A5103074285- Asthma and respiratory diseases
- Mitochondrial Function and Pathology
- Nitric Oxide and Endothelin Effects
- Oral Health Pathology and Treatment
- Venomous Animal Envenomation and Studies
- SARS-CoV-2 and COVID-19 Research
- Adipose Tissue and Metabolism
- Chronic Obstructive Pulmonary Disease (COPD) Research
- CRISPR and Genetic Engineering
- Extracellular vesicles in disease
- Respiratory and Cough-Related Research
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Long-Term Effects of COVID-19
- ATP Synthase and ATPases Research
- Neuroscience of respiration and sleep
- Ion Channels and Receptors
- Shoulder Injury and Treatment
- COVID-19 Clinical Research Studies
- Cancer-related molecular mechanisms research
- Neonatal Respiratory Health Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cancer Genomics and Diagnostics
- IL-33, ST2, and ILC Pathways
- Healthcare and Venom Research
Jamia Millia Islamia
2016-2025
Ashoka University
2023
Weatherford College
2023
Institute of Genomics and Integrative Biology
2009-2023
University of Delhi
2023
All India Institute of Medical Sciences
2023
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2017-2022
Jinnah Postgraduate Medical Center
2021
Bahauddin Zakariya University
2020
Sher-i-Kashmir Institute of Medical Sciences
2017
Cigarette smoke (CS)-induced cellular senescence is involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). The molecular mechanism by which CS induces unknown. Here, we show that stress (exposure primary lung cells to extract 0.2-0.75% with a half-maximal inhibitory concentration ~0.5%) led impaired mitophagy and perinuclear accumulation damaged mitochondria associated both human fibroblasts small airway epithelial (SAECs). Impaired was attributed reduced Parkin...
Dynamic variations in mitochondrial shape have been related to function. However, tools automatically classify and enumerate shapes are lacking, as systematic studies exploring the relationship of such stress. Here we show that during increased generation reactive oxygen species (mtROS), mitochondria change their from tubular donut or blob forms, which can be computationally quantified. Imaging cells treated with rotenone antimycin, showed time dose-dependent conversion forms donut-shaped...
Airway epithelial injury is the hallmark of various respiratory diseases, but its mechanisms remain poorly understood. While 13-S-hydroxyoctadecadienoic acid (13-S-HODE) produced in high concentration during mitochondrial degradation reticulocytes little known about role asthma pathogenesis. Here, we show that extracellular 13-S-HODE induces dysfunction and airway apoptosis. This associated with features severe obstruction, lung remodeling, increase stress related proinflammatory cytokines...
A major bottleneck in scaling-up COVID-19 testing is the need for sophisticated instruments and well-trained healthcare professionals, which are already overwhelmed due to pandemic. Moreover, high-sensitive SARS-CoV-2 diagnostics contingent on an RNA extraction step, which, turn, restricted by constraints supply chain. Here, we present CASSPIT ( C as13 ssisted S aliva-based & martphone I ntegrated T esting), will allow direct use of saliva samples without extra step detection....
Circulating cell-free mitochondrial DNA (cf-mtDNA) has been found in the plasma of severely ill COVID-19 patients and is now known as a strong predictor mortality. However, underlying mechanism mtDNA release unexplored. Here, we show novel SARS-CoV-2-mediated pro-inflammatory/pro-apoptotic rational therapeutic stem cell-based approach to mitigate these effects. We systematically screened effects 29 SARS-CoV-2 proteins on damage cell death that NSP4 ORF9b caused extensive structural changes,...
MicroRNAs (miRs) regulate immunological pathways in health and disease, a number of miRs have been shown to be altered mouse models asthma. The secretion interleukin-10 (IL-10), an anti-inflammatory cytokine, has defective many inflammatory diseases including We recently demonstrated that miR-106a inhibits IL-10 post-transcriptional manner. In this study, we investigated the effect inhibition mmu-miR106a asthmatic condition find its possible role as therapeutic target. Our vitro experiments...
Oxidative and carbonyl stress is increased in lungs of smokers patients with chronic obstructive pulmonary disease (COPD), as well cigarette smoke (CS)-exposed rodent lungs. We previously showed that sirtuin1 (SIRT1), an antiaging protein, reduced CS-exposed mice COPD SIRT1 attenuates CS-induced lung inflammation injury. It not clear whether protects against oxidative stress. Therefore, we determined the effect on antioxidants response to CS exposure using loss- gain-of-function approaches,...
The mechanisms underlying asthmatic airway epithelial injury are not clear. 12/15-lipoxygenase (an ortholog of human 15-LOX-1), which is induced by IL-13, associated with mitochondrial degradation in reticulocytes at physiological conditions. In this study, we showed that 12/15-LOX expressed nonepithelial cells caused asthma pathogenesis. While overexpression or IL-13 administration to naïve mice injury, knockout/knockdown allergic reduced injury. constitutive expression 15-LOX-1 bronchial...
Oxygen-sensing prolyl-hydroxylase (PHD)-2 negatively regulates hypoxia-inducible factor (HIF)1-α and suppresses the hypoxic response. Hypoxia signaling is thought to be proinflammatory but also attenuates cellular injury apoptosis. Although increased response has been noted in asthma, its functional relevance unknown. The objectives of this study were dissect mechanisms role asthma pathophysiology. Experimental studies conducted mice using acute chronic allergic models asthma. allergically...
Abstract Daily oscillations of pulmonary function depend on the rhythmic activity circadian timing system. Environmental tobacco/cigarette smoke (CS) disrupts clock leading to enhanced inflammatory responses. Infection with influenza A virus (IAV) increases hospitalization rates and death in susceptible individuals, including patients Chronic Obstructive Pulmonary Disease (COPD). We hypothesized that molecular disruption is by IAV infection, altering cellular lung function, severity airway...
Abstract Discovery of exosomes as modulator cellular communication has added a new dimension to our understanding biological processes. Exosomes influence the systems by mediating trans-communication across tissues and cells, which important implication for health disease. In absence well-characterized modulators exosome biogenesis, an alternative option is target pathways generating exosomal components. Cholesterol represents one such essential component required biogenesis. We initiated...
To discover anticancer drugs with novel structures and expand our research scope, pyrazoline derivatives (3a-3l) were designed synthesized through cyclization of chalcones thiosemicarbazide/semicarbazide in CH3COOH as a solvent. All newly fully characterized using several spectroscopic experiments such 1H, 13C NMR, FT-IR spectroscopy, mass analysis. By HPLC, the purity all analogs was found above 95% both lead compounds (3a 3h) also validated by HRMS. Anticancer activity investigated MTT...
Mesenchymal stem cell (MSC) transplantation alleviates metabolic defects in diseased recipient cells by intercellular mitochondrial transport (IMT). However, the effect of host conditions on IMT and thereby therapeutic efficacy MSCs has largely remained unexplored. Here we found impaired mitophagy, reduced derived from high-fat diet (HFD)-induced obese mouse (MSC-Ob). MSC-Ob failed to sequester their damaged mitochondria into LC3-dependent autophagosomes due decrease cardiolipin content,...
Asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase (NOS), causes uncoupling NOS leading to generation reactive nitrogen species, such as peroxynitrite. The lung generates a significant amount ADMA, potentially contributing plasma ADMA levels that have been related endothelial dysfunction. infusion increased collagen deposition in lungs, suggesting it could influence the development chronic diseases fibrosis, obstructive pulmonary disease, and asthma. To...