A5006311064- RNA Research and Splicing
- Cancer-related Molecular Pathways
- Genomics and Chromatin Dynamics
- PI3K/AKT/mTOR signaling in cancer
- Hippo pathway signaling and YAP/TAZ
- Circular RNAs in diseases
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- MicroRNA in disease regulation
- Chromosomal and Genetic Variations
- SARS-CoV-2 and COVID-19 Research
- Genomic variations and chromosomal abnormalities
- Cynara cardunculus studies
- Health Systems, Economic Evaluations, Quality of Life
- Bioactive Natural Diterpenoids Research
- Mast cells and histamine
- Cancer Research and Treatments
- Phytochemical Studies and Bioactivities
- Effects and risks of endocrine disrupting chemicals
- Wnt/β-catenin signaling in development and cancer
- Medical and Biological Ozone Research
- HIV/AIDS Research and Interventions
- Virology and Viral Diseases
Ospedale Antonio Cardarelli
2021
National Cancer Institute
2017
McGill University
2000-2014
McGill University Health Centre
2011
Harvard University
2004
University of Naples Federico II
1999
Circular RNAs are a class of endogenous with various functions in eukaryotic cells. Worthy note, circular play critical role cancer. Currently, nothing is known about their head and neck squamous cell carcinoma (HNSCC). The identification HNSCC might become useful for diagnostic therapeutic strategies HNSCC. Using samples from 115 patients, we find that circPVT1 over-expressed tumors compared to matched non-tumoral tissues, particular enrichment patients TP53 mutations. up- down-regulation...
4E-transporter (4E-T) is one of several proteins that bind the mRNA 5'cap-binding protein, eukaryotic initiation factor 4E (eIF4E), through a conserved binding motif. We previously showed 4E-T nucleocytoplasmic shuttling which mediates import eIF4E into nucleus. At steady state, predominantly cytoplasmic and concentrated in bodies conspicuously resemble recently described processing (P-bodies), are believed to be sites decay. In this paper, we demonstrate colocalizes with decapping factors...
In eukaryotes, a surveillance mechanism known as nonsense-mediated decay (NMD) degrades the mRNA when premature-termination codon (PTC) is present. NMD requires translation to read frame of and detect PTC. During pre-mRNA splicing, exon–exon junction complex (EJC) recruited region 20–24 nt upstream exon on mature mRNA. The presence PTC from EJC elicits NMD. Eukaryotic initiation factor 4A (eIF4A) III nuclear protein that interacts physically or functionally with factors eIF4G eIF4B,...
Abstract Background Long-range interactions between regulatory DNA elements such as enhancers, insulators and promoters play an important role in regulating transcription. As chromatin contacts have been found throughout the human genome different cell types, spatial transcriptional control is now viewed a general mechanism of gene expression regulation. Chromosome Conformation Capture Carbon Copy (5C) its variant Hi-C are techniques used to measure interaction frequency (IF) specific...
Spatial chromatin organization is emerging as an important mechanism to regulate the expression of genes. However, very little known about genome architecture at high-resolution in vivo . Here, we mapped three-dimensional human Hox clusters with chromosome conformation capture (3C) technology. We show that computational modeling 3C data sets can identify candidate regulatory proteins and gene expression. genes encode evolutionarily conserved master regulators development which strict control...
Eukaryotic initiation factor (eIF) 4E, the mRNA 5′-cap-binding protein, mediates association of eIF4F with 5′-cap structure to stimulate cap-dependent translation in cytoplasm. The assembly eIF4E into complex is negatively regulated through a family repressor proteins, called eIF4E-binding proteins (4E-BPs). also present nucleus, where it thought nuclear-cytoplasmic transport certain mRNAs. transported nucleus via its interaction 4E-T (4E-transporter), but unclear how retained nucleus. Here...
Abstract One of the major genomics challenges is to better understand how correct gene expression orchestrated. Recent studies have shown spatial chromatin organization critical in regulation expression. Here, we developed a suite computer programs identify conformation signatures with 5C technology http://Dostielab.biochem.mcgill.ca . We identified dynamic HoxA cluster associated cellular differentiation. Genome-wide signature identification might uniquely disease-associated states and...
