A5051644130- Vector-borne infectious diseases
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Bacillus and Francisella bacterial research
- Insect and Pesticide Research
- Vibrio bacteria research studies
- Helicobacter pylori-related gastroenterology studies
- Galectins and Cancer Biology
- Escherichia coli research studies
- Yersinia bacterium, plague, ectoparasites research
- Gut microbiota and health
- Bacteriophages and microbial interactions
- Immune Response and Inflammation
- Clostridium difficile and Clostridium perfringens research
- Veterinary medicine and infectious diseases
- Bacterial Genetics and Biotechnology
- Hepatitis B Virus Studies
- Immunodeficiency and Autoimmune Disorders
- Eosinophilic Esophagitis
- Microbial Inactivation Methods
- Aquaculture disease management and microbiota
- Dermatological diseases and infestations
- Protein purification and stability
National Institute for Biological Standards and Control
2007-2021
Potters Bar Community Hospital
2020
University of Hertfordshire
2014-2016
Manchester Royal Infirmary
2009
Erasmus MC
2004
National Institute of Cholera and Enteric Diseases
2004
International Centre for Diarrhoeal Disease Research
2004
National Institute for Public Health and the Environment
1987-1999
The National Gallery
1998
Veterinary Medicines Directorate
1997-1998
A sensitive and specific PCR hybridization assay was developed for the simultaneous detection identification of Ehrlichia Borrelia burgdorferi sensu lato. In separate assays 16S rRNA gene species 23S-5S spacer region B. lato were amplified labeled by PCR. These products used in a reverse line blot which oligonucleotide probes are covalently linked to membrane parallel lines. Hybridization samples with on this enabled Ehrlichia, burgdorferi, Bartonella 40 different samples. The application...
ABSTRACT The genetic diversity of Borrelia burgdorferi sensu lato was assessed in a focus Lyme borreliosis southern Britain dominated by game birds. Ticks, rodents, and pheasants were analyzed for spirochete infections PCR targeting the 23S-5S rRNA genes, followed genotyping reverse line blot method. In questing Ixodes ricinus ticks, three genospecies B. detected, with highest prevalences found garinii valaisiana. stricto rare (<1%) all tick stages. afzelii not detected any samples. More...
We developed a rapid and reliable method for the identification Borrelia burgdorferi sensu lato species in ticks. used DNA sequence polymorphism of spacer region between 5S 23S rRNA genes, which has been shown to be able discriminate eight genomic groups B. (D. Postic, M. Assous, P. A. D. Grimont, G. Baranton, Int. J. Syst. Bacteriol. 44:743-752, 1994). Spacer was amplified by PCR then hybridized five membrane-bound oligonucleotides. The oligonucleotides were specific stricto, garinii,...
Effective standardisation of methodologies to analyse the microbiome is essential entire community. Despite field being established for over a decade, there are no accredited or certified reference materials available wider In this study, we describe development first reagents produced by National Institute Biological Standards and Control (NIBSC) analysis next-generation sequencing. These can act as global working standards will be evaluated candidate World Health Organization International...
The human gastric pathogen Helicobacter pylori expresses several putative outer-membrane proteins (OMPs), but the role of individual OMPs in colonization stomach by H. is still poorly understood. four such (AlpA, AlpB, OipA and HopZ) a guinea pig model infection has been investigated. Single alpA, alpB, hopZ oipA isogenic mutants were constructed pig-adapted, wild-type strain GP15. Guinea pigs inoculated intragastrically with strain, single or mixture mutant 1: 1 ratio. Three weeks after...
ABSTRACT Typhoid fever is a major public health problem in developing countries, conservatively estimated to occur 17 million cases and be responsible for 200,000 deaths annually. We investigated the acquisition of natural immunity Salmonella enterica serovar Typhi region where typhoid endemic by testing sera from an age-stratified sample 210 healthy participants Kathmandu, Nepal, bactericidal activity toward S . anti-Vi capsular polysaccharide antibodies. Bactericidal titers children were...
Understanding the variables that influence microbiome studies is critical for successful translational research. Inflammatory bowel disease (IBD) a complex group of diseases can present at multiple locations within Gastrointestinal tract. Here, using FAMISHED study cohort, we aimed to investigate relationship between IBD condition, location, and microbiome. Signatures microbiome, including measures diversity, taxonomy, functionality, all significantly differed across three different...
In this work, we explore the effects of O-acetylation on physical and immunological characteristics WHO International Standards Vi polysaccharide (Vi) from both Citrobacter freundii Salmonella enterica serovar Typhi. We find that, although structurally identical according to NMR, two standards have differences with respect susceptibility de-O-acetylation viscosity in water. species equivalent mass O-acetylation-dependent binding a mouse monoclonal antibody anti-Vi polyclonal antisera,...
Abstract Moderate to severe diarrhea caused by Shigella is a global health concern due its substantial contribution morbidity and mortality in children aged &lt;5 years low- middle-income countries. Although antibiotic treatment can be effective, emerging antimicrobial resistance, limited access, cost affirm the role of vaccines as most attractive countermeasure. Controlled human infection models (CHIMs) represent valuable tool for assessing vaccine efficacy potentially accelerating...
Unfed nymphal and adult Ixodes ricinus ticks were collected from five locations within the 10,000-ha Killarney National Park, Ireland. The distribution prevalence of genomospecies Borrelia burgdorferi sensu lato in investigated by PCR amplification intergenic spacer region between 5S 23S rRNA genes reverse line blotting with genomospecies-specific oligonucleotide probes. infected B. was significantly variable locations, ranging 11.5 to 28.9%. Four identified as stricto, afzelii, garinii,...
Vi capsular polysaccharide (Vi) conjugate vaccines, which can prevent typhoid in infants and young children, are being developed. Comparative immunogenicity studies facilitated by an International Standard (IS) for human anti-Vi IgG. 16/138, a pool of sera from volunteers received either vaccine or plain vaccine, was assessed as IS alongside U.S. reference reagent Vi-IgG
To analyze the presence of Borrelia burgdorferi sensu lato in synovial samples from knee joint patients with Lyme arthritis by polymerase chain reaction, and to differentiate species reverse line blot (RLB).Synovial fluid (SF) tissue (ST) were obtained (n = 4) various other forms 9). DNA extracted was amplified using, as a target, spacer region between 5S 23S ribosomal RNA genes B. lato. Subsequently, 4 species-specific probes used RLB for specific hybridization.DNA stricto detected SF ST 3...
Mice and guinea pigs were intranasally immunized with either recombinant lipoprotein 20 or Helicobacter pylori outer membrane vesicles (OMV). Cholera toxin was used as mucosal adjuvant. In mice, both vaccines elicited systemic local IgG responses, which correlated significantly lower levels of H. colonization. contrast, only OMV proved immunogenic in pigs, the development immune responses. These antibodies did not, however, correlate protection these animals, suggests that vaccine...
Abstract Staphylococcal enterotoxin B (SEB) is a bacterial superantigen that binds the receptors in APC/T cell synapse and causes increased proliferation of T cells cytokine storm syndrome vivo. Exposure to toxin can be lethal cause significant pathology humans. The lack effective therapies for SEB exposure remains an area concern, particularly scenarios acute mass casualties. We hypothesized blockade costimulatory signal by CTLA4-Ig synthetic protein (abatacept) could prevent SEB-dependent...