A5064823386- Metabolomics and Mass Spectrometry Studies
- Liver Disease Diagnosis and Treatment
- Pancreatic and Hepatic Oncology Research
- Pancreatitis Pathology and Treatment
- Cancer, Hypoxia, and Metabolism
- Photosynthetic Processes and Mechanisms
- Cancer, Lipids, and Metabolism
- Metabolism and Genetic Disorders
- Algal biology and biofuel production
- Advanced Proteomics Techniques and Applications
- Diet and metabolism studies
- Plant Molecular Biology Research
- Adipose Tissue and Metabolism
- Clinical Laboratory Practices and Quality Control
- Alcohol Consumption and Health Effects
- Mass Spectrometry Techniques and Applications
- Muscle metabolism and nutrition
- Caveolin-1 and cellular processes
- Hepatocellular Carcinoma Treatment and Prognosis
- Fatty Acid Research and Health
- Gut microbiota and health
- Bioinformatics and Genomic Networks
- Light effects on plants
- Microbial bioremediation and biosurfactants
- Endoplasmic Reticulum Stress and Disease
Universitätsmedizin Greifswald
2023
Syneos Health (Germany)
2009-2021
German Institute of Human Nutrition
1997-2000
University of Göttingen
1992-1993
Abstract Background Genome-wide transcript profiling and analyses of enzyme activities from central carbon nitrogen metabolism show that levels undergo marked rapid changes during diurnal cycles after transfer to darkness, whereas in are smaller delayed. In the starchless pgm mutant, where sugars depleted every night, there accentuated levels. Enzyme this mutant do not larger changes; instead, they shift towards found wild type several days darkness. This indicates change slowly, integrating...
Summary Arabidopsis seedlings were subjected to 2 days of carbon starvation, and then resupplied with 15 m sucrose. The transcriptional metabolic response was analyzed using ATH1 arrays, real‐time quantitative (q)RT‐PCR analysis >2000 transcription regulators, robotized assays enzymes from central metabolism metabolite profiling. Sucrose led within 30 min greater than threefold changes the transcript levels for >100 genes, including 20 ubiquitin‐targeting proteins, four trehalose...
Objective Current non-invasive diagnostic tests can distinguish between pancreatic cancer (pancreatic ductal adenocarcinoma (PDAC)) and chronic pancreatitis (CP) in only about two thirds of patients. We have searched for blood-derived metabolite biomarkers this purpose. Design For a case–control study three tertiary referral centres, 914 subjects were prospectively recruited with PDAC (n=271), CP (n=282), liver cirrhosis (n=100) or healthy as well non-pancreatic disease controls (n=261)...
ABSTRACT This paper characterizes the transcriptional and metabolic response of a chilling‐tolerant species to an increasingly large decrease temperature. Arabidopsis Col‐0 was grown at 20 °C transferred 17, 14, 12, 10 or 8 for 6 78 h, before harvesting rosette profiling >22 000 transcripts, >20 enzyme activities >80 metabolites. Most parameters showed qualitatively similar across entire temperature range, with amplitude increasing as decreased. Transcripts typically changes after...
Metabolomics is a valuable tool with applications in almost all life science areas. There an increasing awareness of the essential need for high-quality biospecimens studies applying omics technologies and biomarker research. Tools to detect effects both blood plasma processing are key assuring reproducible credible results. We report on response human metabolome common preanalytical variations comprehensive metabolomics analysis reveal such markers.Human EDTA was subjected while being...
Dietary preferences influence basal human metabolism and gut microbiome activity that in turn may have long-term health consequences. The present study reports the metabolic responses of free living subjects to a daily consumption 40 g dark chocolate for up 14 days. A clinical trial was performed on population 30 subjects, who were classified low high anxiety traits using validated psychological questionnaires. Biological fluids (urine blood plasma) collected during 3 test days at beginning,...
High-quality biological samples are required for the favorable outcome of research studies, and valid data sets crucial successful biomarker identification. Prolonged storage biospecimens may have an artificial effect on compound levels. In order to investigate potential effects long-term metabolome, human ethylenediaminetetraacetic acid (EDTA) plasma stored up 16 years were analyzed by gas liquid chromatography-tandem mass spectrometry-based metabolomics. Only 2% 231 tested metabolites...
Sarcosine in prostate cancer tissue samples was recently reported to be increased during progression metastasis and suggested a key metabolite of cell invasion aggressiveness. We reevaluated sarcosine as potential indicator tumor aggressiveness, predictor recurrence-free survival.Sarcosine matched malignant nonmalignant from 92 patients with after radical prostatectomy measured the framework global profiling study by gas chromatography/mass spectrometry. related results age, volume, stage,...
Integrated analysis of metabolomics, transcriptomics and immunohistochemistry can contribute to a deeper understanding biological processes altered in cancer possibly enable improved diagnostic or prognostic tests. In this study, set 254 metabolites was determined by gas-chromatography/liquid chromatography-mass spectrometry matched malignant non-malignant prostatectomy samples 106 prostate (PCa) patients. Transcription performed on 15 PCa patients using Affymetrix U133 Plus 2.0 arrays....