Abstract Metformin is a commonly prescribed type II diabetes medication that exhibits promising anticancer effects. Recently, these effects were found to be associated, at least in part, with modulation of microRNA expression. However, the mechanisms by which single modulated microRNAs mediate metformin are not entirely clear and knowledge such process could vital maximize potential therapeutic benefits this safe well-tolerated therapy. Our analysis here revealed expression miR-21-5p was...
The coronavirus disease 2019 (COVID-19) pandemic caused by the emergent severe acute respiratory syndrome 2 (SARS-CoV-2) threatens global public health, and there is an urgent need to develop safe effective vaccines. Here, we report generation preclinical evaluation of a novel replication-defective gorilla adenovirus-vectored vaccine encoding pre-fusion stabilized Spike (S) protein SARS-CoV-2. We show that our candidate, GRAd-COV2, highly immunogenic both in mice macaques, eliciting...
We have previously shown that melatonin exerts tumor suppressor activities by inducing the p38-p53 axis. This occurred within a few hours while no data are available on how pathway can be sustained long term. Here we show miR-24, which has been demonstrated to target genes involved in DNA repair process, targets p38, p53, PML and H2AX simultaneously. long-term treatment with decrease miR-24 levels post-transcriptionally, pairs long-wave regulation of cell proliferation, damage, RNA...
// Claudio Pulito 1 , Federica Mori Andrea Sacconi 2 Luca Casadei 3 Maria Ferraiuolo Cristina Valerio Raffaela Santoro Frauke Goeman Anna Maidecchi 4 Luisa Mattoli Cesare Manetti Silvia Di Agostino Paola Muti 5 Giovanni Blandino 2, Sabrina Strano 1, Molecular Chemoprevention Unit, Medicine Area, Regina Elena National Cancer Institute, Rome, Italy Translational Oncogenomic Department of Chemistry, University Rome "La Sapienza", Aboca SpA Società Agricola, Sansepolcro, Oncology, Juravinski...
Gene therapy of Usher syndrome type 1B (USH1B) due to mutations in the large Myosin VIIA (MYO7A) gene is limited by packaging capacity adeno-associated viral (AAV) vectors. To overcome this, we have previously developed dual AAV8 vectors which encode human MYO7A (dual AAV8.MYO7A). Here show that subretinal administration 1.37E+9 1.37E+10 genome copies a good-manufacturing-practice-like lot AAV8.MYO7A improves retinal defects mouse model USH1B. The same was used non-human primates at doses...
Although genetic or epigenetic alterations have been shown to affect the three-dimensional organization of genomes, utility chromatin conformation in classification human disease has never addressed.Here, we explore whether can be used classify leukemia. We map HOXA gene cluster a panel cell lines with 5C chromosome capture technology, and use data train test support vector machine classifier named 3D-SP. show that 3D-SP is able accurately distinguish leukemias expressing MLL-fusion proteins...
Osteosarcoma (OS) is the most aggressive type of primary solid tumor that develops in bone. Whilst conventional chemotherapy can improve survival rates, outcome for patients with metastatic or recurrent OS remains poor, so novel treatment agents and strategies are required. Research into new anticancer therapies has paved way utilisation natural compounds as they typically less expensive toxic compared to chemotherapeutics. Previously published works indicate Agave exhibits properties,...
Over the past decade, newly designed cancer therapies have not significantly improved survival of patients diagnosed with Malignant Pleural Mesothelioma (MPM). Among a limited number genes that are frequently mutated in MPM several them encode proteins belong to HIPPO tumor suppressor pathway. The anticancer effects top flower standardized extract Filipendula vulgaris (Dropwort) were characterized "in vitro" and vivo" models MPM. At molecular level, two "omic" approaches used investigate...
ABSTRACT The COVID-19 pandemic caused by the emergent SARS-CoV-2 coronavirus threatens global public health and there is an urgent need to develop safe effective vaccines. Here we report generation preclinical evaluation of a novel replication-defective gorilla adenovirus-vectored vaccine encoding pre-fusion stabilized Spike (S) protein SARS-CoV2. We show that our candidate, GRAd- COV2, highly immunogenic both in mice macaques, eliciting functional antibodies which neutralize infection block...