A5017118571- Bacillus and Francisella bacterial research
- Yersinia bacterium, plague, ectoparasites research
- Paleopathology and ancient diseases
- Bacteriophages and microbial interactions
- Congenital Heart Disease Studies
- Congenital heart defects research
- Microbial infections and disease research
- Forensic Entomology and Diptera Studies
- Poxvirus research and outbreaks
- Legionella and Acanthamoeba research
- Aquaculture disease management and microbiota
- Gut microbiota and health
- Forensic Anthropology and Bioarchaeology Studies
- MicroRNA in disease regulation
- Plant Pathogenic Bacteria Studies
- Brucella: diagnosis, epidemiology, treatment
- Hearing, Cochlea, Tinnitus, Genetics
- Electrocatalysts for Energy Conversion
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Phytochemistry and biological activities of Ficus species
- Leptospirosis research and findings
- Nanoporous metals and alloys
- Zoonotic diseases and public health
- Circular RNAs in diseases
Robert Koch Institute
2017-2023
Institut für Mikrobiologie der Bundeswehr
2023
Charité - Universitätsmedizin Berlin
2014-2020
German Centre for Cardiovascular Research
2020
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2020
University of California, Davis
2016-2019
Inserm
2014-2016
Institut de Recherche pour le Développement
2014-2016
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2014-2016
Centre National de la Recherche Scientifique
2014-2016
ABSTRACT Coprolites are fossilized fecal material that can reveal information about ancient intestinal and environmental microbiota. Viral metagenomics has allowed systematic characterization of viral diversity in human-associated specimens, but little is known the fossil remains. Here, we analyzed community a 14th-century coprolite from closed barrel Middle Ages site Belgium using electron microscopy metagenomics. Viruses infect eukaryotes, bacteria, archaea were detected, confirmed...
Paleomicrobiological investigations of a 14(th)-century coprolite found inside barrel in Namur, Belgium were done using microscopy, culture-dependent approach and metagenomics. Results confirmed by ad hoc PCR--sequencing. Investigations yielded evidence for flora from ancient environment preserved the coprolite, indicated microscopic observation amoebal cysts, plant fibers, seeds, pollens mold remains. Seventeen different bacterial species cultured mixing organisms known to originate be gut...
Nucleic-acid-based biosensors have enabled rapid and sensitive detection of pathogenic targets; however, these devices often require purified nucleic acids for analysis since the constituents complex biological fluids adversely affect sensor performance. This purification step is typically performed outside device, thereby increasing sample-to-answer time introducing contaminants. We report a novel approach using multifunctional matrix, nanoporous gold (np-Au), which enables both specific...
Burkholderia mallei is the etiological agent of glanders, a highly contagious and often fatal disease in equids. Due to high genetic clonality B. mallei, high-resolution typing assays are necessary differentiate between individual strains. Here we report on development validation robust reproducible core genome-based Multi Locus Sequence Typing Assay (cgMLST) for which based 3328 gene targets enables at strain level. The assay was validated using set 120 genomes from public databases 23...
Usher syndrome is an autosomal recessive disorder characterized both by deafness and blindness. For the three clinical subtypes of causal mutations in altogether 12 genes a modifier gene have been identified. Due to genetic heterogeneity syndrome, molecular analysis predestined for comprehensive parallelized all known next-generation sequencing (NGS) approaches. We describe here targeted enrichment deep exons compare costs workload this approach compared Sanger sequencing. also present...
Francisella tularensis is an intracellular pleomorphic bacterium and the causative agent of tularemia, a zoonotic disease with wide host range. Among F. subspecies, especially subsp. holarctica clinical relevance for European countries. The study presented herein focuses namely on genetic diversity spatial segregation in Germany, as still limited information available. investigation based analysis 34 isolates one draft genome from outbreak strain. were cultured sample material being that...
Abstract MicroRNAs (miRNAs) play an important role in guiding development and maintaining function of the human heart. Dysregulation miRNAs has been linked to various congenital heart diseases including Tetralogy Fallot (TOF), which represents most common cyanotic malformation humans. Several studies have identified dysregulated right ventricular (RV) tissues TOF patients. In this study, we profiled genome-wide whole transcriptome analyzed relationship mRNAs RV a homogeneous group 22...
A zoonotic A/sw/H1 av N1 1C.2.2 influenza virus infection was detected in a German child that presented with influenza-like illness, including high fever. There history of close contact pigs 3 days before symptom onset. The recovered within days. No other transmissions were observed. Serological investigations the isolate revealed cross-reactions ferret antisera against A(H1N1)pdm09 virus, indicating closer antigenic relationship than former seasonal H1N1 viruses.
Patient-specific induced pluripotent stem cells (ps-iPSCs) and their differentiated cell types are a powerful model system to gain insight into mechanisms driving early developmental disease-associated regulatory networks. In this study, we use ps-iPSCs insights Tetralogy of Fallot (TOF), which represents the most common cyanotic heart defect in humans. iPSCs were generated further cardiomyocytes (CMs) using standard methods from two well-characterized TOF patients healthy relatives serving...
Congenital heart defects (CHDs) are the most common birth defect in human with an incidence of almost 1% all live births. Most cases have a multifactorial origin both genetics and environment playing role its development progression. Adding epigenetic component to this aspect is exemplified by monozygotic twins which share same genetic background but different disease status. As result, interplay between genetic, environmental conditions might contribute etiology phenotype. To date,...
Global warming has caused an increase in the emergence of Vibrio species marine and estuarine environments as well fresh water bodies. Over past decades, antimicrobial resistance (AMR) evolved among toward various antibiotics commonly used for treatment infections. In this study, we assessed virulence patterns cholerae non-O1/non-O139 strains derived from Germany other European countries. A total 63 clinical 24 environmental strains, collected between 2011 2021, were analyzed. silico...
The cascade of innovations in biotechnology opens new pathways for biological warfare. international laboratory network being developed under the UN Secretary-General's Mechanism could provide vital evidence case an alleged attack.
Europe-wide activities to improve biosafety and biosecurity performed within the frameworks of European Union (EU)-funded Joint Actions EMERGE QUANDHIP led development an Integrated Checklist for Laboratory Biorisk Management (ECL).To better understand different approaches shaping biorisk management (BRM) systems on operational level in high containment laboratories, ECL was used map implementation BRM 32 laboratories 18 countries Europe. The results suggest that elements referring standard...
Francisella tularensis is the causative agent of tularemia, a zoonotic disease with wide host range. F. ssp. holarctica (Fth) clinical relevance for European countries, including Germany. Whole genome sequencing methods, canonical Single Nucleotide Polymorphism (canSNP) typing and whole SNP typing, have revealed that Fth strains belong to few monophyletic populations. The majority German isolates two basal phylogenetic clades B.6 (biovar I) B.12 II). Strains seem differ in their...
Cold-blooded hosts, particularly exotic frogs, have become a newly recognized reservoir for atypical Brucella species and strains worldwide, but their pathogenicity to humans remains largely unknown. Here we report the isolation molecular characterization of B. inopinata strain (FO700662) cultured from clinical samples taken captive diseased White's Tree Frog (Litoria caerulea) in Switzerland. The frog along with other reports human infection by raises question whether could pose risk health...
Here, we report the complete genome sequence of Francisella tularensis subsp. holarctica strain A271_1, isolated from a Eurasian beaver (Castor fiber) in 2012 Berlin/Brandenburg region, Germany.