A5053941815- Skin and Cellular Biology Research
- Wound Healing and Treatments
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Cellular Mechanics and Interactions
- Lymphatic System and Diseases
- Dermatologic Treatments and Research
- Dermatological and Skeletal Disorders
- Autoimmune Bullous Skin Diseases
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Periodontal Regeneration and Treatments
- 3D Printing in Biomedical Research
- Dermatology and Skin Diseases
- Immunotherapy and Immune Responses
- Electrospun Nanofibers in Biomedical Applications
- Axon Guidance and Neuronal Signaling
- Wnt/β-catenin signaling in development and cancer
- Ubiquitin and proteasome pathways
- Nerve injury and regeneration
- MicroRNA in disease regulation
- Bone Tissue Engineering Materials
- RNA Interference and Gene Delivery
- Thyroid Disorders and Treatments
- Fibroblast Growth Factor Research
Istituti di Ricovero e Cura a Carattere Scientifico
2010-2024
Explora (Italy)
2020-2024
Istituto Dermopatico dell'Immacolata
2006-2020
Institute of Cell Biology and Neurobiology
2000-2019
Institute of Molecular and Cell Biology
2017
Zero to Three
2003
Sapienza University of Rome
2000
Th subsets are defined according to their production of lineage-indicating cytokines and functions. In this study, we have identified a subset human cells that infiltrates the epidermis in individuals with inflammatory skin disorders is characterized by secretion IL-22 TNF-alpha, but not IFN-gamma, IL-4, or IL-17. analogy Th17 subset, cytokine profile been named Th22 subset. clones derived from patients psoriasis were stable culture exhibited transcriptome clearly separate those Th1, Th2,...
Placenta growth factor (PlGF) is a member of the vascular endothelial (VEGF) family, comprising at least five cytokines specifically involved in regulation and/or lymphatic endothelium differentiation. Several lines evidence indicate role for PlGF monocyte chemotaxis and potentiating activity VEGF, but exact function this cytokine not fully understood. To define biological vivo, we have produced transgenic mouse model overexpressing skin by using keratin 14 promoter cassette. Our data that...
Abstract Adipose tissue–derived stem cells ( ASC s) are gaining increasing consideration in tissue repair therapeutic application. Recent evidence indicates that s enhance skin animal models of impaired wound healing. To assess the activity autologous vs. allogeneic treatment diabetic ulcers, we functionally characterized and investigated their potential to promote healing with respect nondiabetic ones. from streptozotocin‐induced type 1 mice were analyzed either freshly isolated as stromal...
IL-22 has a pathogenetic role in psoriasis, where it is responsible for the altered proliferation and differentiation of keratinocytes induces inflammatory molecules. The IL-22-induced effects are mediated by STAT3, whose activity proportional to acetylation lysine (Lys)685 phosphorylation tyrosine (Tyr)705. Lys 685 STAT3 inhibited sirtuin (SIRT)1, class III deacetylase promoting keratinocyte differentiation. Due opposite SIRT1, we investigated whether could be regulated SIRT1 through...
Recessive dystrophic epidermolysis bullosa (RDEB) is a genodermatosis characterized by fragile skin forming blisters that heal invariably with scars. It due to mutations in the COL7A1 gene encoding type VII collagen, major component of anchoring fibrils connecting cutaneous basement membrane dermis. Identical often result inter- and intra-familial disease variability, suggesting additional modifiers contribute RDEB course. Here, we studied monozygotic twin pair presenting markedly different...
Keratinocyte proliferation and migration are crucial steps for the rapid closure of epidermis during wound healing, but molecular mechanisms involved in this cellular response remain to be completely elucidated. Here, by situ hybridization we characterize expression pattern miR-203 after induction mouse epidermis, showing that its is downregulated highly proliferating keratinocytes 'migrating tongue', whereas it strongly expressed differentiating cells skin outside wound. Furthermore,...
Recessive dystrophic epidermolysis bullosa (RDEB) is a blistering disease caused by mutations in the gene encoding type VII collagen (C7). RDEB associated with fibrosis, which responsible for severe complications. The phenotypic variability observed siblings suggests that epigenetic modifications contribute to severity. Identifying changes may help uncover molecular mechanisms underlying pathogenesis and new therapeutic targets.
Abstract BACKGROUND Melanoma metastasizes by different mechanisms comprising direct invasion of the surrounding tissue and spreading via lymphatic or vascular system. Despite their clinical relevance, molecular that guide route localization metastases in tissues are not well known. Recent studies tumor types have shown endothelial growth factor‐C (VEGF‐C), which displays a high specificity for endothelium, is involved tumor‐induced lymphangiogenesis metastatic spread. The authors studied...
Journal Article Granulocyte/macrophage colony‐stimulating factor treatment of human chronic ulcers promotes angiogenesis associated with de novo vascular endothelial growth transcription in the ulcer bed Get access F. Cianfarani, Cianfarani Laboratory Molecular and Cell Biology, VIII Dermatology Division Search for other works by this author on: Oxford Academic Google Scholar R. Tommasi, Tommasi C.M. Failla, Failla M.T. Viviano, Viviano G. Annessi, Annessi Histopathology Department, Istituto...
Epidermal wound repair is a complex process involving the fine orchestrated regulation of crucial cell functions, such as proliferation, adhesion and migration. Using an in vitro model that recapitulates central aspects epidermal healing, we demonstrate transcription factor HIF1 strongly stimulated keratinocyte cultures submitted to mechanical injury. Signals generated by scratch wounding stabilise HIF1α protein, which requires activation PI3K pathway independently oxygen availability. We...
Abstract Medical devices, such as silicone‐based prostheses designed for soft tissue implantation, often induce a suboptimal foreign‐body response which results in hardened avascular fibrotic capsule around the device, leading to patient discomfort or implant failure. Here, it is proposed that additive manufacturing techniques can be used deposit durable coatings with multiscale porosity on surfaces promote optimal integration. Specifically, “liquid rope coil effect”, exploited via direct...
Recessive dystrophic epidermolysis bullosa (RDEB) is a skin fragility disorder caused by mutations in the COL7A1 gene encoding type VII collagen, cutaneous basement membrane component essential for epidermal–dermal adhesion. Hallmarks of disease are unremitting blistering and chronic wounds with severe inflammation fibrosis. MicroRNAs (miRNAs) post‐transcriptional regulators expression also implicated fibrotic processes. However, role miRNAs RDEB fibrosis almost unexplored. Our study aimed...
Abstract Background Vascular endothelial growth factor‐C ( VEGF ‐C), a lymphatic vessel factor, has been involved in the formation of lymph nodal metastases different tumor types. Early evidences indicate that ‐C expression human primary melanoma could be predictive metastases, whereas role lymphangiogenesis is still controversial. Methods By immunohistochemical analysis, we investigated or CC chemokine receptor 7 expression, together with and blood network, 36 patients skin melanomas at...
Abstract Regenerative medicine approaches, specifically stem cell technologies, have demonstrated significant potential to treat a diverse array of pathologies. However, such approaches resulted in modest clinical benefit, which may be attributed poor retention/survival at the disease site. A delivery system that facilitates regional and repeated target tissues can provide enhanced efficacy therapies when localized high doses cells is required. In this study, new regenerative reservoir...