A5010201733- Bacillus and Francisella bacterial research
- Bacteriophages and microbial interactions
- Poxvirus research and outbreaks
- Yersinia bacterium, plague, ectoparasites research
- Microbial Inactivation Methods
- Bacterial Genetics and Biotechnology
- Legionella and Acanthamoeba research
- Coral and Marine Ecosystems Studies
- Marine Biology and Ecology Research
- Microbial infections and disease research
- Polyomavirus and related diseases
- Mycotoxins in Agriculture and Food
- Marine Ecology and Invasive Species
- SARS-CoV-2 and COVID-19 Research
Robert Koch Institute
2018-2025
Museum für Naturkunde
2011
Humboldt-Universität zu Berlin
2011
The zoonotic and highly infectious pathogen Francisella tularensis is the etiological agent of tularemia. Tularemia in humans mainly caused by F. subspecies holarctica, but species like novicida, philomiragia, hispaniensis others are known to cause tularemia-like infections immunocompromised humans. In addition these species, further genera family Francisellaceae have been described, such as Allofrancisella, Parafrancisella Pseudofrancisella, less about distribution putative virulence...
Francisella tularensis is an intracellular pleomorphic bacterium and the causative agent of tularemia, a zoonotic disease with wide host range. Among F. subspecies, especially subsp. holarctica clinical relevance for European countries. The study presented herein focuses namely on genetic diversity spatial segregation in Germany, as still limited information available. investigation based analysis 34 isolates one draft genome from outbreak strain. were cultured sample material being that...
Here we present the characterization of a Francisella bacteriophage (vB_FhiM_KIRK) including morphology, genome sequence and induction prophage. The prophage (FhaGI-1) has previously been identified in F. hispaniensis strain 3523. UV radiation induced to assemble phage particles consisting an icosahedral head (~52 nm diameter), tail up 97 length mean width 9 nm. double stranded vB_FhiM_KIRK contains 51 open reading frames is 34,259 bp length. genotypic phylogenetic analysis indicated that...
Introduction Tularemia is mainly caused by Francisella tularensis ( Ft ) subsp. Ftt and holarctica in humans more than 200 animal species including rabbits hares. Human clinical manifestations depend on the route of infection range from flu-like symptoms to severe pneumonia with a mortality rate up 60% without treatment. So far, only 2D cell culture models are used study virulence , but gained results transferable human infections certain extent. Method In this study, we firstly established...
We recently identified and described a putative prophage on the genomic island FhaGI-1 located within genome of Francisella hispaniensis AS02-814 (F. tularensis subsp. novicida-like 3523). In this study, we constructed two variants phage integration vector, called pFIV1-Val pFIV2-Val (Francisella Integration Vector-tRNAVal-specific), using attL/R-sites site-specific integrase (FN3523_1033) FhaGI-1, chloramphenicol resistance cassette sacB gene for counter selection transformants against...
BackgroundIn 2016, an uncommon outbreak of oropharyngeal tularaemia involving six human cases occurred in Germany, caused by drinking contaminated fresh must after a grape harvest.AimWe describe the details laboratory investigations leading to identification strain, its characterisation next generation sequencing (NGS) and finding possible source contamination.MethodsWe incubated wine samples different media on agar plates. NGS was performed DNA isolated from young wine, sweet reserve case's...
Recently, a new environmental Francisella strain, sp. strain W12-1067, has been identified in Germany. This is negative for the pathogenicity island (FPI) but exhibits putative alternative type VI secretion system. Some known virulence factors of are present, pathogenic capacity this species not yet. In silico genome analysis reveals presence gene cluster tentatively enabling myo-inositol (MI) utilization via inositol oxygenase. Labelling experiments starting from 2H-inositol demonstrate...
Francisella tularensis is the causative agent of tularemia, a zoonotic disease with wide host range. F. ssp. holarctica (Fth) clinical relevance for European countries, including Germany. Whole genome sequencing methods, canonical Single Nucleotide Polymorphism (canSNP) typing and whole SNP typing, have revealed that Fth strains belong to few monophyletic populations. The majority German isolates two basal phylogenetic clades B.6 (biovar I) B.12 II). Strains seem differ in their...
The new species Seba henriki, collected in the waters around Norfolk Island is described. It differs from similar gruneri Yerman & Coleman, 2009 by expanded maxilliped palp articles 1 and 2; morphology of gnathopod which has a straight palm with 2 small notches inner margin dactylus; rather long ischium, propodus dactylus pereopod 6–7 basis ovoid produced posteroventral lobe surpassing ischium.