A5037456516- Cancer, Hypoxia, and Metabolism
- Anesthesia and Neurotoxicity Research
- Toxic Organic Pollutants Impact
- Mitochondrial Function and Pathology
- High Altitude and Hypoxia
- Effects and risks of endocrine disrupting chemicals
- Hemoglobin structure and function
- Neuroscience and Neuropharmacology Research
- Carcinogens and Genotoxicity Assessment
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Erythropoietin and Anemia Treatment
- Cancer-related gene regulation
- Mesenchymal stem cell research
- Traumatic Brain Injury and Neurovascular Disturbances
- Muscle metabolism and nutrition
- Immune cells in cancer
- T-cell and B-cell Immunology
- Heme Oxygenase-1 and Carbon Monoxide
- Virus-based gene therapy research
- Neuroendocrine regulation and behavior
- Nitric Oxide and Endothelin Effects
- Nanoparticles: synthesis and applications
- Neurogenesis and neuroplasticity mechanisms
- RNA modifications and cancer
University of Duisburg-Essen
2017-2024
Witten/Herdecke University
2020-2024
Czech Academy of Sciences, Institute of Physiology
2022
Leibniz Institute of Environmental Medicine
2012-2014
Heinrich Heine University Düsseldorf
2009-2010
Friedrich Schiller University Jena
2005
Polybrominated diphenyl ethers (PBDEs) are persistent and bioaccumulative flame retardants, which found in rising concentrations human tissues. They of concern for health because animal studies have shown that they possess the potential to be developmentally neurotoxic.Because there is little knowledge effects PBDEs on brain cells, we investigated their toxic neural development vitro. Moreover, studied involvement thyroid hormone (TH) disruption caused by PBDEs.We used two PBDE congeners...
BackgroundDevelopmental neurotoxicity (DNT) of environmental chemicals is a serious threat to human health. Current DNT testing guidelines propose investigations in rodents, which require large numbers animals. With regard the “3 Rs” (reduction, replacement, and refinement) animal European regulation [Registration, Evaluation, Authorisation Chemicals (REACH)], alternative strategies are needed order refine reduce experiments allow faster less expensive screening.ObjectivesThe goal this study...
Because of their lipophilicity, persistent organic pollutants (POPs) cross the human placenta, possibly affecting central nervous system development. Most POPs are known aryl hydrocarbon receptor (AhR) ligands and activators AhR signaling. Therefore, activation has been suggested to cause developmental neurotoxicity (DNT).We studied effects on basic processes brain development in two comparative vitro systems determine whether AhR-activation is underlying mechanism for reported DNT humans.We...
Fluorescence imaging of green fluorescent protein (GFP) may be used to locate proteins in live cells and fluorescence lifetime (FLIM) employed probe the local microenvironment proteins. Here we apply FLIM GFP-tagged at cell surface an inhibitory natural killer (NK) immunological synapse (IS). We present a novel quantitative analysis images that believe is useful determine whether apparent heterogeneity statistically significant. observe that, although variation observed close expected...
Abstract Sufficient tissue oxygenation is required for regular brain function; thus oxygen supply must be tightly regulated to avoid hypoxia and irreversible cell damage. If occurs the transcription factor complex hypoxia-inducible (HIF) will accumulate coordinate adaptation of cells hypoxia. However, even under atmospheric O 2 conditions stabilized HIF-2α protein was found in brains adult mice. Mice with a neuro-specific knockout Hif-2α showed reduction pyramidal neurons retrosplenial...
Myoglobin (MB) is an oxygen-binding protein usually found in cardiac myocytes and skeletal muscle fibers. It may function as a temporary storage transport for O 2 but could also have scavenging capacity reactive oxygen nitrogen species. In addition, MB has recently been identified hallmark luminal breast cancer was shown to be robustly induced under hypoxia. Cellular responses hypoxia are regulated by the transcription factor hypoxia-inducible (HIF). For exploring of cancer, we employed...
Artificial blood for clinical use is not yet available therefore, we previously developed artificial oxygen carriers (capsules) and showed their functionality in vitro biocompatibility vivo. Herein, assessed the of capsules vivo a normovolemic hemodilution rat-model. We stepwise exchanged male Wistar-rats with medium either presence (treatment) or absence (control). investigated tissue hypoxia thoroughly through online biomonitoring, determination enzyme activity pancreatic hormones plasma,...
The transcription factor Hypoxia-inducible 1 (HIF-1) plays a pivotal role in tissue regeneration. HIF-1 is negatively controlled by O2-dependent prolyl hydroxylases with predominant of hydroxylase 2 isoform (Phd2). Transgenic mice, hypomorphic for this isoform, accumulate more under normoxic conditions. Using these we investigated the influence Phd2 and on regenerative capability skeletal muscle after myotrauma. Phd2-hypomorphic wild type mice (on C57Bl/6 background) were grouped...
ABSTRACT During acute viral infections, innate immune cells invade inflamed tissues and face hypoxic areas. Hypoxia-inducible factors (HIFs) adapt cellular responses towards these conditions. We wanted to investigate the effects of a loss HIF-2α in macrophages during Friend murine leukemia retrovirus (FV) infection C57BL/6 mice using Cre/loxP system. Remarkably, with floxed Hif-2a (Hif-2afl; is also known as Epas1) did not show any signs FV independent Cre activity. This prevented detailed...
Abstract HIF-2 represents a tissue-specific isoform of the hypoxia-inducible factors (HIFs) which regulate oxygen homeostasis in cell. In acute deficiency, HIF transcription ensure timely restoration adequate supply. Particularly medical conditions such as stroke, have high mortality risk due to ischaemic brain damage, rapid recovery supply is extraordinary importance. Nevertheless, endogenous mechanisms are often not sufficient respond severe hypoxic stress with restoring oxygenation and...
Macrophages are the frontline of defence against foreign microorganisms, including bacteria, parasites, and viruses. During acute viral infection, macrophages must invade inflamed tissue toward low oxygen concentrations, where genetic cellular responses depend on hypoxia-inducible factors (HIF). In study reported here we investigated role HIF-1α in macrophage function during retroviral infection. Wild-type myeloid cell-specific knockout mice were infected with Friend retrovirus (FV), immune...
The tumour suppressor von Hippel-Lindau (VHL) is involved in ubiquitination and subsequent proteasomal degradation of hypoxia-inducible factors (HIFs). Under hypoxic conditions, HIFα-subunits are stabilized, leading to dimerization with HIFβ transactivation genes for metabolism, survival angiogenesis. Human renal cancer cells A498 VHL-deficient harbor stabilized HIF-2, whereas Caki-1 VHL-intact HIF-1-dominant. Previous studies demonstrated increased paired-like homeodomain transcription...