Janine Michel
A5066932819- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- COVID-19 diagnosis using AI
- Poxvirus research and outbreaks
- Biosensors and Analytical Detection
- Herpesvirus Infections and Treatments
- Bacillus and Francisella bacterial research
- COVID-19 Clinical Research Studies
- COVID-19 epidemiological studies
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Respiratory viral infections research
- Bacteriophages and microbial interactions
- Vaccine Coverage and Hesitancy
- Plant Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- Influenza Virus Research Studies
- Vector-Borne Animal Diseases
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Disaster Response and Management
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Hepatitis B Virus Studies
- Advanced Chemical Sensor Technologies
Robert Koch Institute
2015-2025
European Molecular Biology Laboratory
2016-2019
Bernhard Nocht Institute for Tropical Medicine
2015-2016
Wellington Hospital
2012
University Hospital of Geneva
1992
Analysis of 179 new Ebola virus sequences from patient samples collected in Guinea between March 2014 and January 2015 shows how different lineages evolved spread West Africa. Miles Carroll colleagues report describe the genetic evolution circulating Africa, based on 2015. Their analysis Africa Sierra Leone, Liberia. is currently witnessing most extensive (EBOV) outbreak so far recorded1,2,3. Until now, there have been 27,013 reported cases 11,134 deaths. The origin thought to a zoonotic...
SARS-CoV-2 is the causative of COVID-19 disease, which has spread pandemically around globe within a few months. It therefore necessary to collect fundamental information about its epidemiology and treatment, as well virus itself. While been identified rapidly, detailed ultrastructural analysis cell biology architecture still in infancy. We studied morphology morphometry comparison SARS-CoV it appears Vero cultures by using conventional thin section electron microscopy tomography. Both...
Repeated vaccinations and infections have led to diverse states of hybrid immunity against SARS-CoV-2 in the global population. However, age comorbidities can compromise protection severe disease, antibody-mediated is undercut by viral immune escape mutations. Whether what extent durable T cell responses compensate for reduced humoral immunity, particularly elderly, not been investigated. Here, we utilize SARS-CoV-2-specific pan-coronavirus-derived peptide pools, including or excluding spike...
By January, 2016, all known transmission chains of the Ebola virus disease (EVD) outbreak in west Africa had been stopped. However, there is concern about persistence reproductive tract men who have survived EVD. We aimed to use biostatistical modelling describe dynamics RNA load seminal fluid, including clearance parameters.In this longitudinal study, we recruited discharged from three treatment units Guinea between January and July, 2015. Participants provided samples fluid at follow-up...
Abstract We detected delayed and reduced antibody T-cell responses after BNT162b2 vaccination in 71 elderly persons (median age 81 years) compared with 123 healthcare workers 34 Germany. These data emphasize that nonpharmaceutical interventions for coronavirus disease remain crucial additional immunizations the might become necessary.
SARS-CoV-2, the cause of COVID-19, requires reliable diagnostic methods to track circulation this virus. Following development RT-qPCR meet need in January 2020, it became clear from interlaboratory studies that reported Ct values obtained for different laboratories showed high variability. Despite were explored as a quantitative cut off aid clinical decisions based on viral load. Consequently, there was introduce standards support estimation SARS-CoV-2 load specimens. In collaborative...
Three months after a coronavirus disease (COVID-19) outbreak in Kupferzell, Germany, population-based study (n = 2,203) found no RT-PCR-positives. IgG-ELISA seropositivity with positive virus neutralisation tests was 7.7% (95% confidence interval (CI): 6.5–9.1) and 4.3% negative tests. We estimate 12.0% CI: 10.4–14.0%) infected adults (24.5% asymptomatic), six times more than notified. Full hotspot containment confirms the effectiveness of prompt protection measures. However, 88% naïve are...
The reliable detection of SARS-CoV-2 has become one the most important contributions to COVID-19 crisis management. With publication first sequences SARS-CoV-2, several diagnostic PCR assays have been developed and published. In addition in-house market was flooded with numerous commercially available ready-to-use kits, both approaches showing alarming shortages in reagent supply.
Human Ebola infection is characterized by a paralysis of the immune system. A signature αβ T cells in fatal has been recently proposed, while involvement innate protection/pathogenesis unknown. Aim this study was to analyze γδ and NK patients from outbreak 2014-2015 occurred West Africa, assess their association with clinical outcome.Nineteen Ebola-infected were enrolled at time admission Treatment Centre Guinea. Patients divided two groups on basis outcome. The analysis performed using...
Since the emergence of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) in December 2019, scientific community has been sharing data on epidemiology, diagnostic methods, and whole-genomic sequences almost real time. The latter have already facilitated phylogenetic analyses, transmission chain tracking, protein modeling, identification possible therapeutic targets, timely risk assessment, novel variants. We established evaluated an amplification-based approach for whole-genome...
Poxviruses are known to evolve slower than RNA viruses with only 1-2 mutations/genome/year. Rather single mutations, rearrangements such as gene gain and loss, which have been discussed a possible driver for host adaption, were described in poxviruses. In 2022 2023 the world is being challenged by largest global outbreak so far of Mpox virus, virus seems established itself human community an extended period time. Here, we report five genomes from Germany extensive duplication leading...
We present an amplicon-based assay for MinION Nanopore sequencing of mpox virus (MPXV) genomes from clinical specimens, obtaining high-quality results with average genome coverage 99% Ct values up to 25, and a 97.1% 25 30 which are challenging sequence. This is easy implement in PCR-based workflows provides accurate genomic data within short time.
<title>Abstract</title> Mpox denotes a viral zoonosis caused by monkeypox virus (MPXV), which is endemic in West and Central Africa. In spring 2022, notable outbreaks of mpox clade IIb were recorded several high-income countries, predominantly affecting men who have sex with (MSM). At the peak outbreak, over 200 new cases per week reported Berlin, constitutes one largest MSM communities Europe. Within same year, outbreak virtually disappeared, it unclear factors contributed to its rapid...
Abstract Background In 2015, the laboratory at Ebola treatment center in Coyah, Guinea, confirmed virus disease (EVD) 286 patients. The cycle threshold (Ct) of an virus–specific reverse transcription–polymerase chain reaction assay and 13 blood chemistry parameters were measured on admission during hospitalization. Favipiravir was offered to patients with EVD a compassionate-use basis. Methods To reduce biases raw field data, we carefully selected 163 for retrospective study assess...
Before the international spread of monkeypox in May 2022, PCR kits for detection orthopoxviruses, and specifically virus, were rarely available. Here we describe evaluation 11 recently developed commercially available virus DNA. All tested are currently intended research use only clinical performance still needs to be assessed more detail, but all suitable diagnostics with variations specificity rather than sensitivity.
IL-2 secretion in response to mitogenic stimulation, assayed vitro, is significantly reduced circulating T lymphocytes isolated from healthy old people, but the significance of this abnormality and how it relates vivo remain unclear. We found that PHA plus PMA by peripheral blood cells 10 out 32 (31%) individuals (mean age 86 yr, range 74-97) was decreased compared with results obtained 23 younger 34 23-46). This defect vitro reversible after a 3-day incubation absence activators. The who...
Pre-vaccine SARS-CoV-2 seroprevalence data from Germany are scarce outside hotspots, and socioeconomic disparities remained largely unexplored. The nationwide representative RKI-SOEP study (15,122 participants, 18-99 years, 54% women) investigated testing in a supplementary wave of the Socio-Economic-Panel conducted predominantly October-November 2020. Self-collected oral-nasal swabs were PCR-positive 0.4% Euroimmun anti-SARS-CoV-2-S1-IgG ELISA dry-capillary-blood antibody-positive 1.3% (95%...
Abstract The monkeypox virus (MPXV) outbreak in 2022 has renewed interest the detection of antibodies against orthopox viruses (OPXV) and MPXV, as serological methods can aid diagnostics are key to epidemiological studies. Here three complementary described with different strengths development evaluation in‐house assays: An immunofluorescence assay (IFA) for specific IgG IgM, an enzyme‐linked immunosorbent higher sample throughput studies a neutralization test detect neutralizing antibodies....
Abstract Background Studies have shown that Omicron breakthrough infections can occur at higher SARS-CoV-2 antibody levels compared to previous variants. Estimating the magnitude of immunological protection induced from COVID-19 vaccination and infection remains important due varying local pandemic dynamics types programmes, particularly among at-risk populations such as health care workers (HCWs). We analysed a follow-up serological survey HCWs tertiary referral hospital in Germany...
During the mpox outbreak in 2022, highest number of cases Germany were registered Berlin, almost all them men who have sex with (MSM). However, frequency clinically undiagnosed infections is unknown.
Non-pharmaceutical interventions (NPIs) remain decisive tools to contain SARS-CoV-2. Strategies that combine NPIs with testing may improve efficacy and shorten quarantine durations. We developed a stochastic within-host model of SARS-CoV-2 captures temporal changes in test sensitivities, incubation periods, infectious periods. used the simulate relative transmission risk for (1) isolation symptomatic individuals, (2) contact person management, (3) incoming travelers. estimated travelers at...
Background: Testing of possibly infected individuals remains cornerstone containing the spread SARS-CoV-2. Detection dogs could contribute to mass screening. Previous research demonstrated canines' ability detect SARS-CoV-2-infections but has not investigated if can differentiate between COVID-19 and other virus infections. Methods: Twelve were trained SARS-CoV-2 positive samples. Three test scenarios performed evaluate their discriminate from viral infections a different aetiology. Naso-...