Objective Chronic pancreatitis (CP) is a fibroinflammatory syndrome leading to organ dysfunction, chronic pain, an increased risk for pancreatic cancer and considerable morbidity. Due lack of specific biomarkers, diagnosis based on symptoms but insensitive imaging features, preventing early appropriate management. Design We conducted type 3 study multivariable prediction individual prognosis according the TRIPOD guidelines. A signature distinguish CP from controls (n=160) was identified...
Metabolomic research offers a deeper insight into biochemical changes in cancer metabolism and is promising tool for identifying novel biomarkers. We aimed to evaluate the diagnostic prognostic potential of metabolites prostate (PCa) tissue after radical prostatectomy. In matched malignant nonmalignant prostatectomy samples from 95 PCa patients, aminoadipic acid, cerebronic gluconic glycerophosphoethanolamine, 2-hydroxybehenic isopentenyl pyrophosphate, maltotriose, 7-methylguanine...
Pancreatic ductal adenocarcinoma cancer (PDAC) is a highly lethal malignancy requiring efficient detection when the primary tumor still resectable. We previously developed MxPancreasScore comprising 9 analytes and serum carbohydrate antigen 19-9 (CA19-9), achieving an accuracy of 90.6%. The necessity for 5 different analytical platforms multiple runs, however, hindered clinical applicability. therefore aimed to develop simpler single-analytical run, single-platform diagnostic signature.We...
Metabolomics is a powerful technology with broad applications in life science that, like other -omics approaches, requires high-quality samples to achieve reliable results and ensure reproducibility. Therefore, along quality assurance, methods assess sample regarding pre-analytical confounders are urgently needed. In this study, we analyzed the response of human serum metabolome variations comprising prolonged blood incubation extended storage at room temperature by using gas...
Two gram-positive, strictly anoxic, coccoid- to rod-shaped strains of bacteria, Clostridium coccoides 1410 and C. 3110, were isolated from human feces on the typical homoacetogenic substrates formate plus H2 CO2 (strain 1410) vanillate 3110) in presence 2-bromoethanesulfonate inhibit methanogenesis. On basis 16S rRNA sequencing, DNA-DNA hybridization, physiological morphological parameters, both isolates are closely related DSM 935T. The G+C contents DNA 46.1 46.2 mol% for respectively....
The purpose of the present study was first to identify drivers variance in plasma metabolite profiles cats and dogs that may affect interpretation nutritional metabolomic studies. A total fourteen housed environmentally enriched accommodation were fed a single batch diet maintain body weight. Fasting blood samples taken on days 14, 16 18 study. Gas chromatography–mass spectrometry (GC–MS), liquid chromatography (LC)–MS/MS solid-phase extraction–LC–MS/MS analyses used for profiling. Principal...
Discrepancies in blood sample collection and processing could have a significant impact on levels of metabolites, peptides, protein biomarkers inflammation the blood; thus, quality control is critical for successful biomarker identification validation. In this study, we analyzed effects several preanalytical conditions, including different storage times temperatures or plasma samples centrifugation forces human using ethylenediaminetetraacetic acid (EDTA) as an anticoagulant. Temperature was...
Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers. Developing biomarkers for early detection and chemotherapeutic response prediction crucial to improve dismal prognosis PDAC patients. However, molecular cancer signatures based on transcriptome analysis do not reflect intratumoral heterogeneity. To explore a more accurate stratification phenotypes in an easily accessible matrix, plasma metabolome using MxP® Global Profiling Lipidomics was performed 361 We identified...
Methanosarcina strain Gö1 was tested for the presence of cytochromes. Low-temperature spectroscopy, hemochrome derivative and redox titration revealed two b-type (b559 b564) c-type (c547 c552) cytochromes in membranes from Gö1. The midpoint potentials determined were Em,7 = -135 +/- 5 -240 11 mV (b-type cytochromes) -140 10 -230 (c-type cytochromes). protoheme IX heme c contents 0.21 to 0.24 0.09 0.28 mumol/g membrane protein, respectively. No detectable cytoplasmic fraction. Of various...
Variable processing and storage of whole blood and/or plasma are potential confounders in biomarker development clinical assays. The goal the study was to investigate how pre-analytical variables impact human proteome. Whole obtained from 16 apparently healthy individuals collected six EDTA tubes processed randomly under variable conditions including at 0 °C or RT for 6 h (B6h0C B6hRT) before plasma, 4 24 (P24h4C P24hRT), low centrifugal force 1300 × g, (Low×g), immediate 2500 g (control)...
Introduction Pancreatic ductal adenocarcinoma (PDAC) has a dismal prognosis with an overall 5-year survival of approximately 8%. The success in reducing the mortality rate PDAC is related to discovery new therapeutic agents, and significant extent development early detection prevention programmes. Patients new-onset diabetes mellitus (DM) represent high-risk group for as they have eightfold higher risk than general population. proposed screening programme may allow early, operable stage....
The homoacetogenic anaerobic bacterium Sporomusa sphaeroides was mutagenized with UV light. Taking advantage of the ampicillin enrichment technique and a newly developed test for detection heme in bacterial colonies, cytochrome-deficient mutant strain S. BK824 isolated. In contrast to wild type, this failed grow on betaine, betaine plus methanol, H2 CO2, methanol CO2. Growth formate, H2, pyruvate, acetoin not impaired. All enzymes Wood pathway as well hydrogenase carbon monoxide...