A5046871399- Cancer, Hypoxia, and Metabolism
- CAR-T cell therapy research
- Hemoglobin structure and function
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Metabolism, Diabetes, and Cancer
- Erythrocyte Function and Pathophysiology
- Melanoma and MAPK Pathways
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Mass Spectrometry Techniques and Applications
- Mycorrhizal Fungi and Plant Interactions
- Cutaneous Melanoma Detection and Management
- Plant Pathogens and Fungal Diseases
- Lichen and fungal ecology
- Ion channel regulation and function
- Genomics and Phylogenetic Studies
- Muscle metabolism and nutrition
- Neuroscience of respiration and sleep
- Calcium signaling and nucleotide metabolism
- Nanoplatforms for cancer theranostics
- Monoclonal and Polyclonal Antibodies Research
- Machine Learning in Bioinformatics
- High Altitude and Hypoxia
- Bioinformatics and Genomic Networks
Johannes Gutenberg University Mainz
2014-2025
Roche (Switzerland)
2024
University Hospital Bonn
2024
University Medical Center of the Johannes Gutenberg University Mainz
2022-2023
Klinik und Poliklinik für Psychosomatische Medizin und Psychotherapie
2020
Melanoma often recurs after a latency period of several years, presenting T cell-edited phenotype that reflects role for CD8(+) cells in maintaining metastatic latency. Here, we report an investigation patient with multiple recurrent lesions, where poorly immunogenic melanoma phenotypes were found to evolve the presence autologous tumor antigen-specific cells. from two three late metastases, developing within 6-year period, lacked HLA class I expression. cell-resistant, I-negative became...
Lichens are valuable models in symbiosis research and promising sources of biosynthetic genes for biotechnological applications. Most lichenized fungi grow slowly, resist aposymbiotic cultivation, poor candidates experimentation. Obtaining contiguous, high-quality genomes such symbiotic communities is technically challenging. Here, we present the first assembly a lichen holo-genome from metagenomic whole-genome shotgun data comprising both PacBio long reads Illumina short reads. The nuclear...
In the subterranean rodent (Nanno)spalax galili, evolutionary adaptation to hypoxia is correlated with longevity and tumor resistance. Adapted gene-regulatory networks of Spalax might pinpoint strategies maintain health in humans. Comparing liver, kidney spleen transcriptome data from rat at normoxia, we identified differentially expressed gene pathways common multiple organs both species. Body-wide interspecies differences affected processes like cell death, antioxidant defense, DNA repair,...
Recently, immunohistochemical analysis of myoglobin (MB) in human breast cancer specimens has revealed a surprisingly widespread expression MB this nonmuscle context. The positive correlation with hypoxia-inducible factor 2α (HIF-2α) and carbonic anhydrase IX suggested that oxygen regulates carcinomas. Here, we report mRNA protein levels are robustly induced by prolonged hypoxia cell lines, part via HIF-1/2-dependent transactivation. hypoxia-induced originated from novel alternative...
Abstract The blind subterranean mole rat Spalax shows a remarkable tolerance to hypoxia, cancer-resistance and longevity. Unravelling the genomic basis of these adaptations will be important for biomedical applications. RNA-Seq gene expression data were obtained from normoxic hypoxic liver tissue. Hypoxic broadly downregulates genes major function pathways. This energy-saving response is likely crucial adaptation low oxygen levels. In contrast, hypoxia-sensitive massive upregulation energy...
// Barbara Schrörs 1 , Silke Lübcke Volker Lennerz Martina Fatho Anne Bicker 2 Catherine Wölfel Patrick Derigs Thomas Hankeln Dirk Schadendorf 3 Annette Paschen Internal Medicine III, University Cancer Center (UCT) and Research for Immunotherapy (FZI), Medical (UMC) of the Johannes Gutenberg German Consortium (DKTK), Partner Site Frankfurt/Mainz, Mainz, Germany Institute Molecular Genetics, University, Department Dermatology, Hospital, Duisburg-Essen Essen/Düsseldorf, Essen, Correspondence...
Myoglobin (MB) is not only strongly expressed in myocytes, but also at much lower levels different cancer entities. 40% of breast tumors are MB-positive, with the globin being co-expressed markers tumor hypoxia a proportion cases. In cancer, MB expression associated positive hormone receptor status and patient prognosis. prostate another hormone-dependent type, 53% were recently shown to express MB. Especially more aggressive specimen correlates increased survival rates. Both findings might...
The identification of novel physiological regulators that stimulate energy expenditure through brown adipose tissue (BAT) activity in substrate catalysis is utmost importance to understand and treat metabolic diseases. Myoglobin (MB), known store or transport oxygen heart skeletal muscles, has recently been found bind fatty acids with constants its oxygenated form (i.e., MBO2). Here, we investigated the vivo effect MB expression on BAT activity. In particular, studied mitochondrial function...
