A5018310587- Respiratory viral infections research
- Influenza Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Pneumonia and Respiratory Infections
- Viral gastroenteritis research and epidemiology
- Health and Medical Studies
- Biosensors and Analytical Detection
- COVID-19 epidemiological studies
- Animal Disease Management and Epidemiology
- COVID-19 Clinical Research Studies
- Tracheal and airway disorders
- COVID-19 diagnosis using AI
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Congenital Diaphragmatic Hernia Studies
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Respiratory Support and Mechanisms
- Travel-related health issues
- Infectious Encephalopathies and Encephalitis
- Viral Infectious Diseases and Gene Expression in Insects
- Disaster Response and Management
- vaccines and immunoinformatics approaches
- Cystic Fibrosis Research Advances
Robert Koch Institute
2015-2024
Hospital Clínico Universitario de Valladolid
2017
Leipzig University
2008
During the initial COVID-19 response, Germany's Federal Government implemented several nonpharmaceutical interventions (NPIs) that were instrumental in suppressing early exponential spread of SARS-CoV-2. NPI effect on transmission other respiratory viruses has not been examined at national level thus far.
Background Non-pharmaceutical interventions (NPIs) during the COVID-19 pandemic affected respiratory syncytial virus (RSV) circulation worldwide. Aim To describe, for children aged < 5 years, 2021 and 2022/23 RSV seasons in Germany. Methods Through data 16,754 specimens from outpatient sentinel surveillance, we investigated seasonality, circulating lineages, children’s age distributions 2022/23. Available information about disease severity hospital surveillance was analysed patients with...
Respiratory viruses are a cause of upper respiratory tract infections (URTI), but can be associated with severe lower (LRTI) in immunocompromised patients. The objective this study was to investigate the genetic variability influenza virus, parainfluenza virus and syncytial (RSV) duration viral shedding hematological Nasopharyngeal swabs from patients were screened for influenza, RSV on admission as well development symptoms. Consecutive collected until clearance. Out 672 tested patients,...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilises the angiotensin-converting enzyme (ACE2) transmembrane peptidase as cellular entry receptor. However, whether SARS-CoV-2 in alveolar compartment is strictly ACE2-dependent and to what extent virus-induced tissue damage and/or direct immune activation determines early pathogenesis still elusive.Spectral microscopy, single-cell/-nucleus RNA sequencing or ACE2 "gain-of-function" experiments were applied infected human lung...
ABSTRACT Since the 1970s, influenza B viruses have diverged into two antigenically distinct virus lineages called Yamagata and Victoria lineages. We present first real-time PCR assay for lineage differentiation to supplement classical antigenic analyses. The was successfully applied 310 primary samples collected in Germany from 2007 2009.
Essential epidemiologic and virologic parameters must be measured to provide evidence for policy/public health recommendations mathematical modeling concerning novel influenza A/H1N1 virus (NIV) infections. Therefore, from April through August of 2009, the authors collected nasopharyngeal specimens information on antiviral medication symptoms households with NIV infection a daily basis in Germany. Specimens were analyzed quantitatively by using reverse transcriptase-polymerase chain...
The reliable detection of SARS-CoV-2 has become one the most important contributions to COVID-19 crisis management. With publication first sequences SARS-CoV-2, several diagnostic PCR assays have been developed and published. In addition in-house market was flooded with numerous commercially available ready-to-use kits, both approaches showing alarming shortages in reagent supply.
Background The current spread of pandemic influenza A(H1N1)v virus necessitates an intensified surveillance infections worldwide. So far, in many laboratories routine diagnostics were limited to generic detection only. To provide interested with real-time PCR assays for type and subtype identification, we present a bundle which any human A B can be easily identified, including the virus. Principal Findings show optimal performance characteristics their validation on plasmids containing...
Based on our national outpatient sentinel surveillance, we have developed a novel approach to determine respiratory syncytial virus (RSV) epidemic seasons in Germany by using RSV positivity rate and its lower limit of 95% confidence interval. This method was evaluated retrospectively nine seasons, it is also well-suited describe off-season circulation near real time as observed for 2020/21 2021/22 during the COVID-19 pandemic. Prospective application crucial importance enable timely actions...
Abstract Background Comprehensive pathogen genomic surveillance represents a powerful tool to complement and advance precision vaccinology. The emergence of the Alpha variant in December 2020 resulting efforts track spread this other severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants concern led an expansion sequencing activities Germany. Methods At Robert Koch Institute (RKI), German National Public Health, we established Integrated Molecular Surveillance for SARS-CoV-2...
