A5102810534- interferon and immune responses
- Cytomegalovirus and herpesvirus research
- Hepatitis C virus research
- SARS-CoV-2 and COVID-19 Research
- Inflammasome and immune disorders
- Bone and Dental Protein Studies
- Immune Cell Function and Interaction
- Computational Drug Discovery Methods
- Hepatitis B Virus Studies
- Viral Infections and Vectors
- Protein Structure and Dynamics
- Biosensors and Analytical Detection
- Bone health and treatments
- Liver Disease Diagnosis and Treatment
- thermodynamics and calorimetric analyses
- CRISPR and Genetic Engineering
- Toxoplasma gondii Research Studies
- SARS-CoV-2 detection and testing
- Long-Term Effects of COVID-19
- Herpesvirus Infections and Treatments
- Cancer Genomics and Diagnostics
- Synthesis and Biological Evaluation
- Synthesis and biological activity
- Cholesterol and Lipid Metabolism
- Immunodeficiency and Autoimmune Disorders
Jamia Millia Islamia
2019-2025
Indira Gandhi Institute of Medical Sciences
2022
Rosalind Franklin University of Medicine and Science
2011-2018
Narayan Medical College and Hospital
2016
Interleukin-1β (IL-1β) is a potent pro-inflammatory cytokine involved in the pathogenesis of HCV, but sensors and underlying mechanisms that facilitate HCV-induced IL-1β proteolytic activation secretion remains unclear. In this study, we have identified signalling pathway leading to response HCV infection. Previous studies shown induction through inflammasome complex macrophages/monocytes. Here, report for first time assembly NALP3-inflammasome human hepatoma cells infected with (JFH-1). We...
The IL-1β and type I interferon-β (IFN-β) molecules are important inflammatory cytokines elicited by the eukaryotic host as innate immune responses against invading pathogens danger signals. Recently, a predominantly nuclear gamma-interferon-inducible protein 16 (IFI16) involved in transcriptional regulation has emerged an DNA sensor which induced IFN-β production through inflammasome STING activation, respectively. Herpesvirus (KSHV, EBV, HSV-1) episomal dsDNA genome recognition IFI16 leads...
The innate immune system pattern recognition receptors (PRR) are the first line of host defenses recognizing various pathogen- or danger-associated molecular patterns and eliciting by regulating production pro-inflammatory cytokines such as IL-1β, IL-18 interferon β (IFN-β). NOD-like (NLRs) AIM2-like (ALRs) cytoplasmic inflammasome sensors foreign molecules, including DNA. IFI16, a sequence-independent nuclear sensor ALR, recognizes episomal dsDNA genomes herpes viruses KSHV, EBV, HSV-1 in...
A major bottleneck in scaling-up COVID-19 testing is the need for sophisticated instruments and well-trained healthcare professionals, which are already overwhelmed due to pandemic. Moreover, high-sensitive SARS-CoV-2 diagnostics contingent on an RNA extraction step, which, turn, restricted by constraints supply chain. Here, we present CASSPIT ( C as13 ssisted S aliva-based & martphone I ntegrated T esting), will allow direct use of saliva samples without extra step detection....
Circulating cell-free mitochondrial DNA (cf-mtDNA) has been found in the plasma of severely ill COVID-19 patients and is now known as a strong predictor mortality. However, underlying mechanism mtDNA release unexplored. Here, we show novel SARS-CoV-2-mediated pro-inflammatory/pro-apoptotic rational therapeutic stem cell-based approach to mitigate these effects. We systematically screened effects 29 SARS-CoV-2 proteins on damage cell death that NSP4 ORF9b caused extensive structural changes,...
IFI16 (interferon gamma-inducible protein 16) recognizes nuclear episomal herpesvirus (Kaposi's sarcoma-associated [KSHV], Epstein-Barr virus [EBV], and herpes simplex 1 [HSV-1]) genomes induces the inflammasome interferon beta responses. It also acts as a lytic replication restriction factor inhibits viral DNA (human cytomegalovirus [HCMV] human papillomavirus [HPV]) transcription (HSV-1, HCMV, HPV) through epigenetic modifications of genomes. To date, role in biology latent viruses is not...
IFI16 (gamma-interferon-inducible protein 16), a predominantly nuclear involved in transcriptional regulation, also functions as an innate immune response DNA sensor and induces the IL-1β antiviral type-1 interferon-β (IFN-β) cytokines. We have shown that IFI16, association with BRCA1, sequence independent of episomal dsDNA genomes KSHV, EBV HSV-1. Recognition these herpesvirus resulted acetylation, BRCA1-IFI16-ASC-procaspase-1 inflammasome formation, cytoplasmic translocation, generation....
