A5004703842- Bacteriophages and microbial interactions
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Plant Virus Research Studies
- Neuroscience and Neuropharmacology Research
- Schizophrenia research and treatment
- Nicotinic Acetylcholine Receptors Study
- Electroconvulsive Therapy Studies
- Chemical Synthesis and Analysis
- RNA and protein synthesis mechanisms
- Plant Disease Resistance and Genetics
- Microbial Community Ecology and Physiology
- SARS-CoV-2 detection and testing
- HIV Research and Treatment
- Photoreceptor and optogenetics research
- Pharmacovigilance and Adverse Drug Reactions
- Nanopore and Nanochannel Transport Studies
- Pharmaceutical Practices and Patient Outcomes
- Viral Infections and Immunology Research
- Psychiatric care and mental health services
- Sexual function and dysfunction studies
- Drug-Induced Adverse Reactions
- Bipolar Disorder and Treatment
- HIV/AIDS drug development and treatment
- DNA and Nucleic Acid Chemistry
Immanuel Krankenhaus
2019-2024
Robert Koch Institute
2022-2024
University College London
2014-2021
Klinikum Brandenburg
2018
Technical University of Darmstadt
2008-2017
Czech Academy of Sciences, Institute of Botany
2011
Abstract Edema as an adverse drug reaction is a commonly underestimated yet potentially debilitating condition. This study analyzes the incidence of severe psychotropic drug-induced edema (e.g., affecting face, legs, or multiple body parts and lasting for more than 1 week, in any case necessitating subsequent diuretic use) among psychiatric inpatients. The cases under examination are derived from observational pharmacovigilance program conducted German-speaking countries...
Influenza, caused by Influenza A and B viruses, represents a significant global health burden due to recurrent seasonal epidemics the risk of pandemics. To gauge large volume influenza cases, it may be helpful complement classical surveillance systems with additional approaches such as wastewater-based epidemiology (WBE), which can aid in early trend assessment epidemics. WBE has emerged promising tool for population-level surveillance, enabling detection viral nucleic acids wastewater...
The human glycine receptor (hGlyR) is an anion-permeable ligand-gated channel that part of a larger superfamily receptors called the Cys-loop family. hGlyRs are particularly amenable to single-channel recordings, thus making them model experimental system for understanding family in general. Understanding relationship between agonist binding and efficacy should improve our future prospects specific agonists or antagonists. However, at present, there no high-resolution structure complete...
Previous experiments established that when the unicellular green alga Chlorella NC64A is inoculated with two viruses, usually only one virus replicates in a single cell. That is, viruses mutually exclude another. In current study, we explore possibility virus-induced host membrane depolarization, at least partially caused by virus-encoded K(+) channel (Kcv), involved this mutual exclusion. Two chlorella PBCV-1 and NY-2A, were chosen for study because (i) they can be distinguished real-time...
Phycodnaviruses are large dsDNA, algal-infecting viruses that encode many genes with homologs in prokaryotes and eukaryotes. Among the viral gene products smallest proteins known to form functional K+ channels. To determine if these channels product of molecular piracy from their hosts, we compared sequences channel pore modules seven phycodnaviruses Chlorella variabilis Ectocarpus siliculosus, whose genomes have recently been sequenced. C. is host for two PBCV-1 NY-2A E. siliculosus virus...
ABSTRACT Paramecium bursaria chlorella virus 1 (PBCV-1) is the prototype of a family large, double-stranded DNA, plaque-forming viruses that infect certain eukaryotic chlorella-like green algae from genus Chlorovirus . PBCV-1 infection results in rapid host membrane depolarization and potassium ion release. One interesting feature chloroviruses they code for functional ion-selective channel proteins (Kcv) are considered responsible and, as consequence, efflux ions. This report examines...
Chlorella viruses are a source of interesting membrane transport proteins. Here we examine putative K(+) transporter encoded by virus FR483 and related chlorella viruses. The protein shares sequence structural features with HAK/KUP/KT-like transporters from plants, bacteria fungi. Yeast complementation assays Rb(+) uptake experiments show that the viral protein, termed HAKCV (high-affinity virus), is functional, characteristics similar to those known transporters. Expression studies revealed...