Myoglobin (MB) is an oxygen-binding protein usually found in cardiac myocytes and skeletal muscle fibers. It may function as a temporary storage transport for O 2 but could also have scavenging capacity reactive oxygen nitrogen species. In addition, MB has recently been identified hallmark luminal breast cancer was shown to be robustly induced under hypoxia. Cellular responses hypoxia are regulated by the transcription factor hypoxia-inducible (HIF). For exploring of cancer, we employed...
Recently, the ectopic expression of myoglobin (MB) was reported in human epithelial cancer cell lines and breast tumor tissues, where MB increased with hypoxia. The better prognosis MB-positive patients suggested that globin exerts a tumor-suppressive role, possibly by impairing mitochondrial activity hypoxic carcinoma cells. To understand gene regulation cancer, we systematically investigated architecture gene, its transcripts promoters. In silico analysis transcriptome data from normal...
Myoglobin (MB) is known to bind and deliver oxygen in striated muscles at high expression levels. MB also expressed much reduced levels mammary epithelial cells, where the protein´s function unclear. In this study, we aim determine whether impacts fatty acid trafficking facilitates aerobic ß-oxidation cells. We utilized MB-wildtype versus MB-knockout mice human breast cancer cells examine impact of its oxygenation status on metabolism mouse milk epithelia. deficient were generated through...
Ectopic myoglobin (MB) expression, mediated by alternative and hypoxia-inducible transcription, has recently been demonstrated in several epithelial tumours. This study aimed to examine the expression of MB hormone-independent head neck squamous cell carcinomas (HNSCCs).Using imunohistochemistry, ectopic was analyzed on tissue microarrays (TMAs) 524 patients with localized locally advanced primary recurrent HNSCC who had undergone surgical treatment curative intent. Associations survival...
The muscle‑associated respiratory protein myoglobin (MB) is expressed in multiple types of cancer, including breast and prostate tumors. In Kaplan‑Meier analyses the two tumor types, MB positivity associated with favorable prognoses. Despite its well‑characterized function myocytes, role cancer remains unclear. To study impact endogenous expression, small interfering RNA MB‑knockdown cells were engineered using breast, colon cell lines (MDA‑MB468, LNCaP, DLD‑1), their transcriptomes...
Abstract Myoglobin (MB) is expressed in different cancer types and may act as a tumor suppressor breast cancer. The mechanisms by which basal MB expression level impacts murine mammary tumorigenesis are unclear. We investigated how influences proliferation, metastasis, hypoxia, chemotherapy treatment vivo . crossed PyMT WapCreTrp53 flox mouse models that differed grade/type onset of carcinoma with knockout mice. loss WapCre;Trp53 mice did not affect development progression. On the other...
Inflammatory bowel disease such as chronic colitis promotes colorectal cancer, which is a common cause of cancer mortality worldwide. Hypoxia characteristic inflammation well solid tumors and enforces gene expression response controlled by hypoxia-inducible factors (HIFs). Once established, are immunosuppressive to escape their abatement through immune cells. Although HIF activity known 1) promote development 2) drive tumor suppression the secretion adenosine, both prolyl hydroxylases an...
The expression of myoglobin (MB), well known as the oxygen storage and transport protein myocytes, is a novel hallmark luminal subtype in breast cancer patients correlates with better prognosis. mechanisms by which MB impacts mammary tumorigenesis are hitherto unclear. We aimed to unravel this role using CRISPR/Cas9 technology generate MB-deficient clones MCF7 SKBR3 cell lines subsequently characterize them transcriptomics plus molecular functional analyses. As main findings, loss at...
Abstract Expression of myoglobin (MB), well known as the oxygen storage and transport protein myocytes, is a novel hallmark luminal subtype in breast cancer patients correlates with better prognosis. The mechanisms by which MB impacts mammary tumorigenesis are hitherto unclear. We aimed to unravel this role, using CRISPR/Cas9 technology generate MB-deficient clones MCF7 SKBR3 cell lines subsequently characterize them transcriptomics plus molecular functional analyses. As main findings, loss...
Abstract Lichens are valuable models in symbiosis research and promising sources of biosynthetic genes for biotechnological applications. Most lichenized fungi grow slowly, resist aposymbiotic cultivation, generally poor candidates experimentation. Obtaining contiguous, high quality genomes such symbiotic communities is technically challenging. Here we present the first assembly a lichen holo-genome from metagenomic whole genome shotgun data comprising both PacBio long reads Illumina short...
<div>Abstract<p>Melanoma often recurs after a latency period of several years, presenting T cell–edited phenotype that reflects role for CD8<sup>+</sup> cells in maintaining metastatic latency. Here, we report an investigation patient with multiple recurrent lesions, where poorly immunogenic melanoma phenotypes were found to evolve the presence autologous tumor antigen–specific cells. Melanoma from two three late metastases, developing within 6-year period, lacked HLA...