Hepatitis C virus (HCV) is a major human pathogen that requires better understanding of its interaction with host cells. There close association HCV life cycle lipid metabolism. Lipid droplets (LDs) have been found to be crucial organelles support replication and virion assembly. In addition their role in replication, LDs also protein-mediated antiviral properties are activated during infection. Studies shown replicates well cholesterol sphingolipid-rich membranes, but the ways which alters...
Abstract Hepatitis C virus (HCV) is a major global health issue, infecting over 170 million people worldwide and leading to severe liver diseases, including cirrhosis hepatocellular carcinoma. The ability of HCV persist cause chronic infection partly due its evasion the host’s innate immune responses, particularly those mediated by RIG-I-MAVS pathway, which critical for antiviral defense. Studies have shown crucial role genome sensing DNA viruses cyclic GMP-AMP synthase (cGAS) followed cGAMP...
Kaposi's sarcoma-associated herpesvirus (KSHV) binding to the endothelial cell surface heparan sulfate is followed by sequential interactions with α3β1, αVβ3 and αVβ5 integrins Ephrin A2 receptor tyrosine kinase (EphA2R). These activate host pre-existing FAK, Src, PI3-K RhoGTPase signaling cascades, c-Cbl mediated ubiquitination of receptors, recruitment CIB1, p130Cas Crk adaptor molecules, membrane bleb formation leading lipid raft dependent macropinocytosis KSHV into human microvascular...
Kaposi's sarcoma-associated herpesvirus (KSHV) enters human dermal microvascular endothelial cells (HMVEC-d), its naturalin vivotarget cells, by lipid raft-dependent macropinocytosis. The internalized viral envelope fuses with the macropinocytic membrane, and released capsid is transported to nuclear vicinity, resulting in entry of DNA. endosomal sorting complexes required for transport (ESCRT) proteins, which include ESCRT-0, -I, -II, -III, play a central role trafficking ubiquitinated...
Osteopontin (OPN) is a secreted phosphoprotein which has been linked to tumor progression and metastasis in variety of cancers including hepatocellular carcinoma (HCC). Previous studies have shown that OPN upregulated during liver injury inflammation. However, the role hepatitis C virus (HCV)-induced disease pathogenesis not known. In this study, we determined induction OPN, then investigated effect forms epithelial mesenchymal transition (EMT), migration invasion hepatocytes. We show mRNA...
Repurposing of antivirals is an attractive therapeutic option for the treatment COVID-19. Main protease (Mpro), also called 3 C-like (3CLpro) a key SARS-CoV-2 involved in viral replication, and promising drug target antivirals. A major challenge to test efficacy conformational plasticity Mpro its future mutation prone flexibility. Suitable choice drugs catalytic allosteric pockets appear be essential combination therapy. Current study, based on docking extensive set MD simulations, finds...
The development of sensitive and specific diagnostic tools for hepatitis B virus (HBV) C (HCV) remains crucial effective disease management control. In this study, we utilized CRISPR‐Cas12 CRISPR‐Cas13 systems the detection HBV (DNA virus) HCV (RNA virus), respectively. We designed tested multiple guide RNAs (gRNAs) targeting both viruses, confirming successful cleavage target sequences through gel electrophoresis a fluorescent reporter assay. Using optimized gRNAs, developed lateral flow...
ABSTRACT Hepatitis C virus (HCV) replication and assembly occur at the specialized site of endoplasmic reticulum (ER) membranes lipid droplets (LDs), respectively. Recently, several host proteins have been shown to be involved in HCV assembly. In present study, we demonstrated important relationship among osteopontin (OPN), ER, LDs. OPN is a secreted phosphoprotein, overexpression hepatocellular carcinoma (HCC) tissue can lead invasion metastasis. expression also enhanced HCV-associated HCC....
One of the most common presentations blunt and penetrating ocular trauma is traumatic cataract. It remains a significant cause visual impairment physical disability among adults children. associated with various injuries.To evaluate injuries final outcome patients cataract along their demographic features modes trauma.A prospective study done in Department Ophthalmology M.L.B. Medical college, Jhansi from February 2010 to July 2011. A total 48 diagnosed as case were subjected detailed...