A5045593444- Hepatitis B Virus Studies
- Hepatitis C virus research
- SARS-CoV-2 and COVID-19 Research
- Liver Disease Diagnosis and Treatment
- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- Vibrio bacteria research studies
- Viral Infections and Outbreaks Research
- T-cell and B-cell Immunology
- Pneumonia and Respiratory Infections
- Immunodeficiency and Autoimmune Disorders
- COVID-19 diagnosis using AI
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Streptococcal Infections and Treatments
- HIV/AIDS Research and Interventions
- HIV/AIDS drug development and treatment
- Virology and Viral Diseases
- Cytomegalovirus and herpesvirus research
- Tuberculosis Research and Epidemiology
- COVID-19 epidemiological studies
- Diphtheria, Corynebacterium, and Tetanus
- Biosensors and Analytical Detection
Bambino Gesù Children's Hospital
2020-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2021-2025
Centre International De Reference Chantal Biya
2023
University of Milan
2019-2021
European Institute of Oncology
2021
Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda
2021
University of Rome Tor Vergata
2014-2020
Abstract From February to April 2020, Lombardy (Italy) reported the highest numbers of SARS-CoV-2 cases worldwide. By analyzing 346 whole genomes, we demonstrate presence seven viral lineages in Lombardy, frequently sustained by local transmission chains and at least two likely have originated Italy. Six single nucleotide polymorphisms (five them non-synonymous) characterized sequences, none affecting N-glycosylation sites. The lineages, clusters within three them, revealed that community...
Breakthrough SARS-CoV-2 infections in fully vaccinated individuals are considered a consequence of waning immunity. Serum antibodies represent the most measurable outcome vaccine-induced B cell memory. When decline, memory cells expected to persist and perform their function, preventing clinical disease. We investigated whether BNT162b2 mRNA vaccine induces durable functional vivo against 3, 6, 9 months after second dose cohort health care workers (HCWs). While we observed physiological...
Hepatitis B virus (HBV) reactivation during immunosuppression can lead to severe acute hepatitis, fulminant liver failure, and death. Here, we investigated hepatitis surface antigen (HBsAg) genetic features underlying this phenomenon by analyzing 93 patients: 29 developing HBV 64 consecutive patients with chronic infection (as control). HBsAg diversity was analyzed population-based ultradeep sequencing (UDS). Before reactivation, 51.7% of were isolated core antibody (anti-HBc) positive,...
Since SARS-CoV-2-based disease (COVID-19) spreads as a pandemic, the necessity of highly sensitive molecular diagnosis that can drastically reduce false negatives reverse transcription PCR (rtPCR) results, raises major clinical need. Here we evaluated performance ddPCR-based assay to quantify SARS-CoV-2 titer in 55 suspected COVID-19 cases with negative rtPCR results thanks in-house ddPCR (targeting RdRp and host RNaseP). Samples were collected at ASST-GOM Niguarda between February May 2020...
Mass SARS-Cov-2 vaccination campaign represents the only strategy to defeat global pandemic we are facing. Immunocompromised patients represent a vulnerable population at high risk of developing severe COVID-19 and thus should be prioritized in programs study vaccine efficacy. Nevertheless, most data on efficacy safety available vaccines derive from trials conducted healthy individuals; hence, studies immunogenicity SARS-CoV2 such populations deeply needed. Here, perform an observational...
Specific memory B cells and antibodies are a reliable read-out of vaccine efficacy. We analysed these biomarkers after one two doses BNT162b2 vaccine. The second dose significantly increases the level highly specific antibodies. Two months dose, antibody levels decline, but continue to increase, thus predicting sustained protection from COVID-19. show that although mucosal IgA is not induced by vaccination, migrate in response inflammation secrete at sites. first may lead an insufficient...
Objective The involvement of HBV DNA integration in promoting hepatocarcinogenesis and the extent to which intrahepatic reservoir modulates liver disease progression remains poorly understood. We examined reservoir, occurrence its impact on hepatocyte transcriptome hepatitis B ‘e’ antigen (HBeAg)-negative chronic (CHB). Design Liver tissue from 84 HBeAg-negative patients with CHB low (n=12), moderate (n=25) high (n=47) serum was analysed. Covalently closed circular (cccDNA), pregenomic RNA...
Pertussis continues to be a highly contagious respiratory infection, especially in children, with cyclical peaks of disease spread every three five years. Here, we report relevant cases B. pertussis infection between August 2023 and January 2024, compare them prevalence pediatric patients admitted the Reference Italian Pediatric Hospital, located Rome, from 2015 July 2023. A total 5464 tests for were performed during study period, 6.9% positive. At time COVID-19 pandemic, there was sharp...
