A5088062656- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Photoreceptor and optogenetics research
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Advanced Fluorescence Microscopy Techniques
- Spectroscopy and Quantum Chemical Studies
- Advanced Electron Microscopy Techniques and Applications
- Genomics and Phylogenetic Studies
- Advanced X-ray Imaging Techniques
- Protein Structure and Dynamics
- Biofuel production and bioconversion
- Biochemical and Molecular Research
- RNA and protein synthesis mechanisms
- ATP Synthase and ATPases Research
- Porphyrin and Phthalocyanine Chemistry
- Studies on Chitinases and Chitosanases
- Computational Drug Discovery Methods
- Enzyme Catalysis and Immobilization
- Biofield Effects and Biophysics
- Chemical Synthesis and Analysis
- Hemoglobin structure and function
- Magnetic and Electromagnetic Effects
- Crystallization and Solubility Studies
- Digital Holography and Microscopy
University of Gothenburg
2014-2024
Stockholm University
2005-2007
Vanderbilt University
2007
University of Illinois Urbana-Champaign
2006
Michigan State University
2005
Abstract Carbohydrate-binding modules (CBMs) are non-catalytic proteins found appended to carbohydrate-active enzymes. Soil and marine bacteria secrete such enzymes scavenge nutrition, they often use CBMs improve reaction rates retention of released sugars. Here we present a structural functional analysis the recently established CBM family 92. All analysed bind preferentially β−1,6-glucans. This contrasts with diversity predicted substrates among attached CBM92 domains. We crystal...
Cytochrome c oxidase (C O) is the terminal enzyme of respiratory chain and couples energetically reduction oxygen to water proton pumping across membrane. The results from previous studies showed that can be uncoupled O 2 -reduction reaction by replacement one single residue, Asn-139 Asp (N139D), located ≈30 Å catalytic site, in D-proton pathway. uncoupling was correlated with an increase p K a internal donor, Glu-286, ≈9.4 >11. Here, we show acidic Asp-132 Asn N139D C (D132N/N139D...
The gut microbiota plays a central role in human health by enzymatically degrading dietary fiber and concomitantly excreting short chain fatty acids that are associated with manifold benefits. polysaccharide xylan is abundant but noncarbohydrate decorations hinder efficient cleavage glycoside hydrolases (GHs) need to be addressed carbohydrate esterases (CEs). Enzymes from esterase families 1 6 (CE1 6) perform key roles degradation removing feruloyl acetate decorations, yet little known about...
Tannins are secondary metabolites that enriched in the bark, roots, and knots trees known to hinder microbial attack. The biological degradation of water-soluble gallotannins, such as tannic acid, is initiated by tannase enzymes (EC 3.1.1.20), which esterases able liberate gallic acid from aromatic-sugar complexes. However, only few tannases have previously been studied detail. Here, for first time, we biochemically structurally characterize three a single organism, anaerobic bacterium...
In cytochrome c oxidase (CcO), exergonic electron transfer reactions from to oxygen drive proton pumping across the membrane. Elucidation of mechanism requires identification molecular components involved in and investigation coupling between internal CcO. While proton-input trajectory CcO is relatively well characterized, output pathway have not been identified detail. this study, we investigated pH dependence that are linked translocation a structural variant which Arg481, interacts with...
Cytochrome c oxidase catalyses the reduction of molecular oxygen to water while energy released in this process is used pump protons across a biological membrane. Although an extremely well-studied system, mechanism proton pumping by cytochrome still not understood. Here we report method produce large quantities highly diffracting microcrystals ba 3-type from Thermus thermophilus suitable for serial femtosecond crystallography. The room-temperature structure solved 2.3 Å resolution data...
Structure-based drug design is highly dependent on the availability of structures protein interest in complex with lead compounds. Ideally, this information can be used to guide chemical optimization a compound into pharmaceutical candidate. A limitation main structural method today - conventional X-ray crystallography that it only provides about its frozen state. Serial relatively new approach offers possibility study at room temperature (RT). Here, we explore use serial determine target,...
X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has allowed high-resolution structures to be determined from micro-meter sized crystals, whereas single particle coherent imaging requires development the resolution a few tens nanometers. Here we describe an intermediate approach: XFEL assemblies helical symmetry. We collected scattering...
Abstract Despite widespread public interest in the health impact of exposure to microwave radiation, studies influence radiation on biological samples are often inconclusive or contradictory. Here we examine frequencies 3.5 GHz, 20 GHz and 29 growth microtubules, which nanotubes that perform diverse functions eukaryotic cells. Since microtubules highly polar can extend several micrometres length, they predicted be sensitive non-ionizing radiation. Moreover, it has been speculated tubulin...
Serial crystallography is a rapidly growing method that can yield structural insights from microcrystals were previously considered to be too small useful in conventional X-ray crystallography. Here, conditions for of the photosynthetic reaction centre Blastochloris viridis within lipidic cubic phase (LCP) crystallization matrix employ seeding protocol utilizing detergent-grown crystals with different crystal packing are described. LCP diffracted 2.25 Å resolution when exposed XFEL...
Serial femtosecond crystallography was initially developed for room-temperature X-ray diffraction studies of macromolecules at free electron lasers. When combined with tools that initiate biological reactions within microcrystals, time-resolved serial allows the study structural changes occur during an enzyme catalytic reaction. synchrotron (SSX), which extends methods to radiation sources, is expanding scientific community using methods. This report presents a simple flow cell can be used...
Cytochrome c oxidase (C O) is part of the respiratory chain and contributes to electrochemical membrane gradient in mitochondria as well many bacteria, it uses energy released reduction oxygen pump protons across an energy-transducing biological membrane. Here, we use time-resolved serial femtosecond crystallography study structural response active site upon flash photolysis carbon monoxide (CO) from reduced heme a 3 ba -type C O. In contrast with aa enzyme, our data show how CO stabilized...
Cytochrome c oxidase is the terminal electron acceptor in respiratory chains of aerobic organisms and energetically couples reduction oxygen to water proton pumping across membrane. The mechanisms uptake, gating, have yet be completely elucidated at molecular level for these enzymes. For Rhodobacter sphaeroides CytcO (cytochrome aa3), it appears as though E286 side chain subunit I a branching point from which protons are shuttled either catalytic site O2 or pumped protons. Amide...
Over the last decade, serial crystallography, a method to collect complete diffraction datasets from large number of microcrystals delivered and exposed an X-ray beam in random orientations at room temperature, has been successfully implemented free-electron lasers synchrotron radiation facility beamlines. This development relies on growing variety sample presentation methods, including different fixed target supports, injection methods using gas-dynamic virtual-nozzle injectors...
Serial crystallography is having an increasing impact on structural biology. This emerging technique opens up new possibilities for studying protein structures at room temperature and investigating dynamics using time-resolved X-ray diffraction. A limitation of the method intrinsic need large quantities well ordered micrometre-sized crystals. Here, a presented to screen conditions that produce microcrystals membrane proteins in lipidic cubic phase well-based crystallization approach. key...