A5055135695- Fungal and yeast genetics research
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Liver Disease Diagnosis and Treatment
- Fermentation and Sensory Analysis
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Polyamine Metabolism and Applications
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Legume Nitrogen Fixing Symbiosis
- Cancer Cells and Metastasis
- Ion channel regulation and function
- Organ Transplantation Techniques and Outcomes
- Enzyme Structure and Function
- Plant Micronutrient Interactions and Effects
- Galectins and Cancer Biology
- Aluminum toxicity and tolerance in plants and animals
- Hepatitis C virus research
- Adipose Tissue and Metabolism
- Apelin-related biomedical research
- Metabolism, Diabetes, and Cancer
- Plant responses to water stress
- Mitochondrial Function and Pathology
- Hepatitis B Virus Studies
University of Córdoba
2016-2025
Cordoba University
2024
Institut de Recherche en Cancérologie de Montpellier
2020-2022
Institut Regional du Cancer de Montpellier
2020-2022
Leipzig University
2017-2022
Hôpital Gui de Chauliac
2010-2021
University of Freiburg
2020
Hôpital Saint Eloi
1998-2019
Centre Hospitalier Universitaire de Montpellier
2000-2019
Université de Montpellier
2006-2018
Circulating tumor cells (CTC) in blood are promising new biomarkers potentially useful for prognostic prediction and monitoring of therapies patients with solid tumors including colon cancer. Moreover, CTC research opens a avenue understanding the biology metastasis However, an in-depth investigation CTCs is hampered by very low number these cells, especially colorectal Thus, establishment cell cultures permanent lines from has become most challenging task over past year. Here, we describe,...
In a newly formulated growth medium lacking Na+ and NH4+, Saccharomyces cerevisiae grew maximally at 5 microM K+. Cells grown under these conditions transported K+ with an apparent Km of 24 microM, whereas cells in customary high-K+ had significantly higher (2 mM K+). The two types transport also differed carbonyl cyanide-m-chlorophenyl hydrazone sensitivity, response to ATP depletion, temperature dependence. results can be accounted for either by systems or one system operating different ways.
BACKGROUND The incidence and number of circulating tumor cells (CTCs) in the peripheral blood colorectal cancer patients are lower than other types, which may point to a particular biology affecting CTC detection. METHODS We detected CTCs mesenteric by use 2 independent technologies on basis different biological properties colon cells. Seventy-five diagnosed with localized (M0, n = 60) metastatic (M1, 15) were included. Peripheral samples collected before resection. performed enumeration an...
We recently identified a histological subtype of hepatocellular carcinoma (HCC), designated as "macrotrabecular-massive" (MTM-HCC) and associated with specific molecular features. In order to assess the clinical relevance this variant, we investigated its prognostic value in two large series patients HCC treated by either surgical resection or radiofrequency ablation (RFA). retrospectively included 237 samples 284 liver biopsies from RFA, respectively. Histological slides were reviewed...
The accumulation of compatible solutes, such as glycerol, in the yeast Saccharomyces cerevisiae , is a ubiquitous mechanism cellular osmoregulation. Here, we demonstrate that cells control glycerol part via regulated, Fps1p‐mediated export glycerol. Fps1p member MIP family channel proteins most closely related to bacterial facilitators. protein localized plasma membrane. physiological role appears be rather than uptake. Fps1Δ mutants are sensitive hypo‐osmotic shock, demonstrating osmolyte...
Null trk1 trk2 mutants of Saccharomyces cerevisiae exhibit a low-affinity uptake K+ and Rb+. We show that this Rb+ is mediated by several independent transporters, trk1Delta cells especially trk2Delta are highly hyperpolarized. Differences in the membrane potentials were assessed for sensitivity to hygromycin B flow cytometric analyses cellular DiOC6(3) fluorescence. On basis latter analyses, it proposed Trk1p Trk2p involved control potential, preventing excessive hyperpolarizations....
Phenotypic identification of focal nodular hyperplasia (FNH) and hepatocellular adenoma (HCA) subtypes using immunohistochemical markers has been developed from their molecular characteristics. Our objective was to evaluate the sensitivity these in definitive diagnosis lesions by core needle biopsies. A total 239 biopsies paired with surgical resection specimen (group A) or without an associated B) were reviewed. Using a step-by-step algorithm after standard staining, appropriate...
HER2-low breast cancer (i.e., HER 1+ or 2+, without gene amplification) is an emerging subtype for which very few data are available, especially within the triple-negative (TNBC) group. Our aim was to evaluate HER2 expression and its prognostic value in a large retrospective series of patients with non-metastatic TNBC (median age: 57.7 years; range: 28.5-98.6). Among 296 samples, 83.8% were 0, 13.5% 1+, 2.7% 2+ (HercepTestTM 2018 ASCO/CAP guidelines scoring). CK5/6 and/or EGFR-expressing...
Macrotrabecular-massive hepatocellular carcinoma (MTM-HCC) is a novel morphological subtype of HCC associated with early relapse after resection or percutaneous ablation, independently classical clinical and radiological prognostic factors. The aim the present study was to identify immunohistochemical markers MTM-HCC, ease its diagnosis implementation into practice.To potential biomarkers we first analyzed gene expression profiling data from Cancer Genome Atlas further selected two candidate...
The effects of KCl, NaCl, and LiCl on the growth Debaryomyces hansenii, usually considered a halotolerant yeast, Saccharomyces cerevisiae were compared. KCl NaCl had similar D. indicating that created only osmotic stress, while specific inhibitory effect, although relatively weaker than in S. cerevisiae. In media with low K+, Na+ was able to substitute for restoring rate final biomass culture. intracellular concentration reached values up 800 mM, suggesting metabolism is not affected by...
Summary Cells of the yeast Saccharomyces cerevisiae display a wide range glucose‐induced regulatory phenomena, including activation RAS‐adenylate cyclase pathway and phosphatidylinositol turnover, rapid post‐translational effects on activity different enzymes as well long‐term at transcriptional level. A gene called GGS1 (for General Glucose Sensor) that is apparently required for several ggs1 alleles ( fdp1, byp1 cif1 ) has been cloned characterized. homologue present in Methanobacterium...
A new YNB medium containing very low concentrations of alkali metal cations has been developed to carry out experiments study potassium homoeostasis. Physiological characterization Saccharomyces cerevisiae BY4741 strain and the corresponding mutant lacking main uptake systems (trk1 trk2) under nonlimiting limiting was performed, novel important differences between both strains were found. At KCl, two had a comparable cell size content. Nevertheless, mutants hyperpolarized, lower pH extruded...
ABSTRACT Wallemia ichthyophaga is a fungus from the ancient basidiomycetous genus (Wallemiales, Wallemiomycetes) that grows only at salinities between 10% (wt/vol) NaCl and saturated solution. This obligate halophily unique among fungi. The main goal of this study was to determine optimal salinity range for growth halophilic W. unravel its osmoadaptation strategy. Our results showed on solid media extremely slow resulted in small colonies. On other hand, liquid batch cultures, specific rates...
The primary endpoint was to analyze the predictive factors of lymph node involvement (LN+).Indications for additional right hemicolectomy (RHC) with (LN) resection after appendectomy appendix neuroendocrine tumor (A-NET) remain controversial, especially tumors between 1 and 2 cm in size.National study including all patients nonmetastatic A-NET diagnosed January, 2010 France.In all, 403 were included. A-NETs were: within tip (67%), body (24%) or base (9%) appendix; size < (62%), (30%), >2...