study, Mms6 MamC Magnetococcus marinus MC-1 have magnetites. Our results show...
The importance of egg natural defences to prevent bacterial contamination and their relation with hen age in extended production cycles were evaluated. Egg-white from eggs different groups (up 100-weeks-old) lines (Hy-Line white brown) inoculated Gram-positive Staphylococcus aureus or Gram-negative Salmonella Typhimurium, ranging 103-106 CFU/mL. Our results show that concentrations egg-white lysozyme and, particularly, ovotransferrin are important modulate survival a dose-dependent matter....
The design of novel nanomaterials that can be used as multifunctional platforms allowing the combination therapies is gaining increased interest. Moreover, if this nanomaterial intended for a targeted drug delivery, use several guidance methods to increase efficiency also crucial. Magnetic nanoparticles (MNPs) allow and strategies. In fact, MNPs simultaneously nanocarriers magnetic hyperthermia agents and, moreover, they guided toward target by an external field their functionalization with...
In the field of Nanomedicine, there is an increasing demand for new inorganic nanophosphors with low cytotoxicity and efficient loading-release ability drugs applications in bioimaging drug delivery. This work assesses potentiality matured Eu-doped citrate-coated carbonated apatite nanoparticles to be used as theranostic platforms, bioimaging, luminescent nanoprobes, delivery applications, using Doxorubicin a model drug. The adsorption isotherm fits Langmuir–Freundlich (LF) model, showing...
Abstract Numerous enzymes have the potential to upgrade biomass, converting it into high-tech materials for new applications. However, features of natural often limit their use beyond chemical conversion substrate. The development strategies enzymatic biomass high-value may broaden range applications and enzyme design techniques. A relevant case is lytic polysaccharide monooxygenase (LPMO), a class that catalyzes oxidative cleavage glycosidic bonds. Here, we show an ancestral LPMO can...
Magnetite nanoparticles in the magnetosomes formed by magnetotactic bacteria present unique magnetic properties that make them ideal nanoparticle with potential use several biotechnological applications. These magnetoliposomes are organelles crystals of magnetite (Fe3O4) or greigite (Fe3S4) surrounded a lipid bilayer. However, scaling up production these cannot be achieved currently because slow growth and strict physiological characteristics bacteria. An alternative to solve this problem is...
MamC, an integral protein of the magnetosome membrane, has recently been proposed as a strong candidate to produce biomimetic (magnetosome-like) magnetite nanoparticles that could be used alternative magnetosomes in different applications such nanocarriers. The secondary structure contains two helical transmembrane domains connected by α-helical loop oriented toward lumen. In this loop, residues Glu66 and Asp70 seem responsible for template effect controls nucleation and/or growth vitro....
Magnetite nanoparticles (MNPs) are being used in a number of nanotechnological applications, especially biomedical, both diagnosis and therapeutics such as hyperthermia agents drug nanocarriers for targeted chemotherapy. However, the development efficient methodologies to produce novel MNPs with specific requirements needed biomedical applications is still challenging. In this context, biomimetic approaches taking use magnetosome proteins expressed recombinant and/or polyamino acids becoming...
The synergy between directed chemotherapy and thermal therapy (both magnetic hyperthermia photothermia) mediated by a nanoassembly composed of functionalized biomimetic nanoparticles (BMNPs) with the chemotherapeutic drug doxorubicin (DOXO) covered polymer poly(lactic-co-glycolic acid) (PLGA), decorated TAT peptide (here referred to as TAT–PLGA(DOXO-BMNPs)) is explored in present study. rationale behind this lies an optimization nanoformulation DOXO-BMNPs, already demonstrated be more...
Among the strategies employed to overcome development of multidrug-resistant bacteria, directed chemotherapy combined with local therapies (e.g., magnetic hyperthermia) has gained great interest. A nano-assembly coupling antimicrobial peptide AS-48 biomimetic nanoparticles (AS-48-BMNPs) was demonstrated have potent bactericidal effects on both Gram-positive and Gram-negative bacteria when activity hyperthermia. Nevertheless, intracellular pathogens remain challenging due difficulty drug...