Before the COVID-19 pandemic, acute respiratory infections (ARIs) in children were mainly characterised by three pathogens: syncytial viruses (RSV), influenza and rhinoviruses. The impact of pandemic measures taken Germany (especially until end 2021) on incidence ARI adolescents aged 0 to 14 years pathogens causing them has not yet been comprehensively analysed.The evaluation is based data from population-based, virological hospital-based surveillance instruments up 2022.After onset early...
Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized. A patient (age group 5-15 years) displaying of hemolytic uremic syndrome died one day after hospitalization. qPCR, next generation sequencing, virus isolation, antigenic characterization, resistance analysis was performed and replication kinetics in well-differentiated airway cells were determined. Autopsy revealed...
Acute lower respiratory tract infection (ALRI) is a leading cause of childhood morbidity and mortality in developing countries. Globally, human syncytial virus (HRSV) the most common pathogen ALRI infants children. However, age-stratified HRSV disease burden data are largely absent from Africa, which key gap informing an evidence-based recommendation for introduction vaccine by WHO.
Importance In the context of emerging SARS-CoV-2 variants or lineages and new vaccines, it is key to accurately monitor COVID-19 vaccine effectiveness (CVE) inform vaccination campaigns. Objective To estimate vaccines administered in autumn winter 2022 2023 against symptomatic infection (with all circulating viruses XBB lineage particular) among people aged 60 years older Europe, compare different CVE approaches across exposed reference groups used. Design, Setting, Participants This...
Pigeon circovirus (PiCV) infection and young pigeon disease syndrome (YPDS), associated with high morbidity mortality, have been recognized in racing pigeons from large portions of Central Europe. There exist a number data indicating that YPDS is consequence PiCV subsequent immunosuppression. In order to prove be one the crucial factors YPDS, an experimental was performed under controlled conditions. Twenty-four domestic (Columba livia forma domestica) were divided into two groups 12 each;...
The corona virus disease-2019 (COVID-19) pandemic began in Wuhan, China, and quickly spread around the world. overlapped with two consecutive influenza seasons (2019/2020 2020/2021). This provided opportunity to study community circulation of viruses severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) outpatients infections during these within Bavarian Influenza Sentinel (BIS) Bavaria, Germany. From September March, oropharyngeal swabs collected at BIS were analysed for SARS-CoV-2...
A zoonotic A/sw/H1 av N1 1C.2.2 influenza virus infection was detected in a German child that presented with influenza-like illness, including high fever. There history of close contact pigs 3 days before symptom onset. The recovered within days. No other transmissions were observed. Serological investigations the isolate revealed cross-reactions ferret antisera against A(H1N1)pdm09 virus, indicating closer antigenic relationship than former seasonal H1N1 viruses.
IntroductionRecent data on influenza C virus indicate a possible higher clinical impact in specified patient populations than previously thought.AimWe aimed to investigate circulation Germany.MethodsA total of 1,588 samples from 0 4 year-old children presenting as outpatients with influenza-like illness (ILI) or acute respiratory infection were analysed retrospectively. The represented subset all the German national surveillance system for this age group 2012-14. presence was investigated by...
Human parainfluenza viruses (HPIVs) are important causes of respiratory illness, especially in young children. However, surveillance for HPIV is rarely performed continuously, and national-level epidemiologic genetic data scarce. Within the German sentinel system, to monitor acute infections (ARI), 4463 specimens collected from outpatients < 5 years age between October 2015 September 2019 were retrospectively screened 1-4 using real-time PCR. was identified 459 (10%) samples. HPIV-3 most...
ABSTRACT Respiratory syncytial virus (RSV) is a leading cause of acute lower respiratory tract infections causing significant morbidity and mortality among children the elderly; two RSV vaccines monoclonal antibody have recently been approved. Thus, there an increasing need for detailed continuous genomic surveillance circulating in resource-rich resource-limited settings worldwide. However, robust, cost-effective methods whole genome sequencing from clinical samples that are amenable to...
Using participatory, virologic, and wastewater surveillance systems, we estimated when to what extent reported data of adult COVID-19 cases underestimated incidence in Germany. We also examined how case underestimation evolved over time. Our findings highlight community-based systems can complement official notification for respiratory disease dynamics.