A5032458818- Porphyrin Metabolism and Disorders
- Folate and B Vitamins Research
- Heme Oxygenase-1 and Carbon Monoxide
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Neonatal Health and Biochemistry
- Photosynthetic Processes and Mechanisms
- Metalloenzymes and iron-sulfur proteins
- Cassava research and cyanide
- Metabolism and Genetic Disorders
- Iron Metabolism and Disorders
- Porphyrin and Phthalocyanine Chemistry
- Metal-Catalyzed Oxygenation Mechanisms
- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- Retinal Development and Disorders
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Bacteriophages and microbial interactions
- X-ray Diffraction in Crystallography
- Trace Elements in Health
- Legume Nitrogen Fixing Symbiosis
- Crystallization and Solubility Studies
- Algal biology and biofuel production
Quadram Institute
2020-2025
Norwich Research Park
2020-2025
University of Kent
2015-2024
University of East Anglia
2020-2024
St Vincent's Hospital
2018
University of Dallas
2016
Texas A&M University
1990-2016
Queen Mary University of London
1999-2013
Helmholtz Centre for Infection Research
2008
University College Cork
2008
Many algae are auxotrophs for vitamin B(12) (cobalamin), which they need as a cofactor -dependent methionine synthase (METH). Because only prokaryotes can synthesize the cobalamin, must be ultimate source of vitamin. In laboratory, direct interaction between and heterotrophic bacteria has been shown, with supplying cobalamin in exchange fixed carbon. Here we establish system to study this at molecular level. culture green alga Chlamydomonas nivalis, found contaminating bacterium, identified...
Eukaryotic microalgae and prokaryotic cyanobacteria are the major components of phytoplankton. Determining factors that govern growth these primary producers, how they interact, is therefore essential to understanding aquatic ecosystem productivity. Over half microalgal species representing marine freshwater habitats require for corrinoid cofactor B12, which synthesized de novo only by certain prokaryotes, including majority cyanobacteria. There several chemical variants not necessarily...
Targeting of proteins to bacterial microcompartments (BMCs) is mediated by an 18-amino-acid peptide sequence. Herein, we report the solution structure N-terminal targeting (P18) PduP, aldehyde dehydrogenase associated with 1,2-propanediol utilization metabolosome from Citrobacter freundii. The reveals have a well-defined helical conformation along its whole length. Saturation transfer difference and transferred NOE NMR has highlighted observed interaction surface on main interacting shell...
Many heterotrophic bacteria have the ability to make polyhedral structures containing metabolic enzymes that are bounded by a unilamellar protein shell (metabolosomes or enterosomes). These bacterial organelles contain associated with specific process (e.g. 1,2-propanediol ethanolamine utilization). We show 21 gene regulon specifying pdu organelle and propanediol utilization from Citrobacter freundii is fully functional when cloned in Escherichia coli, both producing metabolosomes allowing...
A Lactobacillus reuteri strain isolated from sourdough is known to produce the vitamin cobalamin. The organism requires this for glycerol cofermentation by a cobalamin-dependent enzyme, usually termed dehydratase, in synthesis of antimicrobial substance reuterin. We show that cobalamin-synthesizing capacity another L. (20016, type strain, human gut and recently sequenced as F275) genetically phenotypically linked, Enterobacteriaceae, production enzyme which associated with bacterial...
Modified tetrapyrroles such as chlorophyll, heme, siroheme, vitamin B 12 , coenzyme F 430 and heme d 1 underpin a wide range of essential biological functions in all domains life, it is therefore surprising that the syntheses many these life pigments remain poorly understood. It known construction central molecular framework modified mediated via common, core pathway. Herein further branch tetrapyrrole biosynthesis pathway described denitrifying sulfate-reducing bacteria well Archaea. This...
Bacterial microcompartments (BMCs) enhance the breakdown of metabolites such as 1,2-propanediol (1,2-PD) to propionic acid. The encapsulation proteins within BMC is mediated by presence targeting sequences. In an attempt redesign Pdu into a 1,2-PD synthesising factory using glycerol starting material we added N-terminal peptides dehydrogenase, dihydroxyacetone kinase, methylglyoxal synthase and oxidoreductase allow their inclusion empty BMC. producing strains containing fused enzymes exhibit...
Abstract Bacterial extracellular vesicles (BEVs) contribute to stress responses, quorum sensing, biofilm formation and interspecies interkingdom communication. However, the factors that regulate their release heterogeneity are not well understood. We set out investigate these in common gut commensal Bacteroides thetaiotaomicron by studying BEV throughout growth cycle. Utilising a range of methods, we demonstrate released at different stages have significantly composition, with early enriched...