A5020650768- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Monoclonal and Polyclonal Antibodies Research
- Toxin Mechanisms and Immunotoxins
- Influenza Virus Research Studies
- Glycosylation and Glycoproteins Research
- Photosynthetic Processes and Mechanisms
- Inhalation and Respiratory Drug Delivery
- Immune Response and Inflammation
- Escherichia coli research studies
- Clostridium difficile and Clostridium perfringens research
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Hemoglobin structure and function
- Antimicrobial Peptides and Activities
- Pneumocystis jirovecii pneumonia detection and treatment
- Antimicrobial Resistance in Staphylococcus
- Respiratory viral infections research
- Botulinum Toxin and Related Neurological Disorders
- Spectroscopy and Quantum Chemical Studies
- Infective Endocarditis Diagnosis and Management
- Protein purification and stability
- Mitochondrial Function and Pathology
- Advanced Drug Delivery Systems
- Electrochemical Analysis and Applications
National Institute for Biological Standards and Control
2014-2024
Government of the United Kingdom
2015
University of Hertfordshire
2012-2014
Medical Research Council
1994
King's College London
1991-1993
University of Kent
1993
University of St Andrews
1989-1991
University of Pennsylvania
1988-1989
Objectives: The purpose of this study was to further characterize the ultrafiltration (UF) method for determining free saccharide levels in glycoconjugate vaccines and compare it with other methods used determination meningococcal vaccines. Methods: We performed experiments on both glycoconjugates capsular polysaccharides, compared UF, deoxycholate (DOC) precipitation, solid-phase extraction (SPE) methods. Meningococcal polysaccharides from groups A (MenA), C (MenC), W (MenW) were...
The cytochrome bo quinol oxidase of Escherichia coli is one two respiratory O2 reductases which the bacterium synthesizes. enzyme complex contains copper and 2 mol b-type heme. Electron paramagnetic resonance (epr) spectroscopy membranes from a strain having amplified levels this reveals signals low- high-spin hemes, but copper, now established as component oxidase, not directly detectable by epr. signal complex, we attribute to o, when titrated potentiometrically, gives bell-shaped curve....
A physicochemical and immunological study of the stability three different meningococcal (Men) ACWY conjugate vaccines was performed to evaluate any patterns serogroup oligo- or polysaccharide-specific carrier protein-specific that would affect immunogenicity. Critical quality stability-indicating characteristics were measured, with supporting suitability both HPLC-SEC HPAEC-PAD methods detect changes following inappropriate vaccine storage. All final products, ACWY-CRM197, -DT -TT had...
In this work, we explore the effects of O-acetylation on physical and immunological characteristics WHO International Standards Vi polysaccharide (Vi) from both Citrobacter freundii Salmonella enterica serovar Typhi. We find that, although structurally identical according to NMR, two standards have differences with respect susceptibility de-O-acetylation viscosity in water. species equivalent mass O-acetylation-dependent binding a mouse monoclonal antibody anti-Vi polyclonal antisera,...
An analysis of structure-antibody recognition relationships in nine licenced polysaccharide-tetanus toxoid (TT) conjugate vaccines was performed. The panel conjugates used included vaccine components to protect against disease caused by Haemophilus influenzae type b, Neisseria meningitidis groups A, C, W and Y Streptococcus pneumoniae serotype 18C. Conformation structural size exclusion chromatography with multi-angle light scattering determine size, intrinsic fluorescence spectroscopy...
The cytochrome‐ bo quinol oxidase of Escherichia coli contains a high‐spin b ‐type heme (cytochrome o ), low‐spin ) and copper. EPR signal from cytochrome is axial high spin when titrated potentiometrically gives bell‐shaped curve. low‐potential side this curve ( E m7 approx. 160 mV) corresponds to the reduction/oxidation cytochrome. high‐potential 350 proposed be due copper center; in CuII form tight ‐copper coupling results net even system loss spectrum. Optical spectra α‐bands reduced...
Physico-chemical analysis of pneumococcal polysaccharide (PS)-protein conjugate vaccine components used for two commercially licensed vaccines was performed to compare the serotype- and carrier protein-specific stabilities these vaccines. Nineteen different monovalent conjugates from commercial utilizing CRM197, diphtheria toxoid (DT), Protein D (PD) or tetanus (TT) as proteins were incubated at temperatures up 56°C eight weeks subjected freeze-thawing (F/T). Structural stability evaluated...
Potentiometric analyses of the cytochrome o-type oxidase Escherichia coli, using membranes from a strain containing amplified levels bo complex, were conducted to resolve redox centres oxidase. The E. quinol oxidase, contains 2 mol b-type haem per complex and copper. Detailed analysis potentiometric titrations, based on absorbance Soret band, suggests that there are three contributions with midpoint potentials (Em,7) around +55 mV, +211 mV +408 all maxima at 426-430 nm in reduced state. In...
Towards achieving the goal of eliminating epidemic outbreaks meningococcal disease in African meningitis belt, a pentavalent glycoconjugate vaccine (NmCV-5) has been developed to protect against Neisseria meningitidis serogroups A, C, Y, W and X. MenA X polysaccharides are conjugated tetanus toxoid (TT) while MenC, Y recombinant cross reactive material 197 (rCRM197), non-toxic genetic variant diphtheria toxin. This study describes quality control testing performed by manufacturer, Serum...
ABSTRACT The phospholipases C of C. perfringens (alpha-toxin) and bifermentans (Cbp) show >50% amino acid homology but differ in their hemolytic toxic properties. We report here the purification characterisation alpha-toxin Cbp. phospholipase activity Cbp was similar when tested with phosphatidylcholine egg yolk or liposomes. However, more than 100-fold that To investigate whether differences carboxy-terminal domains these proteins were responsible for properties, a hybrid protein (N bi α...
A Haemophilus influenzae type‐ b capsular polysaccharide‐CRM 197 protein conjugate vaccine was compared with unconjugated CRM197 and diphtheria toxin, its parent molecule. Using CD fluorescence spectroscopy, it has been possible to observe differences in structure stability pH temperature due the G52→ mutation ‘glycosylation’ of conjugate. resembles ‘open’ conformation toxin [Blewitt, M. G., Chung, L. A. & London, E. (1985) Biochemistry 24 , 5458–5464] attachment poly(ribosyl‐ribitol...