HBsAg immune-escape mutations can favor HBV-transmission also in vaccinated individuals, promote immunosuppression-driven HBV-reactivation, and increase fitness of drug-resistant strains. Stop-codons enhance HBV oncogenic-properties. Furthermore, as a consequence the overlapping structure genome, some or stop-codons derive from drug-resistance RT. This study is aimed at gaining insight prevalence characteristics immune-associated escape mutations, chronically HBV-infected patients...
Abstract Background Recent studies showed that plasma SARS-CoV-2 RNA seems to be associated with worse COVID-19 outcome. However, whether specific population can at higher risk of viremia are date unexplored. Methods This cross-sectional proof-of-concept study included 41 SARS-CoV-2-positive adult individuals (six affected by haematological malignancies) hospitalized two major hospital in Milan, for those demographic, clinical and laboratory data were available. load was quantified ddPCR...
Since the start of SARS-CoV-2 pandemic, children aged ≤ 12 years have always been defined as underrepresented in terms infections' frequency and severity. By correlating transmission dynamics with clinical virological features 612 positive patients years, we demonstrated a sizeable circulation different lineages over four pandemic waves paediatric population, sustained by local chains. Age < 5 highest viral load, gamma delta clades positively influence this transmission. No correlations...
Abstract Background In recent months, Bordetella pertussis has reappeared after maintaining a low rate for many years. Although is usually characterized by favorable course, several factors can contribute to the severity of disease, such as mixed respiratory infections. this study, we evaluate B.pertussis cases observed in pediatric population followed at Bambino Gesù Children's Hospital and analyzed potential impact co-infections relation disease severity. Methods From January May 2024,...
We aimed to assess the utilization of Genotype Resistance Testing (GRT) by Infectious Diseases Units across Italy. A cross-sectional study was conducted involving a questionnaire distributed Unit in web-based survey using Google Forms software utilized and spread via email or cellphone. Responses were obtained from 101 Units. Among these centers, only seven (6.9%) reported not performing GRT at any time. Of 94 centers GRT, 52 (55.3%) sent blood samples external laboratories. Notably, 6/35...
Viral co-infections are frequently observed among children, but whether specific viral interactions enhance or diminish the severity of respiratory disease is still controversial. This study aimed to investigate type mono- and by also evaluating correlations in 3525 samples from pediatric in/outpatients screened Allplex Respiratory Panel Assays with a Severe Acute Syndrome-COronaVirus 2 (SARS-CoV-2) test available. Overall, were detected 37.8% patients more specimens children lower tract...
Immunization of vulnerable populations with distinct immunity often results in suboptimal immunogenicity, durability, and efficacy.
Patients affected by Inflammatory Bowel Disease (IBD) present higher risk for infection and suboptimal response upon vaccination. The immunogenicity of SARS-CoV2 vaccination is still largely unknown in adolescents or young adults IBD (pIBD). We investigated the safety BNT162B2 mRNA COVID-19 vaccine 27 pIBD, as compared to 30 healthy controls (HC). Immunogenicity was measured anti-SARS-CoV2 IgG (anti-S anti-trim Ab) before vaccination, after 21 days (T21) 7 second dose (T28). profile close...
Owing to an increasing number of infections in adults, Lactococcus (L.) garvieae has gained recognition as emerging human pathogen, causing bacteraemia and septicaemia. In September 2020, four paediatric onco-hematologic patients received a platelet concentrate from the same adult donor at Bambino Gesù Children's Hospital IRCCS, Rome. Three experienced L. sepsis one day after transfusion. The pediatric isolates donor's concentrates were retrospectively collected for whole-genome sequencing...
Accurate measurement of the hepatitis B virus (HBV) DNA is important for management patients with chronic HBV infection. Here, performance Xpert® Viral Load test (Xpert Load) versus Roche COBAS® Ampliprep/COBAS® TaqMan® system (CAP/CTM HBV) v2.0 was evaluated. From September 2017 to December 2017, a total 876 prospectively collected or archived serum EDTA plasma specimens from subjects chronically infected were tested using Xpert and CAP/CTM assays. Of tested, 560 within quantitative range...
We aimed to evaluate the safety and immunogenicity of BNT162b2 vaccine in young people with Down syndrome (DS), compare their humoral immune response those healthy controls (HC). Individuals DS HC received vaccine. Longitudinal blood samples were collected on day vaccination, twenty-one days after first dose, seven second six months dose. Both local systemic adverse events reported by participants mild. Pain at injection site was most event, while fever event. Humoral responses showed a...
To complement RT-qPCR testing for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, many countries have introduced the use rapid antigen tests. As they generally display lower real-life performances than expected, their correct positioning as frontline screening is still controversial. Despite lack data from daily clinical use, third generation microfluidic assays (such LumiraDx SARS-CoV-2 Ag test) recently been suggested to similar and proposed...