Some viruses have genes encoding proteins with membrane transport functions. It is unknown if these types of are rare or common in viruses. In particular, the evolutionary origin some viral obscure, where other homologs prokaryotic and eukaryotic organisms. We searched virus genomes databases looking for transmembrane possible function. This effort led to detection 18 different putative indicating that they not a rarity genomes. The most abundant K⁺ channels. Their predicted structures vary...
Abstract Hyponatremia (HN) is the most common electrolyte imbalance (defined as a serum sodium concentration Na(S) of < 130 mmol/l) and often induced by drugs including psychotropic drugs. AMSP (Arzneimittelsicherheit in der Psychiatrie) multicenter drug surveillance program that assesses severe or unusual adverse reactions (ADRs) occurring during treatment with This study presents data from 462,661 psychiatric inpatients treated participating hospitals between 1993 2016 serves an update...
Gating of ion channels is based on structural transitions between open and closed states. To uncover the chemical basis individual gates, we performed a comparative experimental computational analysis two K+ channels, KcvS KcvNTS. These small viral encoded channel proteins, with monomer size only 82 amino acids, resemble pore module all complex in terms structure function. Even though both proteins share about 90% acid sequence identity, they exhibit different probabilities ca. KcvNTS 40%...
Calcium-transporting ATPases (Ca2+ pumps) are major players in maintaining calcium homeostasis the cell and have been detected all cellular organisms. Here, we report identification of two putative Ca2+ pumps, M535L C785L, encoded by chlorella viruses MT325 AR158, respectively, functional characterization M535L. Phylogenetic sequence analyses place viral proteins group IIB P-type even though they lack a typical feature this class, calmodulin-binding domain. A pump gene is present 45 47...
Using participatory, virologic, and wastewater surveillance systems, we estimated when to what extent reported data of adult COVID-19 cases underestimated incidence in Germany. We also examined how case underestimation evolved over time. Our findings highlight community-based systems can complement official notification for respiratory disease dynamics.
Objective: Priapism is a rare adverse effect of several psychotropics. Both, typical and atypical antipsychotics, as well trazodone are known to cause priapism. The mechanism still not fully understood, however, the most common assumption that priapism occurs due α-adrenergic blocking effects drugs. Methods: Here we present from AMSP database 19 cases being likely caused by variety We further reviewed case reports in order find similarities identify risk factors. Results: Several patterns...
Summary The glycine receptor is a pentameric, neurotransmitter-activated ion channel that transitions between closed/resting, open and desensitized states. Glycine, full agonist, produces an probability (Po) of ∼1.0 while partial agonists, such as taurine γ-amino butyric acid (GABA) yield submaximal Po values. Despite extensive studies pentameric Cys-loop receptors, there little knowledge the molecular mechanisms underpinning agonist action how from closed to conformations. Here we use...
Glycine receptors (GlyR) belong to the pentameric ligand-gated ion channel (pLGIC) superfamily and mediate fast inhibitory transmission in vertebrate CNS. Disruption of glycinergic by inherited mutations produces startle disease man. Many are GlyRs provide useful clues function domains. E103K is one few found extracellular agonist binding site channel, loop A principal side subunit interface. Homology modeling shows that chain Glu-103 close Arg-131, E complementary site, may form a salt...
Transmembrane domains (TMDs) are often flanked by Lys or Arg because they keep their aliphatic parts in the bilayer and charged groups polar interface. Here we examine relevance of this so-called "snorkeling" a cationic amino acid, which is conserved outer TMD small viral K+ channels. Experimentally, snorkeling activity not mandatory for KcvPBCV-1 K29 can be replaced most natural acids without any corruption function. Two similar channels, KcvATCV-1 KcvMT325, lack cytosolic N-terminus,...
The human immunodeficiency virus type 1 (HIV-1) protein Vpu is encoded exclusively by HIV-1 and related simian viruses (SIVs). transmembrane domain of the has dual functions: it counteracts restriction factor tetherin forms a cation channel. Since these two functions are causally unrelated remains unclear whether channel activity any relevance for viral release replication. Here we examine structure function correlates different homologs from SIV to understand if ion an evolutionary...