A5001860516- Epilepsy research and treatment
- Human-Animal Interaction Studies
- SARS-CoV-2 detection and testing
- Advanced Chemical Sensor Technologies
- Infant Health and Development
- SARS-CoV-2 and COVID-19 Research
- Neuroscience and Neuropharmacology Research
- Biosensors and Analytical Detection
- Diet and metabolism studies
- Olfactory and Sensory Function Studies
- Epigenetics and DNA Methylation
- Veterinary Pharmacology and Anesthesia
- Gut microbiota and health
- Veterinary Orthopedics and Neurology
- Prostate Cancer Treatment and Research
- Pharmacological Effects and Toxicity Studies
- COVID-19 diagnosis using AI
- Neuroendocrine regulation and behavior
- Medical Imaging and Analysis
- Dermatology and Skin Diseases
- Cancer, Hypoxia, and Metabolism
- Ocular Surface and Contact Lens
- Renal and related cancers
- 3D Shape Modeling and Analysis
- Hemoglobin structure and function
University of Veterinary Medicine Hannover, Foundation
2017-2025
University of Veterinary Medicine
2021-2023
University Hospital Bonn
2013-2017
Max Planck Institute for Plant Breeding Research
2010
Friedrich-Alexander-Universität Erlangen-Nürnberg
2004-2005
Forschungsinstitut für Anwendungsorientierte Wissensverarbeitung
1997-1999
Fraunhofer Society
1997
As the COVID-19 pandemic continues to spread, early, ideally real-time, identification of SARS-CoV-2 infected individuals is pivotal in interrupting infection chains. Volatile organic compounds produced during respiratory infections can cause specific scent imprints, which be detected by trained dogs with a high rate precision.
Formalin-fixed and paraffin-embedded (FFPE) tissues represent a valuable source for biomarker studies clinical routine diagnostics. However, they suffer from degradation of nucleic acids due to the fixation process. Since genetic epigenetic usually require PCR amplification, this hampers its use significantly, impairing robustness or necessitating short amplicons. In laboratory medicine highly robust performance is mandatory utility biomarkers. Therefore, methods improve using DNA FFPE...
DNA methylation analyses usually require a preceding bisulfite conversion of the DNA. The choice an appropriate kit for specific application should be based on performance requirements with regard to respective sample material. In this study, nine kits was evaluated: EpiTect Fast FFPE Bisulfite Kit, Kit (Qiagen), EZ Methylation-Gold Methylation-Direct Methylation-Lightning (Zymo Research), innuCONVERT All-In-One Basic Body Fluids (Analytik Jena). compared yield, degradation, purity,...
Integrated analysis of metabolomics, transcriptomics and immunohistochemistry can contribute to a deeper understanding biological processes altered in cancer possibly enable improved diagnostic or prognostic tests. In this study, set 254 metabolites was determined by gas-chromatography/liquid chromatography-mass spectrometry matched malignant non-malignant prostatectomy samples 106 prostate (PCa) patients. Transcription performed on 15 PCa patients using Affymetrix U133 Plus 2.0 arrays....
Abstract Manual tools for pain assessment from facial expressions have been suggested and validated several animal species. However, expression analysis performed by humans is prone to subjectivity bias, in many cases also requires special expertise training. This has led an increasing body of work on automated recognition, which addressed species, including cats. Even experts, cats are a notoriously challenging species assessment. A previous study compared two approaches ‘pain’/‘no pain’...
Pleural effusions (PE) are a common clinical problem. The discrimination between benign (BPE), malignant (MPE) and paramalignant (PPE) pleural is highly important to ensure appropriate patient treatment. Today, cytology the gold standard for diagnosing effusions. However, its sensitivity limited due sometimes low abundance of tumor cells challenging assessment cell morphology in cytological samples. This study aimed develop validate diagnostic test, which allows specific detection based on...
The main strategy to contain the current SARS-CoV-2 pandemic remains implement a comprehensive testing, tracing and quarantining until vaccination of population is adequate. Scent dogs could support testing strategies.
Introduction Anxiety and cognitive dysfunction are frequent, difficult to treat burdensome comorbidities in human canine epilepsy. Fecal microbiota transplantation (FMT) has been shown modulate behavior rodent models by altering the gastrointestinal (GIM). This study aims investigate beneficial effects of FMT on behavioral a translational model Methods Nine dogs with drug-resistant epilepsy (DRE) were recruited. The fecal donor had unremarkable behavior, which exhibited complete response...
Introduction Spectral detector Computed Tomography (SDCT) enables generation of virtual non-contrast (VNC) images derived from a post-contrast scan, as previously investigated in healthy dogs. This technique is especially promising for awake scanned rabbits where motion between the pre- and scans makes comparison challenging. study aimed to determine reliability VNC assessing abdominal organs 20 by evaluating their qualitative quantitative parameters compared true unenhanced (TUE) images....
Abstract Background Invertases are ubiquitous enzymes that irreversibly cleave sucrose into fructose and glucose. Plant invertases play important roles in carbohydrate metabolism, plant development, biotic abiotic stress responses. In potato ( Solanum tuberosum ), involved 'cold-induced sweetening' of tubers, an adaptive response to cold stress, which negatively affects the quality chips French fries. Linkage association studies have identified quantitative trait loci (QTL) for tuber sugar...
Molecular biomarkers may facilitate the distinction between aggressive and clinically insignificant prostate cancer (PCa), thereby potentially aiding individualized treatment. We analyzed cysteine dioxygenase 1 (CDO1) promoter methylation mRNA expression in order to evaluate its potential as prognostic biomarker. CDO1 were determined cell lines formalin-fixed paraffin-embedded prostatectomy specimens from a first cohort of 300 PCa patients using methylation-specific qPCR qRT-PCR. Univariate...
Circadian and other physiological rhythms play a key role in both normal homeostasis disease processes. Such is the case of circadian infradian seizure patterns observed epilepsy. In this paper we explore new implantable stimulator that implements chronotherapy as feedforward input to supplement open-loop closed-loop methods. This integrated algorithm allows for stimulation be adjusted ultradian, patients through slowly-varying temporal adjustments sub-components, while also enabling...
Introduction Alterations in the composition and function of gut microbiome have been reported idiopathic epilepsy (IE), however, interactions microbes with enteric nervous system (ENS) this context require further study. This pilot study examined how gastrointestinal microbiota (GIM), their metabolites, nutrients contained intestinal contents communicate ENS. Methods Fecal supernatants (FS) from healthy dogs IE, including drug-naïve, phenobarbital (PB) responsive, PB non-responsive dogs,...
Epilepsy may arise following acute brain insults, but no treatments exist that prevent epilepsy in patients at risk. Here we examined whether a combination of two glutamate receptor antagonists, NBQX and ifenprodil, acting different subtypes, exerts antiepileptogenic effects the intrahippocampal kainate mouse model epilepsy. These drugs were administered over 5 days kainate. Spontaneous seizures recorded by video/EEG intervals up to 3 months. Initial trials showed drug treatment during...
Cytology remains the gold standard for detection of malignant cells in ascites. However, its sensitivity is limited. The aim this study was to evaluate DNA methylation biomarkers differential diagnosis benign (ascites patients without malignancy), cancer directly caused by and paramalignant comorbidities but not malignancy) A cohort 283 (134 patients, 149 with diseases) presenting ascites prospectively enrolled. Ascites evaluated means cytopathological investigation SHOX2 SEPT9 cell-free...
Deep Learning based Convolutional Neural Networks (CNNs) are the state-of-the-art machine learning technique with medical image data. They have ability to process large amounts of data and learn features directly from raw Based on their training, these networks ultimately able classify unknown make predictions. Magnetic resonance imaging (MRI) is modality choice for many spinal cord disorders. Proper interpretation requires time expertise radiologists, so there great interest in using...
Background: Studies in people suggest that surface electroencephalography (EEG) electrode positions vary across participants and the consistency of these is electrode-, region-, examiner-dependent. The aim was to investigate variability EEG their underlying cortical regions (CRs) dogs using a neuronavigation system evaluate use said positioning, via cadaver study with 22 dogs. CT scans MRI were performed for each dog. These uploaded onto where desired CRs annotated. marked on heads, which...
Background: Testing of possibly infected individuals remains cornerstone containing the spread SARS-CoV-2. Detection dogs could contribute to mass screening. Previous research demonstrated canines' ability detect SARS-CoV-2-infections but has not investigated if can differentiate between COVID-19 and other virus infections. Methods: Twelve were trained SARS-CoV-2 positive samples. Three test scenarios performed evaluate their discriminate from viral infections a different aetiology. Naso-...
Myoglobin (MB) is not only strongly expressed in myocytes, but also at much lower levels different cancer entities. 40% of breast tumors are MB-positive, with the globin being co-expressed markers tumor hypoxia a proportion cases. In cancer, MB expression associated positive hormone receptor status and patient prognosis. prostate another hormone-dependent type, 53% were recently shown to express MB. Especially more aggressive specimen correlates increased survival rates. Both findings might...
Poor medication compliance by human epilepsy patients is one of the leading causes treatment failure and increased seizure frequency. The aim this cross-sectional study was to analyse owner in pharmacological canine idiopathic identify factors associated with poor compliance.The number antiseizure drug tablets recorded determine if patient received sufficient cover time period between prescriptions assess compliant prescription cycles. Additionally, assessed an online survey